Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development...
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Public Library of Science (PLoS)
2008
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oai:doaj.org-article:dc6d0049eba54cd5aca726cf3bc196902021-11-25T06:11:53ZEngineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.1932-620310.1371/journal.pone.0002514https://doaj.org/article/dc6d0049eba54cd5aca726cf3bc196902008-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18575613/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.Alicia LundbyHiroki MutohDimitar DimitrovWalther AkemannThomas KnöpfelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 6, p e2514 (2008) |
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Medicine R Science Q Alicia Lundby Hiroki Mutoh Dimitar Dimitrov Walther Akemann Thomas Knöpfel Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. |
description |
Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs. |
format |
article |
author |
Alicia Lundby Hiroki Mutoh Dimitar Dimitrov Walther Akemann Thomas Knöpfel |
author_facet |
Alicia Lundby Hiroki Mutoh Dimitar Dimitrov Walther Akemann Thomas Knöpfel |
author_sort |
Alicia Lundby |
title |
Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. |
title_short |
Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. |
title_full |
Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. |
title_fullStr |
Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. |
title_full_unstemmed |
Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. |
title_sort |
engineering of a genetically encodable fluorescent voltage sensor exploiting fast ci-vsp voltage-sensing movements. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2008 |
url |
https://doaj.org/article/dc6d0049eba54cd5aca726cf3bc19690 |
work_keys_str_mv |
AT alicialundby engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements AT hirokimutoh engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements AT dimitardimitrov engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements AT waltherakemann engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements AT thomasknopfel engineeringofageneticallyencodablefluorescentvoltagesensorexploitingfastcivspvoltagesensingmovements |
_version_ |
1718414074217234432 |