Identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis

Abstract Olfaction and gustation play critical roles during the host-location search process of insects. Several chemosensory receptor genes are thought to be involved in providing specificity to the olfactory sensory neuron responses. The aphid endoparasitoid, Aphidius gifuensis, has been used as a...

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Autores principales:	Zhi-Wei Kang, Hong-Gang Tian, Fang-Hua Liu, Xiang Liu, Xiang-Feng Jing, Tong-Xian Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc61bb1c524740ec8239fc74b95fd3c8
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spelling	oai:doaj.org-article:dc61bb1c524740ec8239fc74b95fd3c82021-12-02T12:32:13ZIdentification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis10.1038/s41598-017-03988-z2045-2322https://doaj.org/article/dc61bb1c524740ec8239fc74b95fd3c82017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03988-zhttps://doaj.org/toc/2045-2322Abstract Olfaction and gustation play critical roles during the host-location search process of insects. Several chemosensory receptor genes are thought to be involved in providing specificity to the olfactory sensory neuron responses. The aphid endoparasitoid, Aphidius gifuensis, has been used as a biological control agent against a variety of aphid species; this parasitoid is able to detect its target host(s) effectively during the parasitic process. To understand the mechanism of host detection in A. gifuensis, we assembled specific antennal transcriptomes of each sex through next generation sequencing technology to identify the major chemosensory receptor genes. Using a bioinformatics screen, we identified 100 olfactory receptors candidates (62 odorant receptors, 15 gustatory receptors, and 23 ionotropic receptors) from the sex-specific antennal transcriptome. In addition, combining with the demonstrated functions of chemosensory genes in other insects, the sex-, tissue-, and host-specific expression profile of chemosensory genes potentially revealed the candidate physiological functions. The identification and expression profile of chemosensory receptor genes in A. gifuensis provide valuable information for understanding and investigating the intraspecific or interspecific chemical communications in the solitary parasitic wasps.Zhi-Wei KangHong-Gang TianFang-Hua LiuXiang LiuXiang-Feng JingTong-Xian LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhi-Wei Kang Hong-Gang Tian Fang-Hua Liu Xiang Liu Xiang-Feng Jing Tong-Xian Liu Identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis
description	Abstract Olfaction and gustation play critical roles during the host-location search process of insects. Several chemosensory receptor genes are thought to be involved in providing specificity to the olfactory sensory neuron responses. The aphid endoparasitoid, Aphidius gifuensis, has been used as a biological control agent against a variety of aphid species; this parasitoid is able to detect its target host(s) effectively during the parasitic process. To understand the mechanism of host detection in A. gifuensis, we assembled specific antennal transcriptomes of each sex through next generation sequencing technology to identify the major chemosensory receptor genes. Using a bioinformatics screen, we identified 100 olfactory receptors candidates (62 odorant receptors, 15 gustatory receptors, and 23 ionotropic receptors) from the sex-specific antennal transcriptome. In addition, combining with the demonstrated functions of chemosensory genes in other insects, the sex-, tissue-, and host-specific expression profile of chemosensory genes potentially revealed the candidate physiological functions. The identification and expression profile of chemosensory receptor genes in A. gifuensis provide valuable information for understanding and investigating the intraspecific or interspecific chemical communications in the solitary parasitic wasps.
format	article
author	Zhi-Wei Kang Hong-Gang Tian Fang-Hua Liu Xiang Liu Xiang-Feng Jing Tong-Xian Liu
author_facet	Zhi-Wei Kang Hong-Gang Tian Fang-Hua Liu Xiang Liu Xiang-Feng Jing Tong-Xian Liu
author_sort	Zhi-Wei Kang
title	Identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis
title_short	Identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis
title_full	Identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis
title_fullStr	Identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis
title_full_unstemmed	Identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis
title_sort	identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid aphidius gifuensis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/dc61bb1c524740ec8239fc74b95fd3c8
work_keys_str_mv	AT zhiweikang identificationandexpressionanalysisofchemosensoryreceptorgenesinanaphidendoparasitoidaphidiusgifuensis AT honggangtian identificationandexpressionanalysisofchemosensoryreceptorgenesinanaphidendoparasitoidaphidiusgifuensis AT fanghualiu identificationandexpressionanalysisofchemosensoryreceptorgenesinanaphidendoparasitoidaphidiusgifuensis AT xiangliu identificationandexpressionanalysisofchemosensoryreceptorgenesinanaphidendoparasitoidaphidiusgifuensis AT xiangfengjing identificationandexpressionanalysisofchemosensoryreceptorgenesinanaphidendoparasitoidaphidiusgifuensis AT tongxianliu identificationandexpressionanalysisofchemosensoryreceptorgenesinanaphidendoparasitoidaphidiusgifuensis
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Identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis

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