Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif
Sitansu Sekhar Nanda,1 Seong Soo A An,1 Dong Kee Yi2,3 1Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, 2Department of Chemistry, 3Department of Environmental Engineering, Myongji University, Yongin, South Korea Abstract: In this study, a new method...
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Dove Medical Press
2015
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oai:doaj.org-article:dca2a75efd184562bb5735d0b85c81832021-12-02T07:45:08ZMeasurement of creatinine in human plasma using a functional porous polymer structure sensing motif1178-2013https://doaj.org/article/dca2a75efd184562bb5735d0b85c81832015-08-01T00:00:00Zhttp://www.dovepress.com/measurement-of-creatinine-in-human-plasma-usingnbspa-functional-porous-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sitansu Sekhar Nanda,1 Seong Soo A An,1 Dong Kee Yi2,3 1Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, 2Department of Chemistry, 3Department of Environmental Engineering, Myongji University, Yongin, South Korea Abstract: In this study, a new method for detecting creatinine was developed. This novel sensor comprised of two ionic liquids, poly-lactic-co-glycolic acid (PLGA) and 1-butyl-3-methylimidazolium (BMIM) chloride, in the presence of 2',7'-dichlorofluorescein diacetate (DCFH-DA). PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure. Creatinine within the FPPS rapidly hydrolyzed and released OH-, which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence. The conversion of DCFH to DCF+ resulted in swelling of FPPS and increased solubility. This DCF+-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood. This novel method could be used in diagnostic applications for monitoring individuals with renal dysfunction. Keywords: PLGA, FPPS, creatinine, blood, diagnosisNanda SSAn SSAYi DKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Special Issue on diverse applications in Nano-Theranostics, Pp 93-99 (2015) |
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DOAJ |
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DOAJ |
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EN |
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Medicine (General) R5-920 |
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Medicine (General) R5-920 Nanda SS An SSA Yi DK Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif |
| description |
Sitansu Sekhar Nanda,1 Seong Soo A An,1 Dong Kee Yi2,3 1Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, 2Department of Chemistry, 3Department of Environmental Engineering, Myongji University, Yongin, South Korea Abstract: In this study, a new method for detecting creatinine was developed. This novel sensor comprised of two ionic liquids, poly-lactic-co-glycolic acid (PLGA) and 1-butyl-3-methylimidazolium (BMIM) chloride, in the presence of 2',7'-dichlorofluorescein diacetate (DCFH-DA). PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure. Creatinine within the FPPS rapidly hydrolyzed and released OH-, which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence. The conversion of DCFH to DCF+ resulted in swelling of FPPS and increased solubility. This DCF+-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood. This novel method could be used in diagnostic applications for monitoring individuals with renal dysfunction. Keywords: PLGA, FPPS, creatinine, blood, diagnosis |
| format |
article |
| author |
Nanda SS An SSA Yi DK |
| author_facet |
Nanda SS An SSA Yi DK |
| author_sort |
Nanda SS |
| title |
Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif |
| title_short |
Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif |
| title_full |
Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif |
| title_fullStr |
Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif |
| title_full_unstemmed |
Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif |
| title_sort |
measurement of creatinine in human plasma using a functional porous polymer structure sensing motif |
| publisher |
Dove Medical Press |
| publishDate |
2015 |
| url |
https://doaj.org/article/dca2a75efd184562bb5735d0b85c8183 |
| work_keys_str_mv |
AT nandass measurementofcreatinineinhumanplasmausingnbspafunctionalporouspolymerstructuresensingmotif AT anssa measurementofcreatinineinhumanplasmausingnbspafunctionalporouspolymerstructuresensingmotif AT yidk measurementofcreatinineinhumanplasmausingnbspafunctionalporouspolymerstructuresensingmotif |
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1718399251078184960 |