Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.

<h4>Background</h4>The present study aims to identify antigens in protein extracts of promastigote and amastigote-like Leishmania (Leishmania) chagasi syn. L. (L.) infantum recognized by antibodies present in the sera of dogs with asymptomatic and symptomatic visceral leishmaniasis (VL)....

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Autores principales:	Vinicio T S Coelho, Jamil S Oliveira, Diogo G Valadares, Miguel A Chávez-Fumagalli, Mariana C Duarte, Paula S Lage, Manuel Soto, Marcelo M Santoro, Carlos A P Tavares, Ana Paula Fernandes, Eduardo A F Coelho
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/dc416b86cf8d46cc80acdda37c4f26e6
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spelling	oai:doaj.org-article:dc416b86cf8d46cc80acdda37c4f26e62021-11-18T09:14:32ZIdentification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.1935-27271935-273510.1371/journal.pntd.0001430https://doaj.org/article/dc416b86cf8d46cc80acdda37c4f26e62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22272364/pdf/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>The present study aims to identify antigens in protein extracts of promastigote and amastigote-like Leishmania (Leishmania) chagasi syn. L. (L.) infantum recognized by antibodies present in the sera of dogs with asymptomatic and symptomatic visceral leishmaniasis (VL).<h4>Methodology/principal findings</h4>Proteins recognized by sera samples were separated by two-dimensional electrophoresis (2DE) and identified by mass spectrometry. A total of 550 spots were observed in the 2DE gels, and approximately 104 proteins were identified. Several stage-specific proteins could be identified by either or both classes of sera, including, as expected, previously known proteins identified as diagnosis, virulence factors, drug targets, or vaccine candidates. Three, seven, and five hypothetical proteins could be identified in promastigote antigenic extracts; while two, eleven, and three hypothetical proteins could be identified in amastigote-like antigenic extracts by asymptomatic and symptomatic sera, as well as a combination of both, respectively.<h4>Conclusions/significance</h4>The present study represents a significant contribution not only in identifying stage-specific L. infantum molecules, but also in revealing the expression of a large number of hypothetical proteins. Moreover, when combined, the identified proteins constitute a significant source of information for the improvement of diagnostic tools and/or vaccine development to VL.Vinicio T S CoelhoJamil S OliveiraDiogo G ValadaresMiguel A Chávez-FumagalliMariana C DuartePaula S LageManuel SotoMarcelo M SantoroCarlos A P TavaresAna Paula FernandesEduardo A F CoelhoPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 6, Iss 1, p e1430 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Vinicio T S Coelho Jamil S Oliveira Diogo G Valadares Miguel A Chávez-Fumagalli Mariana C Duarte Paula S Lage Manuel Soto Marcelo M Santoro Carlos A P Tavares Ana Paula Fernandes Eduardo A F Coelho Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.
description	<h4>Background</h4>The present study aims to identify antigens in protein extracts of promastigote and amastigote-like Leishmania (Leishmania) chagasi syn. L. (L.) infantum recognized by antibodies present in the sera of dogs with asymptomatic and symptomatic visceral leishmaniasis (VL).<h4>Methodology/principal findings</h4>Proteins recognized by sera samples were separated by two-dimensional electrophoresis (2DE) and identified by mass spectrometry. A total of 550 spots were observed in the 2DE gels, and approximately 104 proteins were identified. Several stage-specific proteins could be identified by either or both classes of sera, including, as expected, previously known proteins identified as diagnosis, virulence factors, drug targets, or vaccine candidates. Three, seven, and five hypothetical proteins could be identified in promastigote antigenic extracts; while two, eleven, and three hypothetical proteins could be identified in amastigote-like antigenic extracts by asymptomatic and symptomatic sera, as well as a combination of both, respectively.<h4>Conclusions/significance</h4>The present study represents a significant contribution not only in identifying stage-specific L. infantum molecules, but also in revealing the expression of a large number of hypothetical proteins. Moreover, when combined, the identified proteins constitute a significant source of information for the improvement of diagnostic tools and/or vaccine development to VL.
format	article
author	Vinicio T S Coelho Jamil S Oliveira Diogo G Valadares Miguel A Chávez-Fumagalli Mariana C Duarte Paula S Lage Manuel Soto Marcelo M Santoro Carlos A P Tavares Ana Paula Fernandes Eduardo A F Coelho
author_facet	Vinicio T S Coelho Jamil S Oliveira Diogo G Valadares Miguel A Chávez-Fumagalli Mariana C Duarte Paula S Lage Manuel Soto Marcelo M Santoro Carlos A P Tavares Ana Paula Fernandes Eduardo A F Coelho
author_sort	Vinicio T S Coelho
title	Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.
title_short	Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.
title_full	Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.
title_fullStr	Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.
title_full_unstemmed	Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.
title_sort	identification of proteins in promastigote and amastigote-like leishmania using an immunoproteomic approach.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/dc416b86cf8d46cc80acdda37c4f26e6
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Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.

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