Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development...

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Auteurs principaux:	Alicia Lundby, Hiroki Mutoh, Dimitar Dimitrov, Walther Akemann, Thomas Knöpfel
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2008
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/dc6d0049eba54cd5aca726cf3bc19690
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spelling	oai:doaj.org-article:dc6d0049eba54cd5aca726cf3bc196902021-11-25T06:11:53ZEngineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.1932-620310.1371/journal.pone.0002514https://doaj.org/article/dc6d0049eba54cd5aca726cf3bc196902008-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18575613/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.Alicia LundbyHiroki MutohDimitar DimitrovWalther AkemannThomas KnöpfelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 6, p e2514 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alicia Lundby Hiroki Mutoh Dimitar Dimitrov Walther Akemann Thomas Knöpfel Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
description	Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.
format	article
author	Alicia Lundby Hiroki Mutoh Dimitar Dimitrov Walther Akemann Thomas Knöpfel
author_facet	Alicia Lundby Hiroki Mutoh Dimitar Dimitrov Walther Akemann Thomas Knöpfel
author_sort	Alicia Lundby
title	Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_short	Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_full	Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_fullStr	Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_full_unstemmed	Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_sort	engineering of a genetically encodable fluorescent voltage sensor exploiting fast ci-vsp voltage-sensing movements.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/dc6d0049eba54cd5aca726cf3bc19690
work_keys_str_mv	AT alicialundby engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements AT hirokimutoh engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements AT dimitardimitrov engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements AT waltherakemann engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements AT thomasknopfel engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements
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Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.

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