The Impact of CB1 Receptor on Inflammation in Skeletal Muscle Cells

Mansour Haddad Faculty of Pharmacy, Philadelphia University, Amman, JordanCorrespondence: Mansour Haddad Email Dr.man.haddad@gmail.comBackground: Various factors trigger the inflammatory response and cytokine activation in skeletal muscle. Inflamed muscle will exhibit significant levels of inflammat...

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Autor principal:	Haddad M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	cannabinoid cb1 receptors skeletal muscle interleukin 6 acea inflammation Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/dc562069441f4115b2bde8b1e47df35d
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spelling	oai:doaj.org-article:dc562069441f4115b2bde8b1e47df35d2021-12-02T15:07:50ZThe Impact of CB1 Receptor on Inflammation in Skeletal Muscle Cells1178-7031https://doaj.org/article/dc562069441f4115b2bde8b1e47df35d2021-08-01T00:00:00Zhttps://www.dovepress.com/the-impact-of-cb1-receptor-on-inflammation-in-skeletal-muscle-cells-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Mansour Haddad Faculty of Pharmacy, Philadelphia University, Amman, JordanCorrespondence: Mansour Haddad Email Dr.man.haddad@gmail.comBackground: Various factors trigger the inflammatory response and cytokine activation in skeletal muscle. Inflamed muscle will exhibit significant levels of inflammation and cytokine activity. Interleukin-6 (IL-6), a pro-inflammatory cytokine, exerts pleiotropic effects on skeletal muscle. Endocannabinoid produced by all cell types binds to a class of G protein-coupled receptors, in particular cannabinoid CB1 receptors, to induce skeletal muscle actions.Objective: The purpose of this research was to discover whether activation of cannabinoid CB1 receptors in L6 skeletal muscle cells may promote IL-6 gene expression.Materials and Methods: L6 skeletal muscle cells were cultured in 25 cm2 flasks and quantitative reverse transcription-polymerase chain reaction (probe-based) utilised to quantify IL-6 gene expression levels among different treatment settings.Results: Arachidonyl-2ʹ-chloroethylamide (ACEA) 10 nM, a persistent selective CB1 receptor agonist, promotes IL-6 gene expression in a time-dependent manner. Rimonabant 100 nM, a selective cannabinoid CB1 receptor antagonist, blocks the impact of ACEA. However, insulin does not change IL-6 gene expression.Conclusion: For the first time, a unique link between ACEA and IL-6 up-regulation has been established; IL-6 up-regulation generated by ACEA is mediated in skeletal muscle through cannabinoid CB1 receptor activation. As a result, cannabinoid CB1 receptors may be useful pharmaceutical targets in the treatment of inflammation and related disorders in skeletal muscle tissues.Keywords: cannabinoid CB1 receptors, skeletal muscle, interleukin 6, ACEA, inflammationHaddad MDove Medical Pressarticlecannabinoid cb1 receptorsskeletal muscleinterleukin 6aceainflammationPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 3959-3967 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cannabinoid cb1 receptors skeletal muscle interleukin 6 acea inflammation Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	cannabinoid cb1 receptors skeletal muscle interleukin 6 acea inflammation Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Haddad M The Impact of CB1 Receptor on Inflammation in Skeletal Muscle Cells
description	Mansour Haddad Faculty of Pharmacy, Philadelphia University, Amman, JordanCorrespondence: Mansour Haddad Email Dr.man.haddad@gmail.comBackground: Various factors trigger the inflammatory response and cytokine activation in skeletal muscle. Inflamed muscle will exhibit significant levels of inflammation and cytokine activity. Interleukin-6 (IL-6), a pro-inflammatory cytokine, exerts pleiotropic effects on skeletal muscle. Endocannabinoid produced by all cell types binds to a class of G protein-coupled receptors, in particular cannabinoid CB1 receptors, to induce skeletal muscle actions.Objective: The purpose of this research was to discover whether activation of cannabinoid CB1 receptors in L6 skeletal muscle cells may promote IL-6 gene expression.Materials and Methods: L6 skeletal muscle cells were cultured in 25 cm2 flasks and quantitative reverse transcription-polymerase chain reaction (probe-based) utilised to quantify IL-6 gene expression levels among different treatment settings.Results: Arachidonyl-2ʹ-chloroethylamide (ACEA) 10 nM, a persistent selective CB1 receptor agonist, promotes IL-6 gene expression in a time-dependent manner. Rimonabant 100 nM, a selective cannabinoid CB1 receptor antagonist, blocks the impact of ACEA. However, insulin does not change IL-6 gene expression.Conclusion: For the first time, a unique link between ACEA and IL-6 up-regulation has been established; IL-6 up-regulation generated by ACEA is mediated in skeletal muscle through cannabinoid CB1 receptor activation. As a result, cannabinoid CB1 receptors may be useful pharmaceutical targets in the treatment of inflammation and related disorders in skeletal muscle tissues.Keywords: cannabinoid CB1 receptors, skeletal muscle, interleukin 6, ACEA, inflammation
format	article
author	Haddad M
author_facet	Haddad M
author_sort	Haddad M
title	The Impact of CB1 Receptor on Inflammation in Skeletal Muscle Cells
title_short	The Impact of CB1 Receptor on Inflammation in Skeletal Muscle Cells
title_full	The Impact of CB1 Receptor on Inflammation in Skeletal Muscle Cells
title_fullStr	The Impact of CB1 Receptor on Inflammation in Skeletal Muscle Cells
title_full_unstemmed	The Impact of CB1 Receptor on Inflammation in Skeletal Muscle Cells
title_sort	impact of cb1 receptor on inflammation in skeletal muscle cells
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/dc562069441f4115b2bde8b1e47df35d
work_keys_str_mv	AT haddadm theimpactofcb1receptoroninflammationinskeletalmusclecells AT haddadm impactofcb1receptoroninflammationinskeletalmusclecells
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The Impact of CB1 Receptor on Inflammation in Skeletal Muscle Cells

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