Integrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells

Abstract The identification of cell surface markers specific to pancreatic beta cells is important for both the study of islet biology and for investigating the pathophysiology of diseases in which this cell type is lost or damaged. Following analysis of publicly available RNAseq data, we identified...

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Autores principales:	Jacqueline V. Schiesser, Thomas Loudovaris, Helen E. Thomas, Andrew G. Elefanty, Edouard G. Stanley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dcab1ff3fe334cd4b5b2b617cf023918
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spelling	oai:doaj.org-article:dcab1ff3fe334cd4b5b2b617cf0239182021-12-02T15:51:16ZIntegrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells10.1038/s41598-021-87805-82045-2322https://doaj.org/article/dcab1ff3fe334cd4b5b2b617cf0239182021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87805-8https://doaj.org/toc/2045-2322Abstract The identification of cell surface markers specific to pancreatic beta cells is important for both the study of islet biology and for investigating the pathophysiology of diseases in which this cell type is lost or damaged. Following analysis of publicly available RNAseq data, we identified specific integrin subunits, integrin αv and integrin β5, that were expressed in beta cells. This finding was further elaborated using immunofluorescence analysis of histological sections derived from donor human pancreas. Despite the broad expression of specific integrin subunits, we found that expression of integrin αvβ5 heterodimers was restricted to beta cells and that this complex persisted in islet remnants of some type 1 diabetic individuals from which insulin expression had been lost. This study identifies αvβ5 heterodimers as a novel cell surface marker of human pancreatic beta cells, a finding that will aid in the identification and characterisation of this important cell type.Jacqueline V. SchiesserThomas LoudovarisHelen E. ThomasAndrew G. ElefantyEdouard G. StanleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jacqueline V. Schiesser Thomas Loudovaris Helen E. Thomas Andrew G. Elefanty Edouard G. Stanley Integrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells
description	Abstract The identification of cell surface markers specific to pancreatic beta cells is important for both the study of islet biology and for investigating the pathophysiology of diseases in which this cell type is lost or damaged. Following analysis of publicly available RNAseq data, we identified specific integrin subunits, integrin αv and integrin β5, that were expressed in beta cells. This finding was further elaborated using immunofluorescence analysis of histological sections derived from donor human pancreas. Despite the broad expression of specific integrin subunits, we found that expression of integrin αvβ5 heterodimers was restricted to beta cells and that this complex persisted in islet remnants of some type 1 diabetic individuals from which insulin expression had been lost. This study identifies αvβ5 heterodimers as a novel cell surface marker of human pancreatic beta cells, a finding that will aid in the identification and characterisation of this important cell type.
format	article
author	Jacqueline V. Schiesser Thomas Loudovaris Helen E. Thomas Andrew G. Elefanty Edouard G. Stanley
author_facet	Jacqueline V. Schiesser Thomas Loudovaris Helen E. Thomas Andrew G. Elefanty Edouard G. Stanley
author_sort	Jacqueline V. Schiesser
title	Integrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells
title_short	Integrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells
title_full	Integrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells
title_fullStr	Integrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells
title_full_unstemmed	Integrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells
title_sort	integrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dcab1ff3fe334cd4b5b2b617cf023918
work_keys_str_mv	AT jacquelinevschiesser integrinavb5heterodimerisaspecificmarkerofhumanpancreaticbetacells AT thomasloudovaris integrinavb5heterodimerisaspecificmarkerofhumanpancreaticbetacells AT helenethomas integrinavb5heterodimerisaspecificmarkerofhumanpancreaticbetacells AT andrewgelefanty integrinavb5heterodimerisaspecificmarkerofhumanpancreaticbetacells AT edouardgstanley integrinavb5heterodimerisaspecificmarkerofhumanpancreaticbetacells
_version_	1718385662942511104

Integrin αvβ5 heterodimer is a specific marker of human pancreatic beta cells

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