Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology

In this study, we proposed a simple and easy method for fabricating a three-dimensional (3D) structure that can recapitulate the morphology of a tissue surface and deliver biological molecules into complex-shaped target tissues. To fabricate the 3D hydrogel film structure, we utilized a direct tissu...

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Autores principales:	Young-Hyeon An, Su-Hwan Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	tissue structure molding catechol-modified alginate implantable scaffold local delivery cardiac tissue engineering Technology T Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dbfdcfef30f34278af24b508c06ad443
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spelling	oai:doaj.org-article:dbfdcfef30f34278af24b508c06ad4432021-11-25T16:46:28ZFacile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology10.3390/bioengineering81101642306-5354https://doaj.org/article/dbfdcfef30f34278af24b508c06ad4432021-10-01T00:00:00Zhttps://www.mdpi.com/2306-5354/8/11/164https://doaj.org/toc/2306-5354In this study, we proposed a simple and easy method for fabricating a three-dimensional (3D) structure that can recapitulate the morphology of a tissue surface and deliver biological molecules into complex-shaped target tissues. To fabricate the 3D hydrogel film structure, we utilized a direct tissue casting method that can recapitulate tissue structure in micro-/macroscale using polydimethylsiloxane (PDMS). A replica 3D negative mold was manufactured by a polyurethane acrylate (PUA)-based master mold. Then, we poured the catechol-conjugated alginate (ALG-C) solution into the mold and evaporated it to form a dried film, followed by crosslinking the film using calcium chloride. The ALG-C hydrogel film had a tensile modulus of 725.2 ± 123.4 kPa and maintained over 95% of initial weight after 1 week without significant degradation. The ALG-C film captured over 4.5 times as much macromolecule (FITC-dextran) compared to alginate film (ALG). The cardiomyoblast cells exhibited high cell viability over 95% on ALG-C film. Moreover, the ALG-C film had about 70% of surface-bound lentivirus (1% in ALG film), which finally exhibited much higher viral transfection efficiency of GFP protein to C2C12 cells on the film than ALG film. In conclusion, we demonstrated a 3D film structure of biofunctionalized hydrogel for substrate-mediated drug delivery, and this approach could be utilized to recapitulate the complex-shaped tissues.Young-Hyeon AnSu-Hwan KimMDPI AGarticletissue structure moldingcatechol-modified alginateimplantable scaffoldlocal deliverycardiac tissue engineeringTechnologyTBiology (General)QH301-705.5ENBioengineering, Vol 8, Iss 164, p 164 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tissue structure molding catechol-modified alginate implantable scaffold local delivery cardiac tissue engineering Technology T Biology (General) QH301-705.5
spellingShingle	tissue structure molding catechol-modified alginate implantable scaffold local delivery cardiac tissue engineering Technology T Biology (General) QH301-705.5 Young-Hyeon An Su-Hwan Kim Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology
description	In this study, we proposed a simple and easy method for fabricating a three-dimensional (3D) structure that can recapitulate the morphology of a tissue surface and deliver biological molecules into complex-shaped target tissues. To fabricate the 3D hydrogel film structure, we utilized a direct tissue casting method that can recapitulate tissue structure in micro-/macroscale using polydimethylsiloxane (PDMS). A replica 3D negative mold was manufactured by a polyurethane acrylate (PUA)-based master mold. Then, we poured the catechol-conjugated alginate (ALG-C) solution into the mold and evaporated it to form a dried film, followed by crosslinking the film using calcium chloride. The ALG-C hydrogel film had a tensile modulus of 725.2 ± 123.4 kPa and maintained over 95% of initial weight after 1 week without significant degradation. The ALG-C film captured over 4.5 times as much macromolecule (FITC-dextran) compared to alginate film (ALG). The cardiomyoblast cells exhibited high cell viability over 95% on ALG-C film. Moreover, the ALG-C film had about 70% of surface-bound lentivirus (1% in ALG film), which finally exhibited much higher viral transfection efficiency of GFP protein to C2C12 cells on the film than ALG film. In conclusion, we demonstrated a 3D film structure of biofunctionalized hydrogel for substrate-mediated drug delivery, and this approach could be utilized to recapitulate the complex-shaped tissues.
format	article
author	Young-Hyeon An Su-Hwan Kim
author_facet	Young-Hyeon An Su-Hwan Kim
author_sort	Young-Hyeon An
title	Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology
title_short	Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology
title_full	Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology
title_fullStr	Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology
title_full_unstemmed	Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology
title_sort	facile fabrication of three-dimensional hydrogel film with complex tissue morphology
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dbfdcfef30f34278af24b508c06ad443
work_keys_str_mv	AT younghyeonan facilefabricationofthreedimensionalhydrogelfilmwithcomplextissuemorphology AT suhwankim facilefabricationofthreedimensionalhydrogelfilmwithcomplextissuemorphology
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Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology

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