Probiotic <italic toggle="yes">Bifidobacterium lactis</italic> V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis

ABSTRACT Although a few studies have investigated the intestinal microbiota of women with polycystic ovary syndrome (PCOS), the functional and metabolic mechanisms of the microbes associated with PCOS, as well as potential microbial biomarkers, have not yet been identified. To address this gap, we d...

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Autores principales:	Jiachao Zhang, Zhihong Sun, Shuaiming Jiang, Xiaoye Bai, Chenchen Ma, Qiannan Peng, Kaining Chen, Haibo Chang, Tuanyu Fang, Heping Zhang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Bifidobacterium lactis V9 brain-gut axis intestinal microbiome polycystic ovary syndrome probiotics short-chain fatty acid Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dbefa9c21c364105a8c7672fd19b40f8
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spelling	oai:doaj.org-article:dbefa9c21c364105a8c7672fd19b40f82021-12-02T18:15:45ZProbiotic <italic toggle="yes">Bifidobacterium lactis</italic> V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis10.1128/mSystems.00017-192379-5077https://doaj.org/article/dbefa9c21c364105a8c7672fd19b40f82019-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00017-19https://doaj.org/toc/2379-5077ABSTRACT Although a few studies have investigated the intestinal microbiota of women with polycystic ovary syndrome (PCOS), the functional and metabolic mechanisms of the microbes associated with PCOS, as well as potential microbial biomarkers, have not yet been identified. To address this gap, we designed a two-phase experiment in which we performed shotgun metagenomic sequencing and monitored the metabolic parameters, gut-brain mediators, and sex hormones of PCOS patients. In the first stage, we identified an imbalance in the intestinal microbiota of the PCOS patients, observing that Faecalibacterium, Bifidobacterium, and Blautia were significantly more abundant in the control group, whereas Parabacteroides and Clostridium were enriched in the PCOS group. In the second stage, we monitored the impact of the probiotic Bifidobacterium lactis V9 on the intestinal microbiome, gut-brain mediators, and sex hormones of 14 PCOS patients. Notably, we observed that the levels of luteinizing hormone (LH) and LH/follicle-stimulating hormone (LH/FSH) decreased significantly in 9 volunteers, whereas the levels of sex hormones and intestinal short-chain fatty acids (SCFAs) increased markedly. In contrast, the changes in the indices mentioned above were indistinct in the remaining 5 volunteers. The results of an analysis of the number of viable Bifidobacterium lactis V9 cells in the two groups were highly consistent with the clinical and SCFA results. Therefore, effective host gut colonization of the probiotic Bifidobacterium lactis V9 was crucial for its ability to function as a probiotic. Finally, we propose a potential mechanism describing how probiotics regulate the levels of sex hormones by manipulating the intestinal microbiome in PCOS patients. IMPORTANCE Polycystic ovary syndrome (PCOS) is a common metabolic disorder among women of reproductive age worldwide. Through a two-phase clinical experiment, we first revealed an imbalance in the intestinal microbiome of PCOS patients. By binning and annotating shotgun metagenomic sequences into metagenomic species (MGS), 61 MGSs were identified as potential PCOS-related microbial biomarkers. In the second stage, we monitored the impact of the probiotic Bifidobacterium lactis V9 on the intestinal microbiota, metabolic parameters, gut-brain mediators, and sex hormones of PCOS patients. Notably, we observed that the PCOS-related clinical indices and the intestinal microbiotas of the participating patients exhibited an inconsistent response to the intake of the B. lactis V9 probiotic. Therefore, effective host gut colonization of the probiotic was crucial for its ability to function as a probiotic. Finally, we propose a potential mechanism by which B. lactis V9 regulates the levels of sex hormones by manipulating the intestinal microbiome in PCOS patients.Jiachao ZhangZhihong SunShuaiming JiangXiaoye BaiChenchen MaQiannan PengKaining ChenHaibo ChangTuanyu FangHeping ZhangAmerican Society for MicrobiologyarticleBifidobacterium lactis V9brain-gut axisintestinal microbiomepolycystic ovary syndromeprobioticsshort-chain fatty acidMicrobiologyQR1-502ENmSystems, Vol 4, Iss 2 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bifidobacterium lactis V9 brain-gut axis intestinal microbiome polycystic ovary syndrome probiotics short-chain fatty acid Microbiology QR1-502
spellingShingle	Bifidobacterium lactis V9 brain-gut axis intestinal microbiome polycystic ovary syndrome probiotics short-chain fatty acid Microbiology QR1-502 Jiachao Zhang Zhihong Sun Shuaiming Jiang Xiaoye Bai Chenchen Ma Qiannan Peng Kaining Chen Haibo Chang Tuanyu Fang Heping Zhang Probiotic <italic toggle="yes">Bifidobacterium lactis</italic> V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis
description	ABSTRACT Although a few studies have investigated the intestinal microbiota of women with polycystic ovary syndrome (PCOS), the functional and metabolic mechanisms of the microbes associated with PCOS, as well as potential microbial biomarkers, have not yet been identified. To address this gap, we designed a two-phase experiment in which we performed shotgun metagenomic sequencing and monitored the metabolic parameters, gut-brain mediators, and sex hormones of PCOS patients. In the first stage, we identified an imbalance in the intestinal microbiota of the PCOS patients, observing that Faecalibacterium, Bifidobacterium, and Blautia were significantly more abundant in the control group, whereas Parabacteroides and Clostridium were enriched in the PCOS group. In the second stage, we monitored the impact of the probiotic Bifidobacterium lactis V9 on the intestinal microbiome, gut-brain mediators, and sex hormones of 14 PCOS patients. Notably, we observed that the levels of luteinizing hormone (LH) and LH/follicle-stimulating hormone (LH/FSH) decreased significantly in 9 volunteers, whereas the levels of sex hormones and intestinal short-chain fatty acids (SCFAs) increased markedly. In contrast, the changes in the indices mentioned above were indistinct in the remaining 5 volunteers. The results of an analysis of the number of viable Bifidobacterium lactis V9 cells in the two groups were highly consistent with the clinical and SCFA results. Therefore, effective host gut colonization of the probiotic Bifidobacterium lactis V9 was crucial for its ability to function as a probiotic. Finally, we propose a potential mechanism describing how probiotics regulate the levels of sex hormones by manipulating the intestinal microbiome in PCOS patients. IMPORTANCE Polycystic ovary syndrome (PCOS) is a common metabolic disorder among women of reproductive age worldwide. Through a two-phase clinical experiment, we first revealed an imbalance in the intestinal microbiome of PCOS patients. By binning and annotating shotgun metagenomic sequences into metagenomic species (MGS), 61 MGSs were identified as potential PCOS-related microbial biomarkers. In the second stage, we monitored the impact of the probiotic Bifidobacterium lactis V9 on the intestinal microbiota, metabolic parameters, gut-brain mediators, and sex hormones of PCOS patients. Notably, we observed that the PCOS-related clinical indices and the intestinal microbiotas of the participating patients exhibited an inconsistent response to the intake of the B. lactis V9 probiotic. Therefore, effective host gut colonization of the probiotic was crucial for its ability to function as a probiotic. Finally, we propose a potential mechanism by which B. lactis V9 regulates the levels of sex hormones by manipulating the intestinal microbiome in PCOS patients.
format	article
author	Jiachao Zhang Zhihong Sun Shuaiming Jiang Xiaoye Bai Chenchen Ma Qiannan Peng Kaining Chen Haibo Chang Tuanyu Fang Heping Zhang
author_facet	Jiachao Zhang Zhihong Sun Shuaiming Jiang Xiaoye Bai Chenchen Ma Qiannan Peng Kaining Chen Haibo Chang Tuanyu Fang Heping Zhang
author_sort	Jiachao Zhang
title	Probiotic <italic toggle="yes">Bifidobacterium lactis</italic> V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis
title_short	Probiotic <italic toggle="yes">Bifidobacterium lactis</italic> V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis
title_full	Probiotic <italic toggle="yes">Bifidobacterium lactis</italic> V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis
title_fullStr	Probiotic <italic toggle="yes">Bifidobacterium lactis</italic> V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis
title_full_unstemmed	Probiotic <italic toggle="yes">Bifidobacterium lactis</italic> V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis
title_sort	probiotic <italic toggle="yes">bifidobacterium lactis</italic> v9 regulates the secretion of sex hormones in polycystic ovary syndrome patients through the gut-brain axis
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/dbefa9c21c364105a8c7672fd19b40f8
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Probiotic <italic toggle="yes">Bifidobacterium lactis</italic> V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis

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