Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genom...
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Nature Portfolio
2021
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oai:doaj.org-article:dbfc585081a74283ac54d99eb9b8f6882021-12-02T11:44:59ZVisualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host10.1038/s42003-021-01821-y2399-3642https://doaj.org/article/dbfc585081a74283ac54d99eb9b8f6882021-03-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01821-yhttps://doaj.org/toc/2399-3642Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genome packaging is more efficient in insect cells compared to mammalian cells.Erick Bermúdez-MéndezEugene A. KatrukhaCindy M. SpruitJeroen KortekaasPaul J. Wichgers SchreurNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021) |
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EN |
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Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 Erick Bermúdez-Méndez Eugene A. Katrukha Cindy M. Spruit Jeroen Kortekaas Paul J. Wichgers Schreur Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host |
description |
Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genome packaging is more efficient in insect cells compared to mammalian cells. |
format |
article |
author |
Erick Bermúdez-Méndez Eugene A. Katrukha Cindy M. Spruit Jeroen Kortekaas Paul J. Wichgers Schreur |
author_facet |
Erick Bermúdez-Méndez Eugene A. Katrukha Cindy M. Spruit Jeroen Kortekaas Paul J. Wichgers Schreur |
author_sort |
Erick Bermúdez-Méndez |
title |
Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host |
title_short |
Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host |
title_full |
Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host |
title_fullStr |
Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host |
title_full_unstemmed |
Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host |
title_sort |
visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/dbfc585081a74283ac54d99eb9b8f688 |
work_keys_str_mv |
AT erickbermudezmendez visualizingtheribonucleoproteincontentofsinglebunyavirusvirionsrevealsmoreefficientgenomepackaginginthearthropodhost AT eugeneakatrukha visualizingtheribonucleoproteincontentofsinglebunyavirusvirionsrevealsmoreefficientgenomepackaginginthearthropodhost AT cindymspruit visualizingtheribonucleoproteincontentofsinglebunyavirusvirionsrevealsmoreefficientgenomepackaginginthearthropodhost AT jeroenkortekaas visualizingtheribonucleoproteincontentofsinglebunyavirusvirionsrevealsmoreefficientgenomepackaginginthearthropodhost AT pauljwichgersschreur visualizingtheribonucleoproteincontentofsinglebunyavirusvirionsrevealsmoreefficientgenomepackaginginthearthropodhost |
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1718395313702567936 |