Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genom...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/dbfc585081a74283ac54d99eb9b8f688 |
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| Sumario: | Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genome packaging is more efficient in insect cells compared to mammalian cells. |
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