Effects of temperature on muscle growth and collagen deposition in zebrafish (Danio rerio)

Collagen is a major extracellular matrix component playing pivotal roles in maintaining the muscle growth and flesh texture of fish. Enhancement of collagen deposition in muscle by environmental factors serves as an effective way to improve quality of fish products. However, as a critical environmen...

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Autores principales:	Fan Lin, Jiali Lin, Xin Liu, Yuying Yuan, Guoquan Liu, Xiaokang Ye
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Temperature Zebrafish Muscle Collagen metabolism Aquaculture. Fisheries. Angling SH1-691
Acceso en línea:	https://doaj.org/article/dc3a95cf5e4546d8ae426dc914663e1b
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spelling	oai:doaj.org-article:dc3a95cf5e4546d8ae426dc914663e1b2021-11-20T05:07:23ZEffects of temperature on muscle growth and collagen deposition in zebrafish (Danio rerio)2352-513410.1016/j.aqrep.2021.100952https://doaj.org/article/dc3a95cf5e4546d8ae426dc914663e1b2022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352513421003690https://doaj.org/toc/2352-5134Collagen is a major extracellular matrix component playing pivotal roles in maintaining the muscle growth and flesh texture of fish. Enhancement of collagen deposition in muscle by environmental factors serves as an effective way to improve quality of fish products. However, as a critical environmental factor in aquaculture, the effects of temperature on muscle growth and collagen metabolism in fish are largely unknown. Here, we used zebrafish (Danio rerio) as a fish model to investigate the effects of water temperature (18 ℃, 28 ℃, 34 ℃) on the growth performance, muscle structure and collagen metabolism. After the 7-week trial, the growth performance and density of muscle fiber were significantly reduced in 18 ℃ and 34 ℃ groups, compared to the control group (28 ℃). While, the collagen deposition in muscle was significantly enhanced in low temperature group (18 ℃). RNA-sequencing and qRT-PCR indicated that the transcription of collagen genes (col1a1a, col1a1b, col2a1b, etc.), genes regulating collagen synthesis (tgf-β, ctgf) and degradation (mmp2, mmp9, timp2, etc.) were down-regulated as temperature increased. Furthermore, the protein processing in endoplasmic reticulum (ER) pathway was significantly affected by temperature. The transcription of molecular chaperones of procollagen (hsp47, p4hb, sec24a, etc) in the pathway was significantly up-regulated in high temperature group. Notably, no significant difference in the hydroxyproline content was observed among groups, suggesting a temperature compensation mechanism may exist during collagen post-translational modification (hydroxylation, etc.). It was concluded that temperature exerted profound effects on muscle growth and collagen metabolism in zebrafish. Appropriate low temperature was suggested to enhance collagen deposition in fish muscle during aquaculture practice. Our data would enrich basic knowledge of regulatory mechanism of collagen metabolism in fish and help to develop strategy for producing fish products with higher quality.Fan LinJiali LinXin LiuYuying YuanGuoquan LiuXiaokang YeElsevierarticleTemperatureZebrafishMuscleCollagen metabolismAquaculture. Fisheries. AnglingSH1-691ENAquaculture Reports, Vol 22, Iss , Pp 100952- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Temperature Zebrafish Muscle Collagen metabolism Aquaculture. Fisheries. Angling SH1-691
spellingShingle	Temperature Zebrafish Muscle Collagen metabolism Aquaculture. Fisheries. Angling SH1-691 Fan Lin Jiali Lin Xin Liu Yuying Yuan Guoquan Liu Xiaokang Ye Effects of temperature on muscle growth and collagen deposition in zebrafish (Danio rerio)
description	Collagen is a major extracellular matrix component playing pivotal roles in maintaining the muscle growth and flesh texture of fish. Enhancement of collagen deposition in muscle by environmental factors serves as an effective way to improve quality of fish products. However, as a critical environmental factor in aquaculture, the effects of temperature on muscle growth and collagen metabolism in fish are largely unknown. Here, we used zebrafish (Danio rerio) as a fish model to investigate the effects of water temperature (18 ℃, 28 ℃, 34 ℃) on the growth performance, muscle structure and collagen metabolism. After the 7-week trial, the growth performance and density of muscle fiber were significantly reduced in 18 ℃ and 34 ℃ groups, compared to the control group (28 ℃). While, the collagen deposition in muscle was significantly enhanced in low temperature group (18 ℃). RNA-sequencing and qRT-PCR indicated that the transcription of collagen genes (col1a1a, col1a1b, col2a1b, etc.), genes regulating collagen synthesis (tgf-β, ctgf) and degradation (mmp2, mmp9, timp2, etc.) were down-regulated as temperature increased. Furthermore, the protein processing in endoplasmic reticulum (ER) pathway was significantly affected by temperature. The transcription of molecular chaperones of procollagen (hsp47, p4hb, sec24a, etc) in the pathway was significantly up-regulated in high temperature group. Notably, no significant difference in the hydroxyproline content was observed among groups, suggesting a temperature compensation mechanism may exist during collagen post-translational modification (hydroxylation, etc.). It was concluded that temperature exerted profound effects on muscle growth and collagen metabolism in zebrafish. Appropriate low temperature was suggested to enhance collagen deposition in fish muscle during aquaculture practice. Our data would enrich basic knowledge of regulatory mechanism of collagen metabolism in fish and help to develop strategy for producing fish products with higher quality.
format	article
author	Fan Lin Jiali Lin Xin Liu Yuying Yuan Guoquan Liu Xiaokang Ye
author_facet	Fan Lin Jiali Lin Xin Liu Yuying Yuan Guoquan Liu Xiaokang Ye
author_sort	Fan Lin
title	Effects of temperature on muscle growth and collagen deposition in zebrafish (Danio rerio)
title_short	Effects of temperature on muscle growth and collagen deposition in zebrafish (Danio rerio)
title_full	Effects of temperature on muscle growth and collagen deposition in zebrafish (Danio rerio)
title_fullStr	Effects of temperature on muscle growth and collagen deposition in zebrafish (Danio rerio)
title_full_unstemmed	Effects of temperature on muscle growth and collagen deposition in zebrafish (Danio rerio)
title_sort	effects of temperature on muscle growth and collagen deposition in zebrafish (danio rerio)
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/dc3a95cf5e4546d8ae426dc914663e1b
work_keys_str_mv	AT fanlin effectsoftemperatureonmusclegrowthandcollagendepositioninzebrafishdaniorerio AT jialilin effectsoftemperatureonmusclegrowthandcollagendepositioninzebrafishdaniorerio AT xinliu effectsoftemperatureonmusclegrowthandcollagendepositioninzebrafishdaniorerio AT yuyingyuan effectsoftemperatureonmusclegrowthandcollagendepositioninzebrafishdaniorerio AT guoquanliu effectsoftemperatureonmusclegrowthandcollagendepositioninzebrafishdaniorerio AT xiaokangye effectsoftemperatureonmusclegrowthandcollagendepositioninzebrafishdaniorerio
_version_	1718419572027031552

Effects of temperature on muscle growth and collagen deposition in zebrafish (Danio rerio)

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