PmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume

Abstract The survival in freezing temperature for woody plants is exclusively dependent on the perception of coldness and induction of dormancy. CBF/DREB1 transcriptional factors join cold-response conduits and the DAM genes, especially PmDAM6, are well-known regulators of dormancy. Despite the imme...

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Autores principales:	Kai Zhao, Yuzhen Zhou, Sagheer Ahmad, Xue Yong, Xuehua Xie, Yu Han, Yushu Li, Lidan Sun, Qixiang Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dc8963ceea26428889df1d904cbb5377
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spelling	oai:doaj.org-article:dc8963ceea26428889df1d904cbb53772021-12-02T15:09:00ZPmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume10.1038/s41598-018-22537-w2045-2322https://doaj.org/article/dc8963ceea26428889df1d904cbb53772018-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-22537-whttps://doaj.org/toc/2045-2322Abstract The survival in freezing temperature for woody plants is exclusively dependent on the perception of coldness and induction of dormancy. CBF/DREB1 transcriptional factors join cold-response conduits and the DAM genes, especially PmDAM6, are well-known regulators of dormancy. Despite the immense importance, little is documented on the association between CBF proteins and the complexity of the promoter region in PmDAM6 with the function of bud dormancy in P. mume. Therefore, this study was based on the cloning of PmDAM6 and six PmCBFs to evaluate their integral roles in the process of bud development. The consistency of expressions in either vegetative or reproductive buds provided a negative control from PmCBFs to PmDAM6 during the onset of dormancy. Besides, PmCBF5 could form heteromeric complexes with PmDAM1 and PmDAM6. PmCBF1, PmCBF3, and PmDAM4 recognized the promoter of PmDAM6 by the alternative binding sites. Therefore, the interactions of these genes formulated the base of an obvious model to respond to the coldness and engendered dormancy release. Findings of this study will further help the unveil the genetic control of bud dormancy and its augmentation in P. mume and may offer an explanation for the vernalization.Kai ZhaoYuzhen ZhouSagheer AhmadXue YongXuehua XieYu HanYushu LiLidan SunQixiang ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Zhao Yuzhen Zhou Sagheer Ahmad Xue Yong Xuehua Xie Yu Han Yushu Li Lidan Sun Qixiang Zhang PmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume
description	Abstract The survival in freezing temperature for woody plants is exclusively dependent on the perception of coldness and induction of dormancy. CBF/DREB1 transcriptional factors join cold-response conduits and the DAM genes, especially PmDAM6, are well-known regulators of dormancy. Despite the immense importance, little is documented on the association between CBF proteins and the complexity of the promoter region in PmDAM6 with the function of bud dormancy in P. mume. Therefore, this study was based on the cloning of PmDAM6 and six PmCBFs to evaluate their integral roles in the process of bud development. The consistency of expressions in either vegetative or reproductive buds provided a negative control from PmCBFs to PmDAM6 during the onset of dormancy. Besides, PmCBF5 could form heteromeric complexes with PmDAM1 and PmDAM6. PmCBF1, PmCBF3, and PmDAM4 recognized the promoter of PmDAM6 by the alternative binding sites. Therefore, the interactions of these genes formulated the base of an obvious model to respond to the coldness and engendered dormancy release. Findings of this study will further help the unveil the genetic control of bud dormancy and its augmentation in P. mume and may offer an explanation for the vernalization.
format	article
author	Kai Zhao Yuzhen Zhou Sagheer Ahmad Xue Yong Xuehua Xie Yu Han Yushu Li Lidan Sun Qixiang Zhang
author_facet	Kai Zhao Yuzhen Zhou Sagheer Ahmad Xue Yong Xuehua Xie Yu Han Yushu Li Lidan Sun Qixiang Zhang
author_sort	Kai Zhao
title	PmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume
title_short	PmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume
title_full	PmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume
title_fullStr	PmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume
title_full_unstemmed	PmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume
title_sort	pmcbfs synthetically affect pmdam6 by alternative promoter binding and protein complexes towards the dormancy of bud for prunus mume
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/dc8963ceea26428889df1d904cbb5377
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PmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume

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