Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury

Radiation injury will result in multiorgan dysfuntion leading to multiorgan failure. In addition to many factors such as radiation dose, dose rate, the severity of the injury will also depend on organ systems which are exposed. Here, we report the protective property of gamma tocotrienol (GT3) in to...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vidya P. Kumar, Sasha Stone, Shukla Biswas, Neel Sharma, Sanchita P. Ghosh
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2020
Materias:	gamma tocotrienol partial body irradiation small animal radiation research platform radiation injury to normal lungs prophylactic treatment Thoracic irradiation Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/dbfc01995d6a4714856cc667a1a96b88
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dbfc01995d6a4714856cc667a1a96b88
record_format	dspace
spelling	oai:doaj.org-article:dbfc01995d6a4714856cc667a1a96b882021-12-01T03:28:41ZGamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury1663-981210.3389/fphar.2020.587970https://doaj.org/article/dbfc01995d6a4714856cc667a1a96b882020-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2020.587970/fullhttps://doaj.org/toc/1663-9812Radiation injury will result in multiorgan dysfuntion leading to multiorgan failure. In addition to many factors such as radiation dose, dose rate, the severity of the injury will also depend on organ systems which are exposed. Here, we report the protective property of gamma tocotrienol (GT3) in total as well as partial body irradiation (PBI) model in C3H/HeN male mice. We have carried out PBI by targeting thoracic region (lung-PBI) using Small Animal Radiation Research Platform, an X-ray irradiator with capabilities of an image guided irradiation with a variable collimator with minimized exposure to non-targeted tissues and organs. Precise and accurate irradiation of lungs was carried out at either 14 or 16 Gy at an approximate dose rate of 2.6 Gy/min. Though a low throughput model, it is amenable to change the field size on the spot. No damage to other non-targeted organs was observed in histopathological evaluation. There was no significant change in peripheral blood counts of irradiated mice in comparison to naïve mice. Femoral bone marrow cells had no damage in irradiated mice. As expected, damage to the targeted tissue was observed in the histopathological evaluation and non-targeted tissue was found normal. Regeneration and increase of cellularity and megakaryocytes on GT3 treatment was compared to significant loss of cellularity in saline group. Peak alveolitis was observed on day 14 post-PBI and protection from alveolitis by GT3 was noted. In irradiated lung tissue, thirty proteins were found to be differentially expressed but modulated by GT3 to reverse the effects of irradiation. We propose that possible mode of action of GT3 could be Angiopoietin 2-Tie2 pathway leading to AKT/ERK pathways resulting in disruption in cell survival/angiogenesis.Vidya P. KumarSasha StoneShukla BiswasNeel SharmaSanchita P. GhoshFrontiers Media S.A.articlegamma tocotrienolpartial body irradiationsmall animal radiation research platformradiation injury to normal lungsprophylactic treatmentThoracic irradiationTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	gamma tocotrienol partial body irradiation small animal radiation research platform radiation injury to normal lungs prophylactic treatment Thoracic irradiation Therapeutics. Pharmacology RM1-950
spellingShingle	gamma tocotrienol partial body irradiation small animal radiation research platform radiation injury to normal lungs prophylactic treatment Thoracic irradiation Therapeutics. Pharmacology RM1-950 Vidya P. Kumar Sasha Stone Shukla Biswas Neel Sharma Sanchita P. Ghosh Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury
description	Radiation injury will result in multiorgan dysfuntion leading to multiorgan failure. In addition to many factors such as radiation dose, dose rate, the severity of the injury will also depend on organ systems which are exposed. Here, we report the protective property of gamma tocotrienol (GT3) in total as well as partial body irradiation (PBI) model in C3H/HeN male mice. We have carried out PBI by targeting thoracic region (lung-PBI) using Small Animal Radiation Research Platform, an X-ray irradiator with capabilities of an image guided irradiation with a variable collimator with minimized exposure to non-targeted tissues and organs. Precise and accurate irradiation of lungs was carried out at either 14 or 16 Gy at an approximate dose rate of 2.6 Gy/min. Though a low throughput model, it is amenable to change the field size on the spot. No damage to other non-targeted organs was observed in histopathological evaluation. There was no significant change in peripheral blood counts of irradiated mice in comparison to naïve mice. Femoral bone marrow cells had no damage in irradiated mice. As expected, damage to the targeted tissue was observed in the histopathological evaluation and non-targeted tissue was found normal. Regeneration and increase of cellularity and megakaryocytes on GT3 treatment was compared to significant loss of cellularity in saline group. Peak alveolitis was observed on day 14 post-PBI and protection from alveolitis by GT3 was noted. In irradiated lung tissue, thirty proteins were found to be differentially expressed but modulated by GT3 to reverse the effects of irradiation. We propose that possible mode of action of GT3 could be Angiopoietin 2-Tie2 pathway leading to AKT/ERK pathways resulting in disruption in cell survival/angiogenesis.
format	article
author	Vidya P. Kumar Sasha Stone Shukla Biswas Neel Sharma Sanchita P. Ghosh
author_facet	Vidya P. Kumar Sasha Stone Shukla Biswas Neel Sharma Sanchita P. Ghosh
author_sort	Vidya P. Kumar
title	Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury
title_short	Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury
title_full	Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury
title_fullStr	Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury
title_full_unstemmed	Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury
title_sort	gamma tocotrienol protects mice from targeted thoracic radiation injury
publisher	Frontiers Media S.A.
publishDate	2020
url	https://doaj.org/article/dbfc01995d6a4714856cc667a1a96b88
work_keys_str_mv	AT vidyapkumar gammatocotrienolprotectsmicefromtargetedthoracicradiationinjury AT sashastone gammatocotrienolprotectsmicefromtargetedthoracicradiationinjury AT shuklabiswas gammatocotrienolprotectsmicefromtargetedthoracicradiationinjury AT neelsharma gammatocotrienolprotectsmicefromtargetedthoracicradiationinjury AT sanchitapghosh gammatocotrienolprotectsmicefromtargetedthoracicradiationinjury
_version_	1718405930940366848

Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury

Ejemplares similares