Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.

Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression of foreign proteins may induce immu...

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Autores principales:	Lei Huang, Ramireddy Bommireddy, Luis E Munoz, Rohini N Guin, Changyong Wei, Amanda Ruggieri, Ashwathi P Menon, Xiaoxian Li, Mala Shanmugam, Taofeek K Owonikoko, Suresh S Ramalingam, Periasamy Selvaraj
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc6e930ff2a44d15a2ac8e0dd7a119d8
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spelling	oai:doaj.org-article:dc6e930ff2a44d15a2ac8e0dd7a119d82021-12-02T20:14:58ZExpression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.1932-620310.1371/journal.pone.0254125https://doaj.org/article/dc6e930ff2a44d15a2ac8e0dd7a119d82021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254125https://doaj.org/toc/1932-6203Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression of foreign proteins may induce immune responses in immunocompetent syngeneic tumor transplant models and augment the efficacy of experimental drugs. In this study, we show that the growth rate of Lewis lung carcinoma (LL/2) tumors was reduced after transduction of tdTomato and luciferase (tdTomato/Luc) compared to the parental cell line. tdTomato/Luc expression by LL/2 cells altered the tumor microenvironment by increasing tumor-infiltrating lymphocytes (TILs) while inhibiting tumor-induced myeloid-derived suppressor cells (MDSCs). Interestingly, tdTomato/Luc expression did not alter the response of LL/2 tumors to anti-PD-1 and anti-CTLA-4 antibodies. These results suggest that the use of tdTomato/Luc-transduced cancer cells to conduct studies in immune competent mice may lead to cell-extrinsic tdTomato/Luc-induced alterations in tumor growth and tumor immune microenvironment that need to be taken into consideration when evaluating the efficacy of anti-cancer drugs and vaccines in immunocompetent animal models.Lei HuangRamireddy BommireddyLuis E MunozRohini N GuinChangyong WeiAmanda RuggieriAshwathi P MenonXiaoxian LiMala ShanmugamTaofeek K OwonikokoSuresh S RamalingamPeriasamy SelvarajPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0254125 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lei Huang Ramireddy Bommireddy Luis E Munoz Rohini N Guin Changyong Wei Amanda Ruggieri Ashwathi P Menon Xiaoxian Li Mala Shanmugam Taofeek K Owonikoko Suresh S Ramalingam Periasamy Selvaraj Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.
description	Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression of foreign proteins may induce immune responses in immunocompetent syngeneic tumor transplant models and augment the efficacy of experimental drugs. In this study, we show that the growth rate of Lewis lung carcinoma (LL/2) tumors was reduced after transduction of tdTomato and luciferase (tdTomato/Luc) compared to the parental cell line. tdTomato/Luc expression by LL/2 cells altered the tumor microenvironment by increasing tumor-infiltrating lymphocytes (TILs) while inhibiting tumor-induced myeloid-derived suppressor cells (MDSCs). Interestingly, tdTomato/Luc expression did not alter the response of LL/2 tumors to anti-PD-1 and anti-CTLA-4 antibodies. These results suggest that the use of tdTomato/Luc-transduced cancer cells to conduct studies in immune competent mice may lead to cell-extrinsic tdTomato/Luc-induced alterations in tumor growth and tumor immune microenvironment that need to be taken into consideration when evaluating the efficacy of anti-cancer drugs and vaccines in immunocompetent animal models.
format	article
author	Lei Huang Ramireddy Bommireddy Luis E Munoz Rohini N Guin Changyong Wei Amanda Ruggieri Ashwathi P Menon Xiaoxian Li Mala Shanmugam Taofeek K Owonikoko Suresh S Ramalingam Periasamy Selvaraj
author_facet	Lei Huang Ramireddy Bommireddy Luis E Munoz Rohini N Guin Changyong Wei Amanda Ruggieri Ashwathi P Menon Xiaoxian Li Mala Shanmugam Taofeek K Owonikoko Suresh S Ramalingam Periasamy Selvaraj
author_sort	Lei Huang
title	Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.
title_short	Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.
title_full	Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.
title_fullStr	Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.
title_full_unstemmed	Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.
title_sort	expression of tdtomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/dc6e930ff2a44d15a2ac8e0dd7a119d8
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Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.

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