Disease Severity, Resistance Analysis, and Expression Profiling of Pathogenesis-Related Protein Genes after the Inoculation of <i>Fusarium equiseti</i> in Wheat

Wheat (<i>Triticum aestivum</i>) is an important cereal crop, grown throughout the temperate and in some tropical and sub-tropical zones, at higher elevations. Several biotic and abiotic factors influence the production of wheat. In the present study, two wheat varieties have been subjec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hakim Manghwar, Amjad Hussain, Qurban Ali, Muhammad Hamzah Saleem, Muyassar H. Abualreesh, Aishah Alatawi, Shafaqat Ali, Muhammad Farooq Hussain Munis
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plant defense mechanism plant disease pathogenicity <i>Triticum aesitivum</i> Shafaq-2006 Sahar-2006 Agriculture S
Acceso en línea:	https://doaj.org/article/dc73419916af4c828189b8ae7b388ffe
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dc73419916af4c828189b8ae7b388ffe
record_format	dspace
spelling	oai:doaj.org-article:dc73419916af4c828189b8ae7b388ffe2021-11-25T16:03:30ZDisease Severity, Resistance Analysis, and Expression Profiling of Pathogenesis-Related Protein Genes after the Inoculation of <i>Fusarium equiseti</i> in Wheat10.3390/agronomy111121242073-4395https://doaj.org/article/dc73419916af4c828189b8ae7b388ffe2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2124https://doaj.org/toc/2073-4395Wheat (<i>Triticum aestivum</i>) is an important cereal crop, grown throughout the temperate and in some tropical and sub-tropical zones, at higher elevations. Several biotic and abiotic factors influence the production of wheat. In the present study, two wheat varieties have been subjected to disease severity and resistance analysis against <i>Fusarium equiseti</i>. Disease severity analysis revealed Shafaq-2006 to be more resistant than Sahar-2006. Both varieties were further subjected to the expression analysis of six important defense-related genes by RT-PCR and quantitative real-time PCR. This analysis revealed that PR-1, TLP, Chitinase, and β-1,3-glucanase genes were highly expressed in Shafaq-2006 and possibly play a significant role in its defense mechanism. In addition, biochemical and physiochemical parameters were also studied to further explore the difference between resistant and susceptible varieties. With total proline and protein contents, sugar and chlorophyll contents also increased significantly in resistant variety. Likewise, higher relative water content, total plant length, and the high root–shoot ratio was observed in resistant plants, compared to susceptible wheat plants. These increases in chemical and physiological parameters might be related to the activation of the defense mechanism due to the higher expression of PR genes in the resistant wheat variety. These genes can further be employed for cloning into wheat and other transgenic crops to develop resistance against <i>F. equiseti</i>.Hakim ManghwarAmjad HussainQurban AliMuhammad Hamzah SaleemMuyassar H. AbualreeshAishah AlatawiShafaqat AliMuhammad Farooq Hussain MunisMDPI AGarticleplant defense mechanismplant diseasepathogenicity<i>Triticum aesitivum</i>Shafaq-2006Sahar-2006AgricultureSENAgronomy, Vol 11, Iss 2124, p 2124 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant defense mechanism plant disease pathogenicity <i>Triticum aesitivum</i> Shafaq-2006 Sahar-2006 Agriculture S
spellingShingle	plant defense mechanism plant disease pathogenicity <i>Triticum aesitivum</i> Shafaq-2006 Sahar-2006 Agriculture S Hakim Manghwar Amjad Hussain Qurban Ali Muhammad Hamzah Saleem Muyassar H. Abualreesh Aishah Alatawi Shafaqat Ali Muhammad Farooq Hussain Munis Disease Severity, Resistance Analysis, and Expression Profiling of Pathogenesis-Related Protein Genes after the Inoculation of <i>Fusarium equiseti</i> in Wheat
description	Wheat (<i>Triticum aestivum</i>) is an important cereal crop, grown throughout the temperate and in some tropical and sub-tropical zones, at higher elevations. Several biotic and abiotic factors influence the production of wheat. In the present study, two wheat varieties have been subjected to disease severity and resistance analysis against <i>Fusarium equiseti</i>. Disease severity analysis revealed Shafaq-2006 to be more resistant than Sahar-2006. Both varieties were further subjected to the expression analysis of six important defense-related genes by RT-PCR and quantitative real-time PCR. This analysis revealed that PR-1, TLP, Chitinase, and β-1,3-glucanase genes were highly expressed in Shafaq-2006 and possibly play a significant role in its defense mechanism. In addition, biochemical and physiochemical parameters were also studied to further explore the difference between resistant and susceptible varieties. With total proline and protein contents, sugar and chlorophyll contents also increased significantly in resistant variety. Likewise, higher relative water content, total plant length, and the high root–shoot ratio was observed in resistant plants, compared to susceptible wheat plants. These increases in chemical and physiological parameters might be related to the activation of the defense mechanism due to the higher expression of PR genes in the resistant wheat variety. These genes can further be employed for cloning into wheat and other transgenic crops to develop resistance against <i>F. equiseti</i>.
format	article
author	Hakim Manghwar Amjad Hussain Qurban Ali Muhammad Hamzah Saleem Muyassar H. Abualreesh Aishah Alatawi Shafaqat Ali Muhammad Farooq Hussain Munis
author_facet	Hakim Manghwar Amjad Hussain Qurban Ali Muhammad Hamzah Saleem Muyassar H. Abualreesh Aishah Alatawi Shafaqat Ali Muhammad Farooq Hussain Munis
author_sort	Hakim Manghwar
title	Disease Severity, Resistance Analysis, and Expression Profiling of Pathogenesis-Related Protein Genes after the Inoculation of <i>Fusarium equiseti</i> in Wheat
title_short	Disease Severity, Resistance Analysis, and Expression Profiling of Pathogenesis-Related Protein Genes after the Inoculation of <i>Fusarium equiseti</i> in Wheat
title_full	Disease Severity, Resistance Analysis, and Expression Profiling of Pathogenesis-Related Protein Genes after the Inoculation of <i>Fusarium equiseti</i> in Wheat
title_fullStr	Disease Severity, Resistance Analysis, and Expression Profiling of Pathogenesis-Related Protein Genes after the Inoculation of <i>Fusarium equiseti</i> in Wheat
title_full_unstemmed	Disease Severity, Resistance Analysis, and Expression Profiling of Pathogenesis-Related Protein Genes after the Inoculation of <i>Fusarium equiseti</i> in Wheat
title_sort	disease severity, resistance analysis, and expression profiling of pathogenesis-related protein genes after the inoculation of <i>fusarium equiseti</i> in wheat
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dc73419916af4c828189b8ae7b388ffe
work_keys_str_mv	AT hakimmanghwar diseaseseverityresistanceanalysisandexpressionprofilingofpathogenesisrelatedproteingenesaftertheinoculationofifusariumequisetiiinwheat AT amjadhussain diseaseseverityresistanceanalysisandexpressionprofilingofpathogenesisrelatedproteingenesaftertheinoculationofifusariumequisetiiinwheat AT qurbanali diseaseseverityresistanceanalysisandexpressionprofilingofpathogenesisrelatedproteingenesaftertheinoculationofifusariumequisetiiinwheat AT muhammadhamzahsaleem diseaseseverityresistanceanalysisandexpressionprofilingofpathogenesisrelatedproteingenesaftertheinoculationofifusariumequisetiiinwheat AT muyassarhabualreesh diseaseseverityresistanceanalysisandexpressionprofilingofpathogenesisrelatedproteingenesaftertheinoculationofifusariumequisetiiinwheat AT aishahalatawi diseaseseverityresistanceanalysisandexpressionprofilingofpathogenesisrelatedproteingenesaftertheinoculationofifusariumequisetiiinwheat AT shafaqatali diseaseseverityresistanceanalysisandexpressionprofilingofpathogenesisrelatedproteingenesaftertheinoculationofifusariumequisetiiinwheat AT muhammadfarooqhussainmunis diseaseseverityresistanceanalysisandexpressionprofilingofpathogenesisrelatedproteingenesaftertheinoculationofifusariumequisetiiinwheat
_version_	1718413346854666240

Disease Severity, Resistance Analysis, and Expression Profiling of Pathogenesis-Related Protein Genes after the Inoculation of <i>Fusarium equiseti</i> in Wheat

Ejemplares similares