Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development...

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Autores principales: Alicia Lundby, Hiroki Mutoh, Dimitar Dimitrov, Walther Akemann, Thomas Knöpfel
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2008
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Acceso en línea:https://doaj.org/article/dc6d0049eba54cd5aca726cf3bc19690
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Sumario:Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.