Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines
Zika virus (ZIKV) is a member of the Flaviviridae family, which includes other clinically notable viruses such as the 4 dengue virus serotypes (DENV-1–4). Distinguishing DENVs from ZIKV using the established serologic assays widely used for monitoring DENV transmission is difficult because of antib...
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Centers for Disease Control and Prevention
2021
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oai:doaj.org-article:dc3f161b10e44300a1e07f7361479c9c2021-11-22T12:57:17ZNovel Assay to Measure Seroprevalence of Zika Virus in the Philippines10.3201/eid2712.2111501080-60401080-6059https://doaj.org/article/dc3f161b10e44300a1e07f7361479c9c2021-12-01T00:00:00Zhttps://wwwnc.cdc.gov/eid/article/27/12/21-1150_articlehttps://doaj.org/toc/1080-6040https://doaj.org/toc/1080-6059 Zika virus (ZIKV) is a member of the Flaviviridae family, which includes other clinically notable viruses such as the 4 dengue virus serotypes (DENV-1–4). Distinguishing DENVs from ZIKV using the established serologic assays widely used for monitoring DENV transmission is difficult because of antibody cross-reactivity between these closely related flaviviruses. We describe a modified and improved recombinant envelope domain III–based serologic assay for detecting ZIKV type-specific antibodies in regions with endemic DENV transmission. When the assay was used to measure ZIKV seroprevalence in 2017 among children 9–14 years of age living in a region of the Philippines with endemic DENV transmission, we observed a ZIKV seroprevalence of 18%. Investigators should consider using the ZIKV envelope domain III–based assay, which is simple and readily adaptable for use in standard clinical and public health laboratories, to assess ZIKV seroprevalence in areas with endemic DENV transmission. Cameron AdamsRamesh JadiBruno Segovia-ChumbezJedas DaagMichelle YladeFreddy A. MedinaTyler M. SharpJorge L. Munoz-JordanIn-Kyu YoonJacqueline DeenAnna Lena LopezAravinda M. de SilvaLakshmanane PremkumarCenters for Disease Control and PreventionarticleZika virusarbovirusesdengue virusdiagnosticsELISA testingflavivirusesMedicineRInfectious and parasitic diseasesRC109-216ENEmerging Infectious Diseases, Vol 27, Iss 12, Pp 3073-3081 (2021) |
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DOAJ |
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Zika virus arboviruses dengue virus diagnostics ELISA testing flaviviruses Medicine R Infectious and parasitic diseases RC109-216 |
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Zika virus arboviruses dengue virus diagnostics ELISA testing flaviviruses Medicine R Infectious and parasitic diseases RC109-216 Cameron Adams Ramesh Jadi Bruno Segovia-Chumbez Jedas Daag Michelle Ylade Freddy A. Medina Tyler M. Sharp Jorge L. Munoz-Jordan In-Kyu Yoon Jacqueline Deen Anna Lena Lopez Aravinda M. de Silva Lakshmanane Premkumar Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines |
| description |
Zika virus (ZIKV) is a member of the Flaviviridae family, which includes other clinically notable viruses such as the 4 dengue virus serotypes (DENV-1–4). Distinguishing DENVs from ZIKV using the established serologic assays widely used for monitoring DENV transmission is difficult because of antibody cross-reactivity between these closely related flaviviruses. We describe a modified and improved recombinant envelope domain III–based serologic assay for detecting ZIKV type-specific antibodies in regions with endemic DENV transmission. When the assay was used to measure ZIKV seroprevalence in 2017 among children 9–14 years of age living in a region of the Philippines with endemic DENV transmission, we observed a ZIKV seroprevalence of 18%. Investigators should consider using the ZIKV envelope domain III–based assay, which is simple and readily adaptable for use in standard clinical and public health laboratories, to assess ZIKV seroprevalence in areas with endemic DENV transmission.
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article |
| author |
Cameron Adams Ramesh Jadi Bruno Segovia-Chumbez Jedas Daag Michelle Ylade Freddy A. Medina Tyler M. Sharp Jorge L. Munoz-Jordan In-Kyu Yoon Jacqueline Deen Anna Lena Lopez Aravinda M. de Silva Lakshmanane Premkumar |
| author_facet |
Cameron Adams Ramesh Jadi Bruno Segovia-Chumbez Jedas Daag Michelle Ylade Freddy A. Medina Tyler M. Sharp Jorge L. Munoz-Jordan In-Kyu Yoon Jacqueline Deen Anna Lena Lopez Aravinda M. de Silva Lakshmanane Premkumar |
| author_sort |
Cameron Adams |
| title |
Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines |
| title_short |
Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines |
| title_full |
Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines |
| title_fullStr |
Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines |
| title_full_unstemmed |
Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines |
| title_sort |
novel assay to measure seroprevalence of zika virus in the philippines |
| publisher |
Centers for Disease Control and Prevention |
| publishDate |
2021 |
| url |
https://doaj.org/article/dc3f161b10e44300a1e07f7361479c9c |
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