Effect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation

Sergio Zaccaria Scalinci1, Lucia Scorolli1, Alessandro Meduri2, Pier Luigi Grenga3, Giulia Corradetti1, Cristian Metrangolo11Low Vision Center &ndash; University of Bologna, Bologna, Italy; 2Department of Surgical Specialities, Ophthalmology Clinic, University of Messina, Messina, Italy; 3De...

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Autores principales:	Sergio Zaccaria Scalinci, Lucia Scorolli, Alessandro Meduri, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/dcab1722070342bb9465197511b13aaa
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spelling	oai:doaj.org-article:dcab1722070342bb9465197511b13aaa2021-12-02T08:59:23ZEffect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation1177-54671177-5483https://doaj.org/article/dcab1722070342bb9465197511b13aaa2011-02-01T00:00:00Zhttp://www.dovepress.com/effect-of-basic-fibroblast-growth-factor-and-cytochrome-c-peroxidase-c-a6367https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Sergio Zaccaria Scalinci1, Lucia Scorolli1, Alessandro Meduri2, Pier Luigi Grenga3, Giulia Corradetti1, Cristian Metrangolo11Low Vision Center &ndash; University of Bologna, Bologna, Italy; 2Department of Surgical Specialities, Ophthalmology Clinic, University of Messina, Messina, Italy; 3Department of Ophthalmology, University of Rome &quot;La Sapienza&quot;, Rome, ItalyPurpose: To evaluate the role of prepared basic fibroblast growth factor (bFGF) and cytochrome c peroxidase (CCP) combination eyedrops in corneal epithelial healing of transgenic mice (B6(A)-Rperd12/J ) after excimer laser photoablation. Materials and methods: In this prospective study, 216 eyes of 108 mice underwent bilateral photorefractive keratectomy. We considered 4 groups: A, B, C, and D. Group A received standard topical postoperative therapy with tobramycin, diclofenac, and dexamethasone eyedrops plus CCP at 3 drops per day for a week or until corneal re-epithelialization was achieved. Group B received standard topical postoperative therapy plus bFGF eyedrops and phosphate-buffered saline (PBS) 3 drops per day for a week or until corneal re-epithelialization was complete. In group C, 1 eye received standard topical postoperative therapy plus CCP eyedrops, bFGF eyedrops, and PBS 3 drops per day for a week or until corneal re-epithelialization was complete. Control eyes (group D) received a standard topical postoperative therapy plus placebo eyedrops. Mice were followed-up for a week from the day after the surgery to evaluate the rate of corneal re-epithelialization.Results: Data were analyzed by ANOVA using the XLSTAT 2010 software. Eyes in group A, B, and C healed completely before the fifth postoperative day, achieving, respectively, a re-epithelialization time of 92 hours &plusmn; 10 SD, 90 hours &plusmn; 12 SD, and 86 hours &plusmn; 12 SD. Group D had a re-epithelialization time of 121 hours &plusmn; 8 SD (P &lt; 0.05). No side effects or toxic effects were documented.Conclusions: Results suggest that re-epithelialization after phototherapeutic keratectomy can benefit from topical therapy with CCP/bFGF combination eyedrops. Further clinical studies are needed to evaluate the long-term effectiveness of these eyedrops to prevent corneal haze.Keywords: cytochrome c peroxidase, bFGF, corneal wound healing, excimer laser photoablation, transgenic mice, refractive surgery Sergio Zaccaria ScalinciLucia ScorolliAlessandro Meduriet alDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2011, Iss default, Pp 215-221 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Sergio Zaccaria Scalinci Lucia Scorolli Alessandro Meduri et al Effect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation
description	Sergio Zaccaria Scalinci1, Lucia Scorolli1, Alessandro Meduri2, Pier Luigi Grenga3, Giulia Corradetti1, Cristian Metrangolo11Low Vision Center &ndash; University of Bologna, Bologna, Italy; 2Department of Surgical Specialities, Ophthalmology Clinic, University of Messina, Messina, Italy; 3Department of Ophthalmology, University of Rome &quot;La Sapienza&quot;, Rome, ItalyPurpose: To evaluate the role of prepared basic fibroblast growth factor (bFGF) and cytochrome c peroxidase (CCP) combination eyedrops in corneal epithelial healing of transgenic mice (B6(A)-Rperd12/J ) after excimer laser photoablation. Materials and methods: In this prospective study, 216 eyes of 108 mice underwent bilateral photorefractive keratectomy. We considered 4 groups: A, B, C, and D. Group A received standard topical postoperative therapy with tobramycin, diclofenac, and dexamethasone eyedrops plus CCP at 3 drops per day for a week or until corneal re-epithelialization was achieved. Group B received standard topical postoperative therapy plus bFGF eyedrops and phosphate-buffered saline (PBS) 3 drops per day for a week or until corneal re-epithelialization was complete. In group C, 1 eye received standard topical postoperative therapy plus CCP eyedrops, bFGF eyedrops, and PBS 3 drops per day for a week or until corneal re-epithelialization was complete. Control eyes (group D) received a standard topical postoperative therapy plus placebo eyedrops. Mice were followed-up for a week from the day after the surgery to evaluate the rate of corneal re-epithelialization.Results: Data were analyzed by ANOVA using the XLSTAT 2010 software. Eyes in group A, B, and C healed completely before the fifth postoperative day, achieving, respectively, a re-epithelialization time of 92 hours &plusmn; 10 SD, 90 hours &plusmn; 12 SD, and 86 hours &plusmn; 12 SD. Group D had a re-epithelialization time of 121 hours &plusmn; 8 SD (P &lt; 0.05). No side effects or toxic effects were documented.Conclusions: Results suggest that re-epithelialization after phototherapeutic keratectomy can benefit from topical therapy with CCP/bFGF combination eyedrops. Further clinical studies are needed to evaluate the long-term effectiveness of these eyedrops to prevent corneal haze.Keywords: cytochrome c peroxidase, bFGF, corneal wound healing, excimer laser photoablation, transgenic mice, refractive surgery
format	article
author	Sergio Zaccaria Scalinci Lucia Scorolli Alessandro Meduri et al
author_facet	Sergio Zaccaria Scalinci Lucia Scorolli Alessandro Meduri et al
author_sort	Sergio Zaccaria Scalinci
title	Effect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation
title_short	Effect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation
title_full	Effect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation
title_fullStr	Effect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation
title_full_unstemmed	Effect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation
title_sort	effect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/dcab1722070342bb9465197511b13aaa
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Effect of basic fibroblast growth factor and cytochrome c peroxidase combination in transgenic mice corneal epithelial healing process after excimer laser photoablation

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