Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models

Jian Cheng1,*, Ying Zhou1,*, Baoan Chen1, Jun Wang1, Guohua Xia1, Nan Jin1, Jiahua Ding1, Chong Gao1, Gouming Chen2, Yushan Miao2, Weilan Li2, Ziling Liu3, Xuemei Wang11Department of Hematology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People&rsquo;s Republic of China...

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Autores principales:	Cheng J, Zhou Y, Chen B, Wang J, Xia G, Jin N, Ding J, Gao C, Chen G, Miao Y, Li W, Liu Z, Wang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dc3b5e6b54b749229290455b9e44c7d6
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spelling	oai:doaj.org-article:dc3b5e6b54b749229290455b9e44c7d62021-12-02T00:30:23ZPrevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models1176-91141178-2013https://doaj.org/article/dc3b5e6b54b749229290455b9e44c7d62011-10-01T00:00:00Zhttp://www.dovepress.com/prevention-of-acute-graft-versus-host-disease-by-magnetic-nanoparticle-a8402https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jian Cheng1,, Ying Zhou1,, Baoan Chen1, Jun Wang1, Guohua Xia1, Nan Jin1, Jiahua Ding1, Chong Gao1, Gouming Chen2, Yushan Miao2, Weilan Li2, Ziling Liu3, Xuemei Wang11Department of Hematology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People&rsquo;s Republic of China; 2Department of Pharmacy, Zhongda Hospital, Medical College, Southeast University, Nanjing, People&rsquo;s Republic of China; 3The First Hospital of Jilin University, Changchun, Jilin, People&rsquo;s Republic of China *These authors have contributed equally to this workObjective: To investigate the effect of magnetic nanoparticles (MNPs) of Fe3O4 combined with cyclosporin A (CsA) on acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in murine models.Methods: BALB/c mice preconditioned with total-body irradiation generated aGVHD and then were followed with allo-HSCT from allogeneic C57BL/6. Recipient mice were randomly divided into five groups and then given different supportive care and followed up. The physical signs and median survival time (MST) were recorded, peripheral blood cell counts were assessed, and histological changes of the main tissues were evaluated with hematoxylin-eosin staining. Furthermore, fluorescence polarization immunoassay was used to monitor the concentration of CsA.Results: The irradiated-only mice developed typical aGVHD, and the typical signs of aGVHD in the skin, liver, and intestine were observed by histopathological examination. Both CsA alone and in combination with Fe3O4 MNPs significantly prolonged the MST of recipient mice compared with both the control and the Fe3O4 MNPs groups. Notably, a combination of CsA with Fe3O4 MNPs can elevate the peripheral white blood cells and alleviate the symptoms of GVHD and the pathological damage after allo-HSCT. In addition, the concentration of CsA was higher in plasma, heart, liver, and spleen of recipient mice with supporting care of the combination of CsA with Fe3O4 MNPs than with CsA alone.Conclusion: Taken together, Fe3O4MNPs may be used as a carrier of immunosuppressive agents to alleviate GVHD after allo-HSCT in murine models.Keywords: allogenetic stem cell transplantation, mice, HSCTCheng JZhou YChen BWang JXia GJin NDing JGao CChen GMiao YLi WLiu ZWang XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2183-2189 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Cheng J Zhou Y Chen B Wang J Xia G Jin N Ding J Gao C Chen G Miao Y Li W Liu Z Wang X Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models
description	Jian Cheng1,, Ying Zhou1,, Baoan Chen1, Jun Wang1, Guohua Xia1, Nan Jin1, Jiahua Ding1, Chong Gao1, Gouming Chen2, Yushan Miao2, Weilan Li2, Ziling Liu3, Xuemei Wang11Department of Hematology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People&rsquo;s Republic of China; 2Department of Pharmacy, Zhongda Hospital, Medical College, Southeast University, Nanjing, People&rsquo;s Republic of China; 3The First Hospital of Jilin University, Changchun, Jilin, People&rsquo;s Republic of China *These authors have contributed equally to this workObjective: To investigate the effect of magnetic nanoparticles (MNPs) of Fe3O4 combined with cyclosporin A (CsA) on acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in murine models.Methods: BALB/c mice preconditioned with total-body irradiation generated aGVHD and then were followed with allo-HSCT from allogeneic C57BL/6. Recipient mice were randomly divided into five groups and then given different supportive care and followed up. The physical signs and median survival time (MST) were recorded, peripheral blood cell counts were assessed, and histological changes of the main tissues were evaluated with hematoxylin-eosin staining. Furthermore, fluorescence polarization immunoassay was used to monitor the concentration of CsA.Results: The irradiated-only mice developed typical aGVHD, and the typical signs of aGVHD in the skin, liver, and intestine were observed by histopathological examination. Both CsA alone and in combination with Fe3O4 MNPs significantly prolonged the MST of recipient mice compared with both the control and the Fe3O4 MNPs groups. Notably, a combination of CsA with Fe3O4 MNPs can elevate the peripheral white blood cells and alleviate the symptoms of GVHD and the pathological damage after allo-HSCT. In addition, the concentration of CsA was higher in plasma, heart, liver, and spleen of recipient mice with supporting care of the combination of CsA with Fe3O4 MNPs than with CsA alone.Conclusion: Taken together, Fe3O4MNPs may be used as a carrier of immunosuppressive agents to alleviate GVHD after allo-HSCT in murine models.Keywords: allogenetic stem cell transplantation, mice, HSCT
format	article
author	Cheng J Zhou Y Chen B Wang J Xia G Jin N Ding J Gao C Chen G Miao Y Li W Liu Z Wang X
author_facet	Cheng J Zhou Y Chen B Wang J Xia G Jin N Ding J Gao C Chen G Miao Y Li W Liu Z Wang X
author_sort	Cheng J
title	Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models
title_short	Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models
title_full	Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models
title_fullStr	Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models
title_full_unstemmed	Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models
title_sort	prevention of acute graft-versus-host disease by magnetic nanoparticles of fe3o4 combined with cyclosporin a in murine models
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/dc3b5e6b54b749229290455b9e44c7d6
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Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models

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