Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development...

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Autores principales: Alicia Lundby, Hiroki Mutoh, Dimitar Dimitrov, Walther Akemann, Thomas Knöpfel
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Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2008
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Acceso en línea:https://doaj.org/article/dc6d0049eba54cd5aca726cf3bc19690
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spelling oai:doaj.org-article:dc6d0049eba54cd5aca726cf3bc196902021-11-25T06:11:53ZEngineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.1932-620310.1371/journal.pone.0002514https://doaj.org/article/dc6d0049eba54cd5aca726cf3bc196902008-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18575613/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.Alicia LundbyHiroki MutohDimitar DimitrovWalther AkemannThomas KnöpfelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 6, p e2514 (2008)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Alicia Lundby
Hiroki Mutoh
Dimitar Dimitrov
Walther Akemann
Thomas Knöpfel
Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
description Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.
format article
author Alicia Lundby
Hiroki Mutoh
Dimitar Dimitrov
Walther Akemann
Thomas Knöpfel
author_facet Alicia Lundby
Hiroki Mutoh
Dimitar Dimitrov
Walther Akemann
Thomas Knöpfel
author_sort Alicia Lundby
title Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_short Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_full Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_fullStr Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_full_unstemmed Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
title_sort engineering of a genetically encodable fluorescent voltage sensor exploiting fast ci-vsp voltage-sensing movements.
publisher Public Library of Science (PLoS)
publishDate 2008
url https://doaj.org/article/dc6d0049eba54cd5aca726cf3bc19690
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AT dimitardimitrov engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements
AT waltherakemann engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements
AT thomasknopfel engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements
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