Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues

Abstract Cell adhesion mediated by selectins (expressed by activated endothelium, activated platelets, and leukocytes) binding to their resepective selectin ligands (expressed by cancer cells) may be involved in metastasis. Therefore, methods of characterizing selectin ligands expressed on human tis...

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Autores principales:	Eric W. Martin, Ramiro Malgor, Vicente A. Resto, Douglas J. Goetz, Monica M. Burdick
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc292edf5a4e49d8ac0208b3dfa32f21
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spelling	oai:doaj.org-article:dc292edf5a4e49d8ac0208b3dfa32f212021-12-02T15:07:55ZDynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues10.1038/s41598-019-44838-42045-2322https://doaj.org/article/dc292edf5a4e49d8ac0208b3dfa32f212019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44838-4https://doaj.org/toc/2045-2322Abstract Cell adhesion mediated by selectins (expressed by activated endothelium, activated platelets, and leukocytes) binding to their resepective selectin ligands (expressed by cancer cells) may be involved in metastasis. Therefore, methods of characterizing selectin ligands expressed on human tissue may serve as valuable assays. Presented herein is an innovative method for detecting functional selectin ligands expressed on human tissue that uses a dynamic approach, which allows for control over the force applied to the bonds between the probe and target molecules. This new method of tissue interrogation, known as dynamic biochemical tissue analysis (DBTA), involves the perfusion of molecular probe-coated microspheres over tissues. DBTA using selectin-coated probes is able to detect functional selectin ligands expressed on tissue from multiple cancer types at both primary and metastatic sites.Eric W. MartinRamiro MalgorVicente A. RestoDouglas J. GoetzMonica M. BurdickNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eric W. Martin Ramiro Malgor Vicente A. Resto Douglas J. Goetz Monica M. Burdick Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
description	Abstract Cell adhesion mediated by selectins (expressed by activated endothelium, activated platelets, and leukocytes) binding to their resepective selectin ligands (expressed by cancer cells) may be involved in metastasis. Therefore, methods of characterizing selectin ligands expressed on human tissue may serve as valuable assays. Presented herein is an innovative method for detecting functional selectin ligands expressed on human tissue that uses a dynamic approach, which allows for control over the force applied to the bonds between the probe and target molecules. This new method of tissue interrogation, known as dynamic biochemical tissue analysis (DBTA), involves the perfusion of molecular probe-coated microspheres over tissues. DBTA using selectin-coated probes is able to detect functional selectin ligands expressed on tissue from multiple cancer types at both primary and metastatic sites.
format	article
author	Eric W. Martin Ramiro Malgor Vicente A. Resto Douglas J. Goetz Monica M. Burdick
author_facet	Eric W. Martin Ramiro Malgor Vicente A. Resto Douglas J. Goetz Monica M. Burdick
author_sort	Eric W. Martin
title	Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
title_short	Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
title_full	Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
title_fullStr	Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
title_full_unstemmed	Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
title_sort	dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/dc292edf5a4e49d8ac0208b3dfa32f21
work_keys_str_mv	AT ericwmartin dynamicbiochemicaltissueanalysisdetectsfunctionalselectinligandsonhumancancertissues AT ramiromalgor dynamicbiochemicaltissueanalysisdetectsfunctionalselectinligandsonhumancancertissues AT vicentearesto dynamicbiochemicaltissueanalysisdetectsfunctionalselectinligandsonhumancancertissues AT douglasjgoetz dynamicbiochemicaltissueanalysisdetectsfunctionalselectinligandsonhumancancertissues AT monicamburdick dynamicbiochemicaltissueanalysisdetectsfunctionalselectinligandsonhumancancertissues
_version_	1718388351372886016

Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues

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