Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome

Abstract High-protein diets often lead to an increase in urea concentration in follicular fluid of dairy cows, which may reduce oocyte competence. In the present study, maturation media were supplemented with urea (0, 20, 40 mg/dl), and amino acids (AAs) turnover was evaluated in the 24-h spent medi...

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Autores principales:	Rasoul Kowsar, Vahid Norozian Iranshahi, Nima Sadeghi, Ahmad Riasi, Akio Miyamoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc852ce29d034b079266833ff580e82a
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spelling	oai:doaj.org-article:dc852ce29d034b079266833ff580e82a2021-12-02T15:08:26ZUrea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome10.1038/s41598-018-30774-22045-2322https://doaj.org/article/dc852ce29d034b079266833ff580e82a2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30774-2https://doaj.org/toc/2045-2322Abstract High-protein diets often lead to an increase in urea concentration in follicular fluid of dairy cows, which may reduce oocyte competence. In the present study, maturation media were supplemented with urea (0, 20, 40 mg/dl), and amino acids (AAs) turnover was evaluated in the 24-h spent media of specimens (cell types), bovine cumulus-oocyte complexes (COCs), cumulus cells (CCs), or denuded oocytes (DOs). The main effects of urea and cell type, and their interaction were significant on the individual turnover (expect threonine, glycine, and tyrosine) and total turnover, depletion, and appearance of AAs. The results showed a high level of urea and DOs increased the depletion of all AAs and that of essential and non-AAs, respectively. Sensitivity analysis revealed the highest sensitivity of isoleucine, lysine, and tryptophan to urea, especially in DOs. Principal component analysis (PCA) evaluated the strong correlations between the turnover of: (1) glutamine, aspartic acid or glycine, and developmental competence and fertilization of COCs; (2) serine, isoleucine, valine or glutamic acid, and cleavage rate of DOs; and (3) serine, glutamine, aspartic acid or alanine, and CCs viability. In conclusion, urea significantly changed the turnover of AAs by COCs, CCs and DOs, and reduced the subsequent developmental competence of bovine oocytes.Rasoul KowsarVahid Norozian IranshahiNima SadeghiAhmad RiasiAkio MiyamotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rasoul Kowsar Vahid Norozian Iranshahi Nima Sadeghi Ahmad Riasi Akio Miyamoto Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome
description	Abstract High-protein diets often lead to an increase in urea concentration in follicular fluid of dairy cows, which may reduce oocyte competence. In the present study, maturation media were supplemented with urea (0, 20, 40 mg/dl), and amino acids (AAs) turnover was evaluated in the 24-h spent media of specimens (cell types), bovine cumulus-oocyte complexes (COCs), cumulus cells (CCs), or denuded oocytes (DOs). The main effects of urea and cell type, and their interaction were significant on the individual turnover (expect threonine, glycine, and tyrosine) and total turnover, depletion, and appearance of AAs. The results showed a high level of urea and DOs increased the depletion of all AAs and that of essential and non-AAs, respectively. Sensitivity analysis revealed the highest sensitivity of isoleucine, lysine, and tryptophan to urea, especially in DOs. Principal component analysis (PCA) evaluated the strong correlations between the turnover of: (1) glutamine, aspartic acid or glycine, and developmental competence and fertilization of COCs; (2) serine, isoleucine, valine or glutamic acid, and cleavage rate of DOs; and (3) serine, glutamine, aspartic acid or alanine, and CCs viability. In conclusion, urea significantly changed the turnover of AAs by COCs, CCs and DOs, and reduced the subsequent developmental competence of bovine oocytes.
format	article
author	Rasoul Kowsar Vahid Norozian Iranshahi Nima Sadeghi Ahmad Riasi Akio Miyamoto
author_facet	Rasoul Kowsar Vahid Norozian Iranshahi Nima Sadeghi Ahmad Riasi Akio Miyamoto
author_sort	Rasoul Kowsar
title	Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome
title_short	Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome
title_full	Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome
title_fullStr	Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome
title_full_unstemmed	Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome
title_sort	urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/dc852ce29d034b079266833ff580e82a
work_keys_str_mv	AT rasoulkowsar ureainfluencesaminoacidturnoverinbovinecumulusoocytecomplexescumuluscellsanddenudedoocytesandaffectsinvitrofertilizationoutcome AT vahidnorozianiranshahi ureainfluencesaminoacidturnoverinbovinecumulusoocytecomplexescumuluscellsanddenudedoocytesandaffectsinvitrofertilizationoutcome AT nimasadeghi ureainfluencesaminoacidturnoverinbovinecumulusoocytecomplexescumuluscellsanddenudedoocytesandaffectsinvitrofertilizationoutcome AT ahmadriasi ureainfluencesaminoacidturnoverinbovinecumulusoocytecomplexescumuluscellsanddenudedoocytesandaffectsinvitrofertilizationoutcome AT akiomiyamoto ureainfluencesaminoacidturnoverinbovinecumulusoocytecomplexescumuluscellsanddenudedoocytesandaffectsinvitrofertilizationoutcome
_version_	1718388154201800704

Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome

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