Expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.

<h4>Background</h4>MicroRNA expression is severely disrupted in carcinogenesis, however limited evidence is available validating results from cell-line models in human clinical cancer specimens. MicroRNA-21 (mir-21) and microRNA-143 (mir-143) have previously been identified as significan...

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Autores principales:	Georgios Deftereos, Simon R Corrie, Qinghua Feng, Janice Morihara, Joshua Stern, Stephen E Hawes, Nancy B Kiviat
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc0e4aba5fce45dda5061b2dcc0e226e
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spelling	oai:doaj.org-article:dc0e4aba5fce45dda5061b2dcc0e226e2021-11-18T07:32:21ZExpression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.1932-620310.1371/journal.pone.0028423https://doaj.org/article/dc0e4aba5fce45dda5061b2dcc0e226e2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22194833/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNA expression is severely disrupted in carcinogenesis, however limited evidence is available validating results from cell-line models in human clinical cancer specimens. MicroRNA-21 (mir-21) and microRNA-143 (mir-143) have previously been identified as significantly deregulated in a range of cancers including cervical cancer. Our goal was to investigate the expression patterns of several well-studied microRNA species in cervical samples and compare the results to cell line samples.<h4>Methodology/principal findings</h4>We measured the expression of mir-21 and mir-143 in 142 formalin-fixed, paraffin embedded (FFPE) cervical biopsy tissue blocks, collected from Dantec Oncology Clinic, Dakar, Senegal. MicroRNA expression analysis was performed using Taqman-based real-time PCR assays. Protein immunohistochemical staining was also performed to investigate target protein expression on 72 samples. We found that mir-21 expression increased with worsening clinical diagnosis but that mir-143 was not correlated with histology. These observations were in stark contrast to previous reports involving cervical cancer cell lines in which mir-143 was consistently down-regulated but mir-21 largely unaffected. We also identified, for the first time, that cytoplasmic expression of Programmed Cell Death Protein 4 PDCD4; a known target of mir-21) was significantly lower in women with invasive cervical carcinoma (ICC) in comparison to those with cervical intraepithelial neoplasia (2-3) or carcinoma in situ (CIN2-3/CIS), although there was no significant correlation between mir-21 and PDCD4 expression, despite previous studies identifying PDCD4 transcript as a known mir-21 target.<h4>Conclusions</h4>Whilst microRNA biomarkers have a number of promising features, more studies on expression levels in histologically defined clinical specimens are required to investigate clinical relevance of discovery-based studies. Mir-21 may be of some utility in predictive screening, given that we observed a significant correlation between mir-21 expression level and worsening histological diagnosis of cervical cancer.Georgios DeftereosSimon R CorrieQinghua FengJanice MoriharaJoshua SternStephen E HawesNancy B KiviatPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28423 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Georgios Deftereos Simon R Corrie Qinghua Feng Janice Morihara Joshua Stern Stephen E Hawes Nancy B Kiviat Expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.
description	<h4>Background</h4>MicroRNA expression is severely disrupted in carcinogenesis, however limited evidence is available validating results from cell-line models in human clinical cancer specimens. MicroRNA-21 (mir-21) and microRNA-143 (mir-143) have previously been identified as significantly deregulated in a range of cancers including cervical cancer. Our goal was to investigate the expression patterns of several well-studied microRNA species in cervical samples and compare the results to cell line samples.<h4>Methodology/principal findings</h4>We measured the expression of mir-21 and mir-143 in 142 formalin-fixed, paraffin embedded (FFPE) cervical biopsy tissue blocks, collected from Dantec Oncology Clinic, Dakar, Senegal. MicroRNA expression analysis was performed using Taqman-based real-time PCR assays. Protein immunohistochemical staining was also performed to investigate target protein expression on 72 samples. We found that mir-21 expression increased with worsening clinical diagnosis but that mir-143 was not correlated with histology. These observations were in stark contrast to previous reports involving cervical cancer cell lines in which mir-143 was consistently down-regulated but mir-21 largely unaffected. We also identified, for the first time, that cytoplasmic expression of Programmed Cell Death Protein 4 PDCD4; a known target of mir-21) was significantly lower in women with invasive cervical carcinoma (ICC) in comparison to those with cervical intraepithelial neoplasia (2-3) or carcinoma in situ (CIN2-3/CIS), although there was no significant correlation between mir-21 and PDCD4 expression, despite previous studies identifying PDCD4 transcript as a known mir-21 target.<h4>Conclusions</h4>Whilst microRNA biomarkers have a number of promising features, more studies on expression levels in histologically defined clinical specimens are required to investigate clinical relevance of discovery-based studies. Mir-21 may be of some utility in predictive screening, given that we observed a significant correlation between mir-21 expression level and worsening histological diagnosis of cervical cancer.
format	article
author	Georgios Deftereos Simon R Corrie Qinghua Feng Janice Morihara Joshua Stern Stephen E Hawes Nancy B Kiviat
author_facet	Georgios Deftereos Simon R Corrie Qinghua Feng Janice Morihara Joshua Stern Stephen E Hawes Nancy B Kiviat
author_sort	Georgios Deftereos
title	Expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.
title_short	Expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.
title_full	Expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.
title_fullStr	Expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.
title_full_unstemmed	Expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.
title_sort	expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/dc0e4aba5fce45dda5061b2dcc0e226e
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Expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer.

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