Overexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression

Abstract Ultraconserved regions (UCRs) are 481 genomic sequences with 100% identity across humans, rats, and mice. Increasing evidence suggests that non-coding RNAs transcribed from UCRs are involved in various diseases, especially cancers. The human transformer 2β gene (TRA2B) encodes a UCR (uc.138...

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Autores principales:	Yuki Kuwano, Kensei Nishida, Kazuhito Rokutan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc353caf2da04beabca761a3929fbb4b
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spelling	oai:doaj.org-article:dc353caf2da04beabca761a3929fbb4b2021-12-02T18:27:48ZOverexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression10.1038/s41598-021-88123-92045-2322https://doaj.org/article/dc353caf2da04beabca761a3929fbb4b2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88123-9https://doaj.org/toc/2045-2322Abstract Ultraconserved regions (UCRs) are 481 genomic sequences with 100% identity across humans, rats, and mice. Increasing evidence suggests that non-coding RNAs transcribed from UCRs are involved in various diseases, especially cancers. The human transformer 2β gene (TRA2B) encodes a UCR (uc.138) that spans exon 2 and its neighboring introns. TRA2B4 RNA is the only transcript that contains the whole exon 2 among five spliced TRA2B RNA variants (TRA2B1-5). TRA2B4 is upregulated in colon cancer cell lines, although it is not translated to Tra2β protein because of its nuclear retention. Nevertheless, the clinical significance and biological functions of uc.138 in colon cancer cells remain unclear. In this study, RNA in situ hybridization showed that TRA2B4 was predominantly overexpressed in the nucleus of colon adenocarcinoma and adenoma. Overexpression of TRA2B4 in colon cancer HCT116 cells promoted cell proliferation by changing the expression of G2/M-related cell cycle regulators. Moreover, TRA2B4 increased migration and cell viability in a uc.138 sequence-dependent manner. TRA2B4 significantly enhanced tumorigenesis in vivo. Taken together, uc.138 encoded in TRA2B4 plays an oncogenic role in tumor progression and may become a potential biomarker and therapeutic target in colon cancer.Yuki KuwanoKensei NishidaKazuhito RokutanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Kuwano Kensei Nishida Kazuhito Rokutan Overexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression
description	Abstract Ultraconserved regions (UCRs) are 481 genomic sequences with 100% identity across humans, rats, and mice. Increasing evidence suggests that non-coding RNAs transcribed from UCRs are involved in various diseases, especially cancers. The human transformer 2β gene (TRA2B) encodes a UCR (uc.138) that spans exon 2 and its neighboring introns. TRA2B4 RNA is the only transcript that contains the whole exon 2 among five spliced TRA2B RNA variants (TRA2B1-5). TRA2B4 is upregulated in colon cancer cell lines, although it is not translated to Tra2β protein because of its nuclear retention. Nevertheless, the clinical significance and biological functions of uc.138 in colon cancer cells remain unclear. In this study, RNA in situ hybridization showed that TRA2B4 was predominantly overexpressed in the nucleus of colon adenocarcinoma and adenoma. Overexpression of TRA2B4 in colon cancer HCT116 cells promoted cell proliferation by changing the expression of G2/M-related cell cycle regulators. Moreover, TRA2B4 increased migration and cell viability in a uc.138 sequence-dependent manner. TRA2B4 significantly enhanced tumorigenesis in vivo. Taken together, uc.138 encoded in TRA2B4 plays an oncogenic role in tumor progression and may become a potential biomarker and therapeutic target in colon cancer.
format	article
author	Yuki Kuwano Kensei Nishida Kazuhito Rokutan
author_facet	Yuki Kuwano Kensei Nishida Kazuhito Rokutan
author_sort	Yuki Kuwano
title	Overexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression
title_short	Overexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression
title_full	Overexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression
title_fullStr	Overexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression
title_full_unstemmed	Overexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression
title_sort	overexpression of the transcribed ultraconserved region uc.138 accelerates colon cancer progression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dc353caf2da04beabca761a3929fbb4b
work_keys_str_mv	AT yukikuwano overexpressionofthetranscribedultraconservedregionuc138acceleratescoloncancerprogression AT kenseinishida overexpressionofthetranscribedultraconservedregionuc138acceleratescoloncancerprogression AT kazuhitorokutan overexpressionofthetranscribedultraconservedregionuc138acceleratescoloncancerprogression
_version_	1718378066684674048

Overexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression

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