Benchmarking tomographic acquisition schemes for high-resolution structural biology

Here the authors systematically benchmark cryo-electron tomography acquisition schemes to optimize the attainable resolution for subtomogram averaging, and find that dose-symmetric acquisition with even angular sampling provides a better outcome than most currently used acquisition schemes.

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Autores principales:	Beata Turoňová, Wim J. H. Hagen, Martin Obr, Shyamal Mosalaganti, J. Wouter Beugelink, Christian E. Zimmerli, Hans-Georg Kräusslich, Martin Beck
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/dc1b4bdb76a14aba9eff82a68c42333e
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spelling	oai:doaj.org-article:dc1b4bdb76a14aba9eff82a68c42333e2021-12-02T16:56:34ZBenchmarking tomographic acquisition schemes for high-resolution structural biology10.1038/s41467-020-14535-22041-1723https://doaj.org/article/dc1b4bdb76a14aba9eff82a68c42333e2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14535-2https://doaj.org/toc/2041-1723Here the authors systematically benchmark cryo-electron tomography acquisition schemes to optimize the attainable resolution for subtomogram averaging, and find that dose-symmetric acquisition with even angular sampling provides a better outcome than most currently used acquisition schemes.Beata TuroňováWim J. H. HagenMartin ObrShyamal MosalagantiJ. Wouter BeugelinkChristian E. ZimmerliHans-Georg KräusslichMartin BeckNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Beata Turoňová Wim J. H. Hagen Martin Obr Shyamal Mosalaganti J. Wouter Beugelink Christian E. Zimmerli Hans-Georg Kräusslich Martin Beck Benchmarking tomographic acquisition schemes for high-resolution structural biology
description	Here the authors systematically benchmark cryo-electron tomography acquisition schemes to optimize the attainable resolution for subtomogram averaging, and find that dose-symmetric acquisition with even angular sampling provides a better outcome than most currently used acquisition schemes.
format	article
author	Beata Turoňová Wim J. H. Hagen Martin Obr Shyamal Mosalaganti J. Wouter Beugelink Christian E. Zimmerli Hans-Georg Kräusslich Martin Beck
author_facet	Beata Turoňová Wim J. H. Hagen Martin Obr Shyamal Mosalaganti J. Wouter Beugelink Christian E. Zimmerli Hans-Georg Kräusslich Martin Beck
author_sort	Beata Turoňová
title	Benchmarking tomographic acquisition schemes for high-resolution structural biology
title_short	Benchmarking tomographic acquisition schemes for high-resolution structural biology
title_full	Benchmarking tomographic acquisition schemes for high-resolution structural biology
title_fullStr	Benchmarking tomographic acquisition schemes for high-resolution structural biology
title_full_unstemmed	Benchmarking tomographic acquisition schemes for high-resolution structural biology
title_sort	benchmarking tomographic acquisition schemes for high-resolution structural biology
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/dc1b4bdb76a14aba9eff82a68c42333e
work_keys_str_mv	AT beataturonova benchmarkingtomographicacquisitionschemesforhighresolutionstructuralbiology AT wimjhhagen benchmarkingtomographicacquisitionschemesforhighresolutionstructuralbiology AT martinobr benchmarkingtomographicacquisitionschemesforhighresolutionstructuralbiology AT shyamalmosalaganti benchmarkingtomographicacquisitionschemesforhighresolutionstructuralbiology AT jwouterbeugelink benchmarkingtomographicacquisitionschemesforhighresolutionstructuralbiology AT christianezimmerli benchmarkingtomographicacquisitionschemesforhighresolutionstructuralbiology AT hansgeorgkrausslich benchmarkingtomographicacquisitionschemesforhighresolutionstructuralbiology AT martinbeck benchmarkingtomographicacquisitionschemesforhighresolutionstructuralbiology
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Benchmarking tomographic acquisition schemes for high-resolution structural biology

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