Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic

Abstract Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N2) fixing marine diazotrophs, knowledge gaps...

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Autores principales:	Seaver Wang, Weiyi Tang, Erwan Delage, Scott Gifford, Hannah Whitby, Aridane G. González, Damien Eveillard, Hélène Planquette, Nicolas Cassar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc5f266fd8a446bd9827eb33cf1b8eaa
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spelling	oai:doaj.org-article:dc5f266fd8a446bd9827eb33cf1b8eaa2021-12-02T13:20:03ZInvestigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic10.1038/s41598-021-84969-12045-2322https://doaj.org/article/dc5f266fd8a446bd9827eb33cf1b8eaa2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84969-1https://doaj.org/toc/2045-2322Abstract Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N2) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N2 fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N2 fixation and net community production (NCP). Maximum N2 fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae. Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N2 fixation coastal sites.Seaver WangWeiyi TangErwan DelageScott GiffordHannah WhitbyAridane G. GonzálezDamien EveillardHélène PlanquetteNicolas CassarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seaver Wang Weiyi Tang Erwan Delage Scott Gifford Hannah Whitby Aridane G. González Damien Eveillard Hélène Planquette Nicolas Cassar Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic
description	Abstract Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N2) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N2 fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N2 fixation and net community production (NCP). Maximum N2 fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae. Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N2 fixation coastal sites.
format	article
author	Seaver Wang Weiyi Tang Erwan Delage Scott Gifford Hannah Whitby Aridane G. González Damien Eveillard Hélène Planquette Nicolas Cassar
author_facet	Seaver Wang Weiyi Tang Erwan Delage Scott Gifford Hannah Whitby Aridane G. González Damien Eveillard Hélène Planquette Nicolas Cassar
author_sort	Seaver Wang
title	Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic
title_short	Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic
title_full	Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic
title_fullStr	Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic
title_full_unstemmed	Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic
title_sort	investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western north atlantic
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dc5f266fd8a446bd9827eb33cf1b8eaa
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Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic

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