The plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs

Abstract The AlcR fungal protein responds to ethanol and binds to the fungal pAlcA promoter in its presence. This system was transferred to plants over twenty years ago and was claimed to function in the same manner in plants. However, never has the control experiment with plants containing the AlcR...

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Autor principal:	Ricardo S. Randall
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc126f1a8dcc4cd6b4e7c0fabba36f58
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spelling	oai:doaj.org-article:dc126f1a8dcc4cd6b4e7c0fabba36f582021-12-02T13:57:26ZThe plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs10.1038/s41598-020-80903-z2045-2322https://doaj.org/article/dc126f1a8dcc4cd6b4e7c0fabba36f582021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80903-zhttps://doaj.org/toc/2045-2322Abstract The AlcR fungal protein responds to ethanol and binds to the fungal pAlcA promoter in its presence. This system was transferred to plants over twenty years ago and was claimed to function in the same manner in plants. However, never has the control experiment with plants containing the AlcR gene alone, with no downstream inducible construct, been made. In this paper, I conduct several experiments with this control, growing p35:AlcR plants in the presence or absence of ethanol. I found that when these plants were grown in the presence of ethanol, growth in several tissues and several stages of growth was retarded. This demonstrates that this system is not suitable for use in the plant sciences, and casts doubt on the conclusions of papers that have published phenotypes using this system.Ricardo S. RandallNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ricardo S. Randall The plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs
description	Abstract The AlcR fungal protein responds to ethanol and binds to the fungal pAlcA promoter in its presence. This system was transferred to plants over twenty years ago and was claimed to function in the same manner in plants. However, never has the control experiment with plants containing the AlcR gene alone, with no downstream inducible construct, been made. In this paper, I conduct several experiments with this control, growing p35:AlcR plants in the presence or absence of ethanol. I found that when these plants were grown in the presence of ethanol, growth in several tissues and several stages of growth was retarded. This demonstrates that this system is not suitable for use in the plant sciences, and casts doubt on the conclusions of papers that have published phenotypes using this system.
format	article
author	Ricardo S. Randall
author_facet	Ricardo S. Randall
author_sort	Ricardo S. Randall
title	The plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs
title_short	The plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs
title_full	The plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs
title_fullStr	The plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs
title_full_unstemmed	The plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs
title_sort	plant alcr-palca ethanol-inducible system displays gross growth artefacts independently of downstream palca-regulated inducible constructs
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dc126f1a8dcc4cd6b4e7c0fabba36f58
work_keys_str_mv	AT ricardosrandall theplantalcrpalcaethanolinduciblesystemdisplaysgrossgrowthartefactsindependentlyofdownstreampalcaregulatedinducibleconstructs AT ricardosrandall plantalcrpalcaethanolinduciblesystemdisplaysgrossgrowthartefactsindependentlyofdownstreampalcaregulatedinducibleconstructs
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The plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs

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