Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase

Acyl-homoserine lactone (AHL) is the most studied autoinducer in gram-negative bacteria controlling infections of various pathogens. Quenching of AHL signaling by inhibiting AHL synthesis or AHL-receptor binding via small molecular chemicals or enzymatically degrading AHL is commonly used to block b...

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Autores principales:	Jinxing Liao, Danyu Shen, Long Lin, Hongjun Chen, Yajie Jin, Shan-Ho Chou, Xiao-Quan Yu, Tao Li, Guoliang Qian
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Quorum sensing AHL Quorum quenching AHL synthase Autoinducer Lysobacter Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/dc12d11adef34e9a9b869a5b815693e4
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spelling	oai:doaj.org-article:dc12d11adef34e9a9b869a5b815693e42021-11-26T04:26:47ZBacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase2001-037010.1016/j.csbj.2021.11.017https://doaj.org/article/dc12d11adef34e9a9b869a5b815693e42021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2001037021004827https://doaj.org/toc/2001-0370Acyl-homoserine lactone (AHL) is the most studied autoinducer in gram-negative bacteria controlling infections of various pathogens. Quenching of AHL signaling by inhibiting AHL synthesis or AHL-receptor binding via small molecular chemicals or enzymatically degrading AHL is commonly used to block bacterial infections. Here, we describe a new quorum-quenching strategy that directly “acquires” bacterial genes/proteins through a defined platform. We artificially expressed a typical AHL synthase gene pcoI from the biocontrol Pseudomonas fluorescens 2P24 in the antifungal bacterium Lysobacter enzymogenes OH11 lacking AHL production. This step led to the discovery of multiple PcoI interacting protein candidates from L. enzymogenes. The individual expression of these candidate genes in 2P24 led to the identification of Le0959, which encodes leucyl aminopeptidase, an effective protein that inhibits AHL synthesis in 2P24. Therefore, we define Le0959 as LqqP (Lysobacter quorum-quenching protein). The expression of pcoI in E. coli could produce AHL, and the introduction of lqqP into E. coli expressing pcoI could prevent the production of AHL. LqqP directly binds to PcoI, and this protein–protein binding reduced the abundance of free PcoI (capable of AHL synthesis) in vivo, thereby blocking PcoI-dependent AHL production. Overall, this study highlights the discovery of LqqP in quenching AHL quorum sensing by binding to AHL synthase via developing a previously-uncharacterized screening technique for bacterial quorum quenching.Jinxing LiaoDanyu ShenLong LinHongjun ChenYajie JinShan-Ho ChouXiao-Quan YuTao LiGuoliang QianElsevierarticleQuorum sensingAHLQuorum quenchingAHL synthaseAutoinducerLysobacterBiotechnologyTP248.13-248.65ENComputational and Structural Biotechnology Journal, Vol 19, Iss , Pp 6179-6190 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Quorum sensing AHL Quorum quenching AHL synthase Autoinducer Lysobacter Biotechnology TP248.13-248.65
spellingShingle	Quorum sensing AHL Quorum quenching AHL synthase Autoinducer Lysobacter Biotechnology TP248.13-248.65 Jinxing Liao Danyu Shen Long Lin Hongjun Chen Yajie Jin Shan-Ho Chou Xiao-Quan Yu Tao Li Guoliang Qian Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase
description	Acyl-homoserine lactone (AHL) is the most studied autoinducer in gram-negative bacteria controlling infections of various pathogens. Quenching of AHL signaling by inhibiting AHL synthesis or AHL-receptor binding via small molecular chemicals or enzymatically degrading AHL is commonly used to block bacterial infections. Here, we describe a new quorum-quenching strategy that directly “acquires” bacterial genes/proteins through a defined platform. We artificially expressed a typical AHL synthase gene pcoI from the biocontrol Pseudomonas fluorescens 2P24 in the antifungal bacterium Lysobacter enzymogenes OH11 lacking AHL production. This step led to the discovery of multiple PcoI interacting protein candidates from L. enzymogenes. The individual expression of these candidate genes in 2P24 led to the identification of Le0959, which encodes leucyl aminopeptidase, an effective protein that inhibits AHL synthesis in 2P24. Therefore, we define Le0959 as LqqP (Lysobacter quorum-quenching protein). The expression of pcoI in E. coli could produce AHL, and the introduction of lqqP into E. coli expressing pcoI could prevent the production of AHL. LqqP directly binds to PcoI, and this protein–protein binding reduced the abundance of free PcoI (capable of AHL synthesis) in vivo, thereby blocking PcoI-dependent AHL production. Overall, this study highlights the discovery of LqqP in quenching AHL quorum sensing by binding to AHL synthase via developing a previously-uncharacterized screening technique for bacterial quorum quenching.
format	article
author	Jinxing Liao Danyu Shen Long Lin Hongjun Chen Yajie Jin Shan-Ho Chou Xiao-Quan Yu Tao Li Guoliang Qian
author_facet	Jinxing Liao Danyu Shen Long Lin Hongjun Chen Yajie Jin Shan-Ho Chou Xiao-Quan Yu Tao Li Guoliang Qian
author_sort	Jinxing Liao
title	Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase
title_short	Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase
title_full	Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase
title_fullStr	Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase
title_full_unstemmed	Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase
title_sort	bacterial quorum sensing quenching activity of lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/dc12d11adef34e9a9b869a5b815693e4
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Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase

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