Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application

Chih-Chien Hu,1– 4 Chih-Hsiang Chang,1– 3 Yuhan Chang,1– 3 Jang-Hsing Hsieh,5,6 Steve Wen-Neng Ueng1– 3 1Bone and Joint Research Center, Chang Gung Memorial Hospital, Kweishan, Taoyuan 33305, Taiwan; 2Department of Orthopedic Surgery, Chang Gung Memorial Hospi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hu CC, Chang CH, Chang Y, Hsieh JH, Ueng SWN
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	silver nanoparticles ossification antibacterial ability biofilm Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dc23ededadbf41919d1e8699017aa281
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dc23ededadbf41919d1e8699017aa281
record_format	dspace
spelling	oai:doaj.org-article:dc23ededadbf41919d1e8699017aa2812021-12-02T11:11:07ZBeneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application1178-2013https://doaj.org/article/dc23ededadbf41919d1e8699017aa2812020-10-01T00:00:00Zhttps://www.dovepress.com/beneficial-effect-of-taon-ag-nanocomposite-titanium-on-antibacterial-c-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chih-Chien Hu,1– 4 Chih-Hsiang Chang,1– 3 Yuhan Chang,1– 3 Jang-Hsing Hsieh,5,6 Steve Wen-Neng Ueng1– 3 1Bone and Joint Research Center, Chang Gung Memorial Hospital, Kweishan, Taoyuan 33305, Taiwan; 2Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Kweishan, Taoyuan 33305, Taiwan; 3College of Medicine, Chang Gung University, Kweishan, Taoyuan 33302, Taiwan; 4Department of Orthopedic Surgery, Xiamen Chang Gung Hospital, Xiamen, Fujian, People’s Republic of China; 5Department of Materials Engineering, Ming Chi University of Technology, Taishan, Taipei 24301, Taiwan; 6Center for Thin Film Technologies and Applications, Ming Chi University of Technology, Taishan, Taipei 24301, TaiwanCorrespondence: Steve Wen-Neng UengDepartment of Orthopedic Surgery, Chang Gung Memorial Hospital, No. 5, Fu-Hsin St, Kweishan, Taoyuan 33305, TaiwanTel +88633281200-2420Fax +886 33278113Email wenneng@adm.cgmh.org.twPurpose: In this study, a novel oxygenated nanocomposite thin film, TaON-Ag, was investigated in vitro and in vivo to evaluate its biocompatibility and antibacterial ability.Material and Methods: The antibacterial ability of TaON-Ag nanocomposite-coated titanium (Ti) was evaluated using the Kirby-Bauer disk diffusion susceptibility test. The effects of TaON-Ag nanocomposite-coated metal on osteogenesis were further evaluated in an in vitro osteogenic culture model with rat marrow-derived mesenchymal stem cells (rMSCs). Furthermore, titanium rods coated with TaON-Ag were implanted into a rat femur fracture model either with or without osteomyelitis to investigate the effects of TaON-Ag in osteogenesis.Results: The TaON-Ag-coated Ti exhibited an effective antibacterial effect against Staphylococcus aureus, coagulase-negative Staphylococcus, and the Gram-negative strains Escherichia coli and Pseudomonas aeruginosa. Using an osteogenic culture with rMSCs and a rat femoral fracture model, the TaON-Ag-coated Ti did not interfere with the ossification of rMSCs in vitro or during fracture healing in vivo. Field-emission scanning electron microscopy (FE-SEM) revealed that coating with TaON-Ag could inhibit pathogen adhesion and biofilm formation in both Staphylococcus aureus and Escherichia coli.Conclusion: Using the proposed novel oxygenation process, TaON-Ag nanocomposite-coated Ti yielded robust biocompatibility and antibacterial ability against common microorganisms in orthopedic infections, thereby demonstrating potential for use in clinical applications.Keywords: silver nanoparticles, ossification, antibacterial ability, biofilmHu CCChang CHChang YHsieh JHUeng SWNDove Medical Pressarticlesilver nanoparticlesossificationantibacterial abilitybiofilmMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 7889-7900 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticles ossification antibacterial ability biofilm Medicine (General) R5-920
spellingShingle	silver nanoparticles ossification antibacterial ability biofilm Medicine (General) R5-920 Hu CC Chang CH Chang Y Hsieh JH Ueng SWN Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application
description	Chih-Chien Hu,1– 4 Chih-Hsiang Chang,1– 3 Yuhan Chang,1– 3 Jang-Hsing Hsieh,5,6 Steve Wen-Neng Ueng1– 3 1Bone and Joint Research Center, Chang Gung Memorial Hospital, Kweishan, Taoyuan 33305, Taiwan; 2Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Kweishan, Taoyuan 33305, Taiwan; 3College of Medicine, Chang Gung University, Kweishan, Taoyuan 33302, Taiwan; 4Department of Orthopedic Surgery, Xiamen Chang Gung Hospital, Xiamen, Fujian, People’s Republic of China; 5Department of Materials Engineering, Ming Chi University of Technology, Taishan, Taipei 24301, Taiwan; 6Center for Thin Film Technologies and Applications, Ming Chi University of Technology, Taishan, Taipei 24301, TaiwanCorrespondence: Steve Wen-Neng UengDepartment of Orthopedic Surgery, Chang Gung Memorial Hospital, No. 5, Fu-Hsin St, Kweishan, Taoyuan 33305, TaiwanTel +88633281200-2420Fax +886 33278113Email wenneng@adm.cgmh.org.twPurpose: In this study, a novel oxygenated nanocomposite thin film, TaON-Ag, was investigated in vitro and in vivo to evaluate its biocompatibility and antibacterial ability.Material and Methods: The antibacterial ability of TaON-Ag nanocomposite-coated titanium (Ti) was evaluated using the Kirby-Bauer disk diffusion susceptibility test. The effects of TaON-Ag nanocomposite-coated metal on osteogenesis were further evaluated in an in vitro osteogenic culture model with rat marrow-derived mesenchymal stem cells (rMSCs). Furthermore, titanium rods coated with TaON-Ag were implanted into a rat femur fracture model either with or without osteomyelitis to investigate the effects of TaON-Ag in osteogenesis.Results: The TaON-Ag-coated Ti exhibited an effective antibacterial effect against Staphylococcus aureus, coagulase-negative Staphylococcus, and the Gram-negative strains Escherichia coli and Pseudomonas aeruginosa. Using an osteogenic culture with rMSCs and a rat femoral fracture model, the TaON-Ag-coated Ti did not interfere with the ossification of rMSCs in vitro or during fracture healing in vivo. Field-emission scanning electron microscopy (FE-SEM) revealed that coating with TaON-Ag could inhibit pathogen adhesion and biofilm formation in both Staphylococcus aureus and Escherichia coli.Conclusion: Using the proposed novel oxygenation process, TaON-Ag nanocomposite-coated Ti yielded robust biocompatibility and antibacterial ability against common microorganisms in orthopedic infections, thereby demonstrating potential for use in clinical applications.Keywords: silver nanoparticles, ossification, antibacterial ability, biofilm
format	article
author	Hu CC Chang CH Chang Y Hsieh JH Ueng SWN
author_facet	Hu CC Chang CH Chang Y Hsieh JH Ueng SWN
author_sort	Hu CC
title	Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application
title_short	Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application
title_full	Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application
title_fullStr	Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application
title_full_unstemmed	Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application
title_sort	beneficial effect of taon-ag nanocomposite titanium on antibacterial capacity in orthopedic application
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/dc23ededadbf41919d1e8699017aa281
work_keys_str_mv	AT hucc beneficialeffectoftaonagnanocompositetitaniumonantibacterialcapacityinorthopedicapplication AT changch beneficialeffectoftaonagnanocompositetitaniumonantibacterialcapacityinorthopedicapplication AT changy beneficialeffectoftaonagnanocompositetitaniumonantibacterialcapacityinorthopedicapplication AT hsiehjh beneficialeffectoftaonagnanocompositetitaniumonantibacterialcapacityinorthopedicapplication AT uengswn beneficialeffectoftaonagnanocompositetitaniumonantibacterialcapacityinorthopedicapplication
_version_	1718396198428082176

Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application

Ejemplares similares