MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope

Minimal photon fluxes (MINFLUX) has enabled molecule-scale resolution in fluorescence microscopy but this had not been shown in standard, broadly applicable microscopy platforms. Here the authors report a solution to allow normal fluorescence microscopy while also providing 1-3 nm 3D resolution.

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Detalles Bibliográficos
Autores principales: Roman Schmidt, Tobias Weihs, Christian A. Wurm, Isabelle Jansen, Jasmin Rehman, Steffen J. Sahl, Stefan W. Hell
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/dc887fa2587b4746875369d1a3177bc1
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Sumario:Minimal photon fluxes (MINFLUX) has enabled molecule-scale resolution in fluorescence microscopy but this had not been shown in standard, broadly applicable microscopy platforms. Here the authors report a solution to allow normal fluorescence microscopy while also providing 1-3 nm 3D resolution.