A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

ABSTRACT A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using a combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The database comprises a collection of nearly full-length 16S rRNA gene sequences f...

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Autores principales:	Susan Joseph, Joseph Aduse-Opoku, Ahmed Hashim, Eveliina Hanski, Ricarda Streich, Sarah C. L. Knowles, Amy B. Pedersen, William G. Wade, Michael A. Curtis
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	16S rRNA Streptococcus danieliae Apodemus sylvaticus database mouse models Mus musculus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dc0eb1dfd5f04ecf8b05acb8decb6b1e
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spelling	oai:doaj.org-article:dc0eb1dfd5f04ecf8b05acb8decb6b1e2021-12-02T19:36:37ZA 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community10.1128/mSystems.01222-202379-5077https://doaj.org/article/dc0eb1dfd5f04ecf8b05acb8decb6b1e2021-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.01222-20https://doaj.org/toc/2379-5077ABSTRACT A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using a combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The database comprises a collection of nearly full-length 16S rRNA gene sequences from cultured isolates and draft genomes from representative taxa collected from a range of sources, including specific-pathogen-free laboratory mice, wild Mus musculus domesticus mice, and formerly wild wood mouse Apodemus sylvaticus. At present, it comprises 103 mouse oral taxa (MOT) spanning four phyla—Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes—including 12 novel undescribed species-level taxa. The key observations from this study are (i) the low diversity and predominantly culturable nature of the laboratory mouse oral microbiome and (ii) the identification of three major murine-specific oral bacterial lineages, namely, Streptococcus danieliae (MOT10), Lactobacillus murinus (MOT93), and Gemella species 2 (MOT43), which is one of the novel, still-unnamed taxa. Of these, S. danieliae is of particular interest, since it is a major component of the oral microbiome from all strains of healthy and periodontally diseased laboratory mice, as well as being present in wild mice. It is expected that this well-characterized database should be a useful resource for in vitro experimentation and mouse model studies in the field of oral microbiology. IMPORTANCE Mouse model studies are frequently used in oral microbiome research, particularly to investigate diseases such as periodontitis and caries, as well as other related systemic diseases. We have reported here the details of the development of a curated reference database to characterize the oral microbial community in laboratory and some wild mice. The genomic information and findings reported here can help improve the outcomes and accuracy of host-microbe experimental studies that use murine models to understand health and disease. Work is also under way to make the reference data sets publicly available on a web server to enable easy access and downloading for researchers across the world.Susan JosephJoseph Aduse-OpokuAhmed HashimEveliina HanskiRicarda StreichSarah C. L. KnowlesAmy B. PedersenWilliam G. WadeMichael A. CurtisAmerican Society for Microbiologyarticle16S rRNAStreptococcus danieliaeApodemus sylvaticusdatabasemouse modelsMus musculusMicrobiologyQR1-502ENmSystems, Vol 6, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	16S rRNA Streptococcus danieliae Apodemus sylvaticus database mouse models Mus musculus Microbiology QR1-502
spellingShingle	16S rRNA Streptococcus danieliae Apodemus sylvaticus database mouse models Mus musculus Microbiology QR1-502 Susan Joseph Joseph Aduse-Opoku Ahmed Hashim Eveliina Hanski Ricarda Streich Sarah C. L. Knowles Amy B. Pedersen William G. Wade Michael A. Curtis A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community
description	ABSTRACT A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using a combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The database comprises a collection of nearly full-length 16S rRNA gene sequences from cultured isolates and draft genomes from representative taxa collected from a range of sources, including specific-pathogen-free laboratory mice, wild Mus musculus domesticus mice, and formerly wild wood mouse Apodemus sylvaticus. At present, it comprises 103 mouse oral taxa (MOT) spanning four phyla—Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes—including 12 novel undescribed species-level taxa. The key observations from this study are (i) the low diversity and predominantly culturable nature of the laboratory mouse oral microbiome and (ii) the identification of three major murine-specific oral bacterial lineages, namely, Streptococcus danieliae (MOT10), Lactobacillus murinus (MOT93), and Gemella species 2 (MOT43), which is one of the novel, still-unnamed taxa. Of these, S. danieliae is of particular interest, since it is a major component of the oral microbiome from all strains of healthy and periodontally diseased laboratory mice, as well as being present in wild mice. It is expected that this well-characterized database should be a useful resource for in vitro experimentation and mouse model studies in the field of oral microbiology. IMPORTANCE Mouse model studies are frequently used in oral microbiome research, particularly to investigate diseases such as periodontitis and caries, as well as other related systemic diseases. We have reported here the details of the development of a curated reference database to characterize the oral microbial community in laboratory and some wild mice. The genomic information and findings reported here can help improve the outcomes and accuracy of host-microbe experimental studies that use murine models to understand health and disease. Work is also under way to make the reference data sets publicly available on a web server to enable easy access and downloading for researchers across the world.
format	article
author	Susan Joseph Joseph Aduse-Opoku Ahmed Hashim Eveliina Hanski Ricarda Streich Sarah C. L. Knowles Amy B. Pedersen William G. Wade Michael A. Curtis
author_facet	Susan Joseph Joseph Aduse-Opoku Ahmed Hashim Eveliina Hanski Ricarda Streich Sarah C. L. Knowles Amy B. Pedersen William G. Wade Michael A. Curtis
author_sort	Susan Joseph
title	A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community
title_short	A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community
title_full	A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community
title_fullStr	A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community
title_full_unstemmed	A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community
title_sort	16s rrna gene and draft genome database for the murine oral bacterial community
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/dc0eb1dfd5f04ecf8b05acb8decb6b1e
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A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

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