Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines

Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases cN-II and CD73, together with RNA sequencing a...

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Autores principales:	Octavia Cadassou, Prescillia Forey, Christelle Machon, Edoardo Petrotto, Kamel Chettab, Maria Grazia Tozzi, Jérôme Guitton, Charles Dumontet, Emeline Cros-Perrial, Lars Petter Jordheim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	CD73 cN-II metabolic pathways transcriptional regulation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dc853e9872e7486094c5d65cafe12194
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spelling	oai:doaj.org-article:dc853e9872e7486094c5d65cafe121942021-11-25T17:08:53ZTranscriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines10.3390/cells101129182073-4409https://doaj.org/article/dc853e9872e7486094c5d65cafe121942021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2918https://doaj.org/toc/2073-4409Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases cN-II and CD73, together with RNA sequencing and targeted metabolomics, to decipher new regulatory roles of these proteins. We observed important transcriptional modifications between models as well as upon exposure to adenosine. Metabolite content varied differently between cell models in response to adenosine exposure but was rather similar in control conditions. Our original cell models allowed us to identify a new unobvious link between proteins in the nucleotide metabolism and other cellular pathways. Further analyses of our models, including additional experiments, could help us to better understand some of the roles played by these enzymes.Octavia CadassouPrescillia ForeyChristelle MachonEdoardo PetrottoKamel ChettabMaria Grazia TozziJérôme GuittonCharles DumontetEmeline Cros-PerrialLars Petter JordheimMDPI AGarticleCD73cN-IImetabolic pathwaystranscriptional regulationBiology (General)QH301-705.5ENCells, Vol 10, Iss 2918, p 2918 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CD73 cN-II metabolic pathways transcriptional regulation Biology (General) QH301-705.5
spellingShingle	CD73 cN-II metabolic pathways transcriptional regulation Biology (General) QH301-705.5 Octavia Cadassou Prescillia Forey Christelle Machon Edoardo Petrotto Kamel Chettab Maria Grazia Tozzi Jérôme Guitton Charles Dumontet Emeline Cros-Perrial Lars Petter Jordheim Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
description	Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases cN-II and CD73, together with RNA sequencing and targeted metabolomics, to decipher new regulatory roles of these proteins. We observed important transcriptional modifications between models as well as upon exposure to adenosine. Metabolite content varied differently between cell models in response to adenosine exposure but was rather similar in control conditions. Our original cell models allowed us to identify a new unobvious link between proteins in the nucleotide metabolism and other cellular pathways. Further analyses of our models, including additional experiments, could help us to better understand some of the roles played by these enzymes.
format	article
author	Octavia Cadassou Prescillia Forey Christelle Machon Edoardo Petrotto Kamel Chettab Maria Grazia Tozzi Jérôme Guitton Charles Dumontet Emeline Cros-Perrial Lars Petter Jordheim
author_facet	Octavia Cadassou Prescillia Forey Christelle Machon Edoardo Petrotto Kamel Chettab Maria Grazia Tozzi Jérôme Guitton Charles Dumontet Emeline Cros-Perrial Lars Petter Jordheim
author_sort	Octavia Cadassou
title	Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_short	Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_full	Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_fullStr	Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_full_unstemmed	Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
title_sort	transcriptional and metabolic investigation in 5′-nucleotidase deficient cancer cell lines
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dc853e9872e7486094c5d65cafe12194
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Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines

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