Circular RNA circPHC3 Promotes Cell Death and Apoptosis in Human BMECs After Oxygen Glucose Deprivation via miR-455-5p/TRAF3 Axis in vitro

Xiaonan Xu, Zimu Wu, Hongyan Qiu, Jun Wu Department of Neurology, Peking University Shenzhen Hospital, Shenzhen 518000, Guangdong Province, People’s Republic of ChinaCorrespondence: Jun Wu Email junwu_2000@126.comObjective: Brain microvascular endothelial cells (BMECs) are involved in brai...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xu X, Wu Z, Qiu H, Wu J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	ischemic stroke circphc3 traf3 mir-455-5p Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/dcabeab54b96417eba9a9a90072e15a4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dcabeab54b96417eba9a9a90072e15a4
record_format	dspace
spelling	oai:doaj.org-article:dcabeab54b96417eba9a9a90072e15a42021-12-02T13:51:18ZCircular RNA circPHC3 Promotes Cell Death and Apoptosis in Human BMECs After Oxygen Glucose Deprivation via miR-455-5p/TRAF3 Axis in vitro1178-2021https://doaj.org/article/dcabeab54b96417eba9a9a90072e15a42021-01-01T00:00:00Zhttps://www.dovepress.com/circular-rna-circphc3-promotes-cell-death-and-apoptosis-in-human-bmecs-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Xiaonan Xu, Zimu Wu, Hongyan Qiu, Jun Wu Department of Neurology, Peking University Shenzhen Hospital, Shenzhen 518000, Guangdong Province, People’s Republic of ChinaCorrespondence: Jun Wu Email junwu_2000@126.comObjective: Brain microvascular endothelial cells (BMECs) are involved in brain vascular dysfunction in ischemic stroke. Abnormal expression of circular RNAs regulate physiological and pathophysiological processes in the central nervous system. The aim of the present study was to investigate profile circRNAs in human BMECs after oxygen glucose deprivation (OGD), which was an in vitro model of ischemic stroke, and find promising biomarkers in ischemic stroke.Methods: RNA sequencing (RNA-seq) technology was conducted to analyze the differential expression of circRNAs between BMECs after OGD and non-OGD treated BMECs. RT-qPCR, cell proliferation, cell apoptosis and dual-luciferase assay, and so on, were used to investigate the functions and molecular mechanisms of hsa_circ_0001360 (named circPHC3 in this study) in ischemic stroke.Results: CircPHC3 was highly expressed in human BMECs after OGD. Knockdown of circPHC3 inhibited cell death and apoptosis in human BMECs treated with OGD. Mechanistically, circPHC3 acted as miR-455-5p sponge to activate TRAF3 to promote cell death and apoptosis in human BMECs after OGD.Conclusion: In short, circPHC3 promotes cell death and apoptosis in ischemic stroke in vitro model, which might be a novel molecular target for acute cerebrovascular protection.Keywords: ischemic stroke, circPHC3, TRAF3, miR-455-5pXu XWu ZQiu HWu JDove Medical Pressarticleischemic strokecircphc3traf3mir-455-5pNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 17, Pp 147-156 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ischemic stroke circphc3 traf3 mir-455-5p Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	ischemic stroke circphc3 traf3 mir-455-5p Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Xu X Wu Z Qiu H Wu J Circular RNA circPHC3 Promotes Cell Death and Apoptosis in Human BMECs After Oxygen Glucose Deprivation via miR-455-5p/TRAF3 Axis in vitro
description	Xiaonan Xu, Zimu Wu, Hongyan Qiu, Jun Wu Department of Neurology, Peking University Shenzhen Hospital, Shenzhen 518000, Guangdong Province, People’s Republic of ChinaCorrespondence: Jun Wu Email junwu_2000@126.comObjective: Brain microvascular endothelial cells (BMECs) are involved in brain vascular dysfunction in ischemic stroke. Abnormal expression of circular RNAs regulate physiological and pathophysiological processes in the central nervous system. The aim of the present study was to investigate profile circRNAs in human BMECs after oxygen glucose deprivation (OGD), which was an in vitro model of ischemic stroke, and find promising biomarkers in ischemic stroke.Methods: RNA sequencing (RNA-seq) technology was conducted to analyze the differential expression of circRNAs between BMECs after OGD and non-OGD treated BMECs. RT-qPCR, cell proliferation, cell apoptosis and dual-luciferase assay, and so on, were used to investigate the functions and molecular mechanisms of hsa_circ_0001360 (named circPHC3 in this study) in ischemic stroke.Results: CircPHC3 was highly expressed in human BMECs after OGD. Knockdown of circPHC3 inhibited cell death and apoptosis in human BMECs treated with OGD. Mechanistically, circPHC3 acted as miR-455-5p sponge to activate TRAF3 to promote cell death and apoptosis in human BMECs after OGD.Conclusion: In short, circPHC3 promotes cell death and apoptosis in ischemic stroke in vitro model, which might be a novel molecular target for acute cerebrovascular protection.Keywords: ischemic stroke, circPHC3, TRAF3, miR-455-5p
format	article
author	Xu X Wu Z Qiu H Wu J
author_facet	Xu X Wu Z Qiu H Wu J
author_sort	Xu X
title	Circular RNA circPHC3 Promotes Cell Death and Apoptosis in Human BMECs After Oxygen Glucose Deprivation via miR-455-5p/TRAF3 Axis in vitro
title_short	Circular RNA circPHC3 Promotes Cell Death and Apoptosis in Human BMECs After Oxygen Glucose Deprivation via miR-455-5p/TRAF3 Axis in vitro
title_full	Circular RNA circPHC3 Promotes Cell Death and Apoptosis in Human BMECs After Oxygen Glucose Deprivation via miR-455-5p/TRAF3 Axis in vitro
title_fullStr	Circular RNA circPHC3 Promotes Cell Death and Apoptosis in Human BMECs After Oxygen Glucose Deprivation via miR-455-5p/TRAF3 Axis in vitro
title_full_unstemmed	Circular RNA circPHC3 Promotes Cell Death and Apoptosis in Human BMECs After Oxygen Glucose Deprivation via miR-455-5p/TRAF3 Axis in vitro
title_sort	circular rna circphc3 promotes cell death and apoptosis in human bmecs after oxygen glucose deprivation via mir-455-5p/traf3 axis in vitro
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/dcabeab54b96417eba9a9a90072e15a4
work_keys_str_mv	AT xux circularrnacircphc3promotescelldeathandapoptosisinhumanbmecsafteroxygenglucosedeprivationviamir4555ptraf3axisinvitro AT wuz circularrnacircphc3promotescelldeathandapoptosisinhumanbmecsafteroxygenglucosedeprivationviamir4555ptraf3axisinvitro AT qiuh circularrnacircphc3promotescelldeathandapoptosisinhumanbmecsafteroxygenglucosedeprivationviamir4555ptraf3axisinvitro AT wuj circularrnacircphc3promotescelldeathandapoptosisinhumanbmecsafteroxygenglucosedeprivationviamir4555ptraf3axisinvitro
_version_	1718392408976130048

Circular RNA circPHC3 Promotes Cell Death and Apoptosis in Human BMECs After Oxygen Glucose Deprivation via miR-455-5p/TRAF3 Axis in vitro

Ejemplares similares