Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host

Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genom...

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Autores principales: Erick Bermúdez-Méndez, Eugene A. Katrukha, Cindy M. Spruit, Jeroen Kortekaas, Paul J. Wichgers Schreur
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/dbfc585081a74283ac54d99eb9b8f688
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spelling oai:doaj.org-article:dbfc585081a74283ac54d99eb9b8f6882021-12-02T11:44:59ZVisualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host10.1038/s42003-021-01821-y2399-3642https://doaj.org/article/dbfc585081a74283ac54d99eb9b8f6882021-03-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01821-yhttps://doaj.org/toc/2399-3642Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genome packaging is more efficient in insect cells compared to mammalian cells.Erick Bermúdez-MéndezEugene A. KatrukhaCindy M. SpruitJeroen KortekaasPaul J. Wichgers SchreurNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Erick Bermúdez-Méndez
Eugene A. Katrukha
Cindy M. Spruit
Jeroen Kortekaas
Paul J. Wichgers Schreur
Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
description Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genome packaging is more efficient in insect cells compared to mammalian cells.
format article
author Erick Bermúdez-Méndez
Eugene A. Katrukha
Cindy M. Spruit
Jeroen Kortekaas
Paul J. Wichgers Schreur
author_facet Erick Bermúdez-Méndez
Eugene A. Katrukha
Cindy M. Spruit
Jeroen Kortekaas
Paul J. Wichgers Schreur
author_sort Erick Bermúdez-Méndez
title Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
title_short Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
title_full Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
title_fullStr Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
title_full_unstemmed Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
title_sort visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/dbfc585081a74283ac54d99eb9b8f688
work_keys_str_mv AT erickbermudezmendez visualizingtheribonucleoproteincontentofsinglebunyavirusvirionsrevealsmoreefficientgenomepackaginginthearthropodhost
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