BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors

Base editors allow for precision engineering of the genome. Here, the authors present BEAR, a plasmid-based fluorescence assay for the measurement of CBE and ABE activity, to reveal the mechanism underlying their differences and to increase the yield of edited cells with reduced indel background.

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Detalles Bibliográficos
Autores principales:	András Tálas, Dorottya A. Simon, Péter I. Kulcsár, Éva Varga, Sarah L. Krausz, Ervin Welker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/dc4c7d49e7c84512854b815f71269ae5
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spelling	oai:doaj.org-article:dc4c7d49e7c84512854b815f71269ae52021-11-08T11:11:59ZBEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors10.1038/s41467-021-26461-y2041-1723https://doaj.org/article/dc4c7d49e7c84512854b815f71269ae52021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26461-yhttps://doaj.org/toc/2041-1723Base editors allow for precision engineering of the genome. Here, the authors present BEAR, a plasmid-based fluorescence assay for the measurement of CBE and ABE activity, to reveal the mechanism underlying their differences and to increase the yield of edited cells with reduced indel background.András TálasDorottya A. SimonPéter I. KulcsárÉva VargaSarah L. KrauszErvin WelkerNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q András Tálas Dorottya A. Simon Péter I. Kulcsár Éva Varga Sarah L. Krausz Ervin Welker BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
description	Base editors allow for precision engineering of the genome. Here, the authors present BEAR, a plasmid-based fluorescence assay for the measurement of CBE and ABE activity, to reveal the mechanism underlying their differences and to increase the yield of edited cells with reduced indel background.
format	article
author	András Tálas Dorottya A. Simon Péter I. Kulcsár Éva Varga Sarah L. Krausz Ervin Welker
author_facet	András Tálas Dorottya A. Simon Péter I. Kulcsár Éva Varga Sarah L. Krausz Ervin Welker
author_sort	András Tálas
title	BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
title_short	BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
title_full	BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
title_fullStr	BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
title_full_unstemmed	BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
title_sort	bear reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dc4c7d49e7c84512854b815f71269ae5
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BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors

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