Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age

Abstract Neonatal calves possess a very immature and naïve immune system and are reliant on the intake of maternal colostrum for passive transfer of immunoglobulins. Variation in colostrum management of beef and dairy calves is thought to affect early immune development. Therefore, the objective of...

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Autores principales:	C. Surlis, B. Earley, M. McGee, K. Keogh, P. Cormican, G. Blackshields, K. Tiernan, A. Dunn, S. Morrison, A. Arguello, S. M. Waters
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Dairy Calves Beef Calves Beef Suckler Colostrum Management Differentially Expressed Genes (DEG) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc6e8f152c67440d8d4140385216ed88
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spelling	oai:doaj.org-article:dc6e8f152c67440d8d4140385216ed882021-12-02T15:08:12ZBlood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age10.1038/s41598-018-33627-02045-2322https://doaj.org/article/dc6e8f152c67440d8d4140385216ed882018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33627-0https://doaj.org/toc/2045-2322Abstract Neonatal calves possess a very immature and naïve immune system and are reliant on the intake of maternal colostrum for passive transfer of immunoglobulins. Variation in colostrum management of beef and dairy calves is thought to affect early immune development. Therefore, the objective of this study was to examine changes in gene expression and investigate molecular pathways involved in the immune-competence development of neonatal Holstein dairy calves and naturally suckled beef calves using next generation RNA-sequencing during the first week of life. Jugular whole blood samples were collected from Holstein (H) dairy calves (n = 8) artificially fed 5% B.W. colostrum, and from beef calves which were the progenies of Charolais-Limousin (CL; n = 7) and Limousin-Friesian beef suckler cows (LF; n = 7), for subsequent RNA isolation. In dairy calves, there was a surge in pro-inflammatory cytokine gene expression possibly due to the stress of separation from the dam. LF calves exhibited early signs of humoral immune development with observed increases in the expression genes coding for Ig receptors, which was not evident in the other breeds by 7 days of age. Immune and health related DEGs identified as upregulated in beef calves are prospective contender genes for the classification of biomarkers for immune-competence development, and will contribute towards a greater understanding of the development of an immune response in neonatal calves.C. SurlisB. EarleyM. McGeeK. KeoghP. CormicanG. BlackshieldsK. TiernanA. DunnS. MorrisonA. ArguelloS. M. WatersNature PortfolioarticleDairy CalvesBeef CalvesBeef SucklerColostrum ManagementDifferentially Expressed Genes (DEG)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dairy Calves Beef Calves Beef Suckler Colostrum Management Differentially Expressed Genes (DEG) Medicine R Science Q
spellingShingle	Dairy Calves Beef Calves Beef Suckler Colostrum Management Differentially Expressed Genes (DEG) Medicine R Science Q C. Surlis B. Earley M. McGee K. Keogh P. Cormican G. Blackshields K. Tiernan A. Dunn S. Morrison A. Arguello S. M. Waters Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age
description	Abstract Neonatal calves possess a very immature and naïve immune system and are reliant on the intake of maternal colostrum for passive transfer of immunoglobulins. Variation in colostrum management of beef and dairy calves is thought to affect early immune development. Therefore, the objective of this study was to examine changes in gene expression and investigate molecular pathways involved in the immune-competence development of neonatal Holstein dairy calves and naturally suckled beef calves using next generation RNA-sequencing during the first week of life. Jugular whole blood samples were collected from Holstein (H) dairy calves (n = 8) artificially fed 5% B.W. colostrum, and from beef calves which were the progenies of Charolais-Limousin (CL; n = 7) and Limousin-Friesian beef suckler cows (LF; n = 7), for subsequent RNA isolation. In dairy calves, there was a surge in pro-inflammatory cytokine gene expression possibly due to the stress of separation from the dam. LF calves exhibited early signs of humoral immune development with observed increases in the expression genes coding for Ig receptors, which was not evident in the other breeds by 7 days of age. Immune and health related DEGs identified as upregulated in beef calves are prospective contender genes for the classification of biomarkers for immune-competence development, and will contribute towards a greater understanding of the development of an immune response in neonatal calves.
format	article
author	C. Surlis B. Earley M. McGee K. Keogh P. Cormican G. Blackshields K. Tiernan A. Dunn S. Morrison A. Arguello S. M. Waters
author_facet	C. Surlis B. Earley M. McGee K. Keogh P. Cormican G. Blackshields K. Tiernan A. Dunn S. Morrison A. Arguello S. M. Waters
author_sort	C. Surlis
title	Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age
title_short	Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age
title_full	Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age
title_fullStr	Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age
title_full_unstemmed	Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age
title_sort	blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/dc6e8f152c67440d8d4140385216ed88
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Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age

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