Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines

Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases cN-II and CD73, together with RNA sequencing a...

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Autores principales: Octavia Cadassou, Prescillia Forey, Christelle Machon, Edoardo Petrotto, Kamel Chettab, Maria Grazia Tozzi, Jérôme Guitton, Charles Dumontet, Emeline Cros-Perrial, Lars Petter Jordheim
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Publicado: MDPI AG 2021
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Acceso en línea:https://doaj.org/article/dc853e9872e7486094c5d65cafe12194
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spelling oai:doaj.org-article:dc853e9872e7486094c5d65cafe121942021-11-25T17:08:53ZTranscriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines10.3390/cells101129182073-4409https://doaj.org/article/dc853e9872e7486094c5d65cafe121942021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2918https://doaj.org/toc/2073-4409Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases cN-II and CD73, together with RNA sequencing and targeted metabolomics, to decipher new regulatory roles of these proteins. We observed important transcriptional modifications between models as well as upon exposure to adenosine. Metabolite content varied differently between cell models in response to adenosine exposure but was rather similar in control conditions. Our original cell models allowed us to identify a new unobvious link between proteins in the nucleotide metabolism and other cellular pathways. Further analyses of our models, including additional experiments, could help us to better understand some of the roles played by these enzymes.Octavia CadassouPrescillia ForeyChristelle MachonEdoardo PetrottoKamel ChettabMaria Grazia TozziJérôme GuittonCharles DumontetEmeline Cros-PerrialLars Petter JordheimMDPI AGarticleCD73cN-IImetabolic pathwaystranscriptional regulationBiology (General)QH301-705.5ENCells, Vol 10, Iss 2918, p 2918 (2021)
institution DOAJ
collection DOAJ
language EN
topic CD73
cN-II
metabolic pathways
transcriptional regulation
Biology (General)
QH301-705.5
spellingShingle CD73
cN-II
metabolic pathways
transcriptional regulation
Biology (General)
QH301-705.5
Octavia Cadassou
Prescillia Forey
Christelle Machon
Edoardo Petrotto
Kamel Chettab
Maria Grazia Tozzi
Jérôme Guitton
Charles Dumontet
Emeline Cros-Perrial
Lars Petter Jordheim
Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
description Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases cN-II and CD73, together with RNA sequencing and targeted metabolomics, to decipher new regulatory roles of these proteins. We observed important transcriptional modifications between models as well as upon exposure to adenosine. Metabolite content varied differently between cell models in response to adenosine exposure but was rather similar in control conditions. Our original cell models allowed us to identify a new unobvious link between proteins in the nucleotide metabolism and other cellular pathways. Further analyses of our models, including additional experiments, could help us to better understand some of the roles played by these enzymes.
format article
author Octavia Cadassou
Prescillia Forey
Christelle Machon
Edoardo Petrotto
Kamel Chettab
Maria Grazia Tozzi
Jérôme Guitton
Charles Dumontet
Emeline Cros-Perrial
Lars Petter Jordheim
author_facet Octavia Cadassou
Prescillia Forey
Christelle Machon
Edoardo Petrotto
Kamel Chettab
Maria Grazia Tozzi
Jérôme Guitton
Charles Dumontet
Emeline Cros-Perrial
Lars Petter Jordheim
author_sort Octavia Cadassou
title Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_short Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_full Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_fullStr Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_full_unstemmed Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_sort transcriptional and metabolic investigation in 5′-nucleotidase deficient cancer cell lines
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/dc853e9872e7486094c5d65cafe12194
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