Evaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran

Background: Several studies have indicated that polymorphism in vitamin D pathway genes is associated with breast cancer (BC) risk. Vitamin D-binding protein (VDBP) is a vital element in the metabolism of the vitamin D. VDBP carries the serum 25(OH) D3 to cells to promote vitamin D biological functi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Roya Moloudinia, Gelavij Mahmoodi, Mohammad Abdi, Sabrieh Amini, Shirin Ferdowsi
Formato:	article
Lenguaje:	EN
Publicado:	Hamadan University of Medical Sciences 2020
Materias:	polymorphism vitamin d binding protein breast cancer Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/dc2d8d6559984821afd1c0db152b909f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dc2d8d6559984821afd1c0db152b909f
record_format	dspace
spelling	oai:doaj.org-article:dc2d8d6559984821afd1c0db152b909f2021-11-23T08:07:27ZEvaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran10.34172/ajmb.2020.132345-4113https://doaj.org/article/dc2d8d6559984821afd1c0db152b909f2020-12-01T00:00:00Zhttp://ajmb.umsha.ac.ir/PDF/ajmb-8-89.pdfhttps://doaj.org/toc/2345-4113Background: Several studies have indicated that polymorphism in vitamin D pathway genes is associated with breast cancer (BC) risk. Vitamin D-binding protein (VDBP) is a vital element in the metabolism of the vitamin D. VDBP carries the serum 25(OH) D3 to cells to promote vitamin D biological functions, such as cell proliferation and apoptosis. Missense SNP (rs.7041) is a common polymorphism in VDBP gene, which shows ethnic-specific allele frequencies. Objectives: This study presents the correlation of the rs7041 (Asp432Glu) gene polymorphism and plasma concentrations of VDBP in Kurdish patients with BC in Sanandaj, Iran. Methods: This cross-sectional study included 44 premenopausal BC patients and 44 healthy subjects. Plasma VDBP concentration was measured by enzyme-linked immunosorbent assay (ELISA). The VDBP (rs7041) was genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). Results: VDBP level was associated with a non-significant risk of BC (P=0.397). Frequencies of individuals with VDBP (rs7041) TT, TG, and GG genotypes were 13.6%, 52.2%, and 34.09% in case group and 11.3%, 79.5%, and 9.9% in control group, respectively. Genotype GG associated with increased susceptibility to developing BC (odds ratio [OR]=5.172, CI: 1.555-17.2, P=0.007). There was a significant reverse correlation between GT genotype and BC (OR=0.282, 95% CI: 0.110-0.722, P=0.008) Conclusion: The changes in the vitamin D pathway may increase susceptibility to develop BC in the Iranian Kurdish population.Roya MoloudiniaGelavij MahmoodiMohammad AbdiSabrieh AminiShirin FerdowsiHamadan University of Medical Sciencesarticlepolymorphismvitamin d binding proteinbreast cancerMedical technologyR855-855.5ENAvicenna Journal of Medical Biochemistry, Vol 8, Iss 2, Pp 89-93 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	polymorphism vitamin d binding protein breast cancer Medical technology R855-855.5
spellingShingle	polymorphism vitamin d binding protein breast cancer Medical technology R855-855.5 Roya Moloudinia Gelavij Mahmoodi Mohammad Abdi Sabrieh Amini Shirin Ferdowsi Evaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran
description	Background: Several studies have indicated that polymorphism in vitamin D pathway genes is associated with breast cancer (BC) risk. Vitamin D-binding protein (VDBP) is a vital element in the metabolism of the vitamin D. VDBP carries the serum 25(OH) D3 to cells to promote vitamin D biological functions, such as cell proliferation and apoptosis. Missense SNP (rs.7041) is a common polymorphism in VDBP gene, which shows ethnic-specific allele frequencies. Objectives: This study presents the correlation of the rs7041 (Asp432Glu) gene polymorphism and plasma concentrations of VDBP in Kurdish patients with BC in Sanandaj, Iran. Methods: This cross-sectional study included 44 premenopausal BC patients and 44 healthy subjects. Plasma VDBP concentration was measured by enzyme-linked immunosorbent assay (ELISA). The VDBP (rs7041) was genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). Results: VDBP level was associated with a non-significant risk of BC (P=0.397). Frequencies of individuals with VDBP (rs7041) TT, TG, and GG genotypes were 13.6%, 52.2%, and 34.09% in case group and 11.3%, 79.5%, and 9.9% in control group, respectively. Genotype GG associated with increased susceptibility to developing BC (odds ratio [OR]=5.172, CI: 1.555-17.2, P=0.007). There was a significant reverse correlation between GT genotype and BC (OR=0.282, 95% CI: 0.110-0.722, P=0.008) Conclusion: The changes in the vitamin D pathway may increase susceptibility to develop BC in the Iranian Kurdish population.
format	article
author	Roya Moloudinia Gelavij Mahmoodi Mohammad Abdi Sabrieh Amini Shirin Ferdowsi
author_facet	Roya Moloudinia Gelavij Mahmoodi Mohammad Abdi Sabrieh Amini Shirin Ferdowsi
author_sort	Roya Moloudinia
title	Evaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran
title_short	Evaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran
title_full	Evaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran
title_fullStr	Evaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran
title_full_unstemmed	Evaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran
title_sort	evaluation of vitamin d-binding protein gene polymorphism and its plasma concentration in kurdish patients with breast cancer in sanandaj, iran
publisher	Hamadan University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/dc2d8d6559984821afd1c0db152b909f
work_keys_str_mv	AT royamoloudinia evaluationofvitamindbindingproteingenepolymorphismanditsplasmaconcentrationinkurdishpatientswithbreastcancerinsanandajiran AT gelavijmahmoodi evaluationofvitamindbindingproteingenepolymorphismanditsplasmaconcentrationinkurdishpatientswithbreastcancerinsanandajiran AT mohammadabdi evaluationofvitamindbindingproteingenepolymorphismanditsplasmaconcentrationinkurdishpatientswithbreastcancerinsanandajiran AT sabriehamini evaluationofvitamindbindingproteingenepolymorphismanditsplasmaconcentrationinkurdishpatientswithbreastcancerinsanandajiran AT shirinferdowsi evaluationofvitamindbindingproteingenepolymorphismanditsplasmaconcentrationinkurdishpatientswithbreastcancerinsanandajiran
_version_	1718416854566830080

Evaluation of Vitamin D-Binding Protein Gene Polymorphism and its Plasma Concentration in Kurdish Patients With Breast Cancer in Sanandaj, Iran

Ejemplares similares