<italic toggle="yes">Neisseria gonorrhoeae</italic> Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response

ABSTRACT Neisseria gonorrhoeae mounts a substantial transcriptional program in response to hydrogen peroxide (HP), a prominent reactive oxygen species (ROS) encountered during infection. We tested which strain FA1090 genes show differential transcript abundance in response to sublethal amounts of HP...

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Autores principales:	Sarah J. Quillin, Adam J. Hockenberry, Michael C. Jewett, H Steven Seifert
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Neisseria gonorrhoeae bleach gonorrhea hydrogen peroxide neutrophils superoxide Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dc1d87b862d94d9b88bc97b631c5fa1d
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spelling	oai:doaj.org-article:dc1d87b862d94d9b88bc97b631c5fa1d2021-12-02T18:39:46Z<italic toggle="yes">Neisseria gonorrhoeae</italic> Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response10.1128/mSystems.00156-182379-5077https://doaj.org/article/dc1d87b862d94d9b88bc97b631c5fa1d2018-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00156-18https://doaj.org/toc/2379-5077ABSTRACT Neisseria gonorrhoeae mounts a substantial transcriptional program in response to hydrogen peroxide (HP), a prominent reactive oxygen species (ROS) encountered during infection. We tested which strain FA1090 genes show differential transcript abundance in response to sublethal amounts of HP to differentiate HP-responsive signaling from widespread cellular death and dysregulation. RNA sequencing (RNA-Seq) revealed that 150 genes were significantly upregulated and 143 genes downregulated following HP exposure. We annotated HP-responsive operons and all transcriptional start sites (TSSs) and identified which TSSs responded to HP treatment. We compared the HP responses and other previously reported genes and found only partial overlapping of other regulatory networks, indicating that the response to HP involves multiple biological functions. Using a representative subset of responsive genes, we validated the RNA-Seq results and found that the HP transcriptome was similar to that of sublethal organic peroxide. None of the genes in the representative subset, however, responded to sublethal levels of HOCl or O2-. These results support the idea that N. gonorrhoeae may use variations in HP levels as a signal for different stages of infection. IMPORTANCE The strict human pathogen Neisseria gonorrhoeae is the only causative agent of the sexually transmitted disease gonorrhea. This bacterium encounters hydrogen peroxide produced from host cells during infection, but the organism survives in the presence of this antimicrobial agent. This work shows that the bacterium responds to hydrogen peroxide by regulating the expression of many genes involved in multiple processes.Sarah J. QuillinAdam J. HockenberryMichael C. JewettH Steven SeifertAmerican Society for MicrobiologyarticleNeisseria gonorrhoeaebleachgonorrheahydrogen peroxideneutrophilssuperoxideMicrobiologyQR1-502ENmSystems, Vol 3, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neisseria gonorrhoeae bleach gonorrhea hydrogen peroxide neutrophils superoxide Microbiology QR1-502
spellingShingle	Neisseria gonorrhoeae bleach gonorrhea hydrogen peroxide neutrophils superoxide Microbiology QR1-502 Sarah J. Quillin Adam J. Hockenberry Michael C. Jewett H Steven Seifert <italic toggle="yes">Neisseria gonorrhoeae</italic> Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response
description	ABSTRACT Neisseria gonorrhoeae mounts a substantial transcriptional program in response to hydrogen peroxide (HP), a prominent reactive oxygen species (ROS) encountered during infection. We tested which strain FA1090 genes show differential transcript abundance in response to sublethal amounts of HP to differentiate HP-responsive signaling from widespread cellular death and dysregulation. RNA sequencing (RNA-Seq) revealed that 150 genes were significantly upregulated and 143 genes downregulated following HP exposure. We annotated HP-responsive operons and all transcriptional start sites (TSSs) and identified which TSSs responded to HP treatment. We compared the HP responses and other previously reported genes and found only partial overlapping of other regulatory networks, indicating that the response to HP involves multiple biological functions. Using a representative subset of responsive genes, we validated the RNA-Seq results and found that the HP transcriptome was similar to that of sublethal organic peroxide. None of the genes in the representative subset, however, responded to sublethal levels of HOCl or O2-. These results support the idea that N. gonorrhoeae may use variations in HP levels as a signal for different stages of infection. IMPORTANCE The strict human pathogen Neisseria gonorrhoeae is the only causative agent of the sexually transmitted disease gonorrhea. This bacterium encounters hydrogen peroxide produced from host cells during infection, but the organism survives in the presence of this antimicrobial agent. This work shows that the bacterium responds to hydrogen peroxide by regulating the expression of many genes involved in multiple processes.
format	article
author	Sarah J. Quillin Adam J. Hockenberry Michael C. Jewett H Steven Seifert
author_facet	Sarah J. Quillin Adam J. Hockenberry Michael C. Jewett H Steven Seifert
author_sort	Sarah J. Quillin
title	<italic toggle="yes">Neisseria gonorrhoeae</italic> Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response
title_short	<italic toggle="yes">Neisseria gonorrhoeae</italic> Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response
title_full	<italic toggle="yes">Neisseria gonorrhoeae</italic> Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response
title_fullStr	<italic toggle="yes">Neisseria gonorrhoeae</italic> Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response
title_full_unstemmed	<italic toggle="yes">Neisseria gonorrhoeae</italic> Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response
title_sort	<italic toggle="yes">neisseria gonorrhoeae</italic> exposed to sublethal levels of hydrogen peroxide mounts a complex transcriptional response
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/dc1d87b862d94d9b88bc97b631c5fa1d
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<italic toggle="yes">Neisseria gonorrhoeae</italic> Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response

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