Zones of cellular damage around pulsed-laser wounds.

After a tissue is wounded, cells surrounding the wound adopt distinct wound-healing behaviors to repair the tissue. Considerable effort has been spent on understanding the signaling pathways that regulate immune and tissue-resident cells as they respond to wounds, but these signals must ultimately o...

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Autores principales:	James O'Connor, Fabiha Bushra Akbar, M Shane Hutson, Andrea Page-McCaw
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dcac52a1c43c4a0f94ab788d7d47e1e5
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id	oai:doaj.org-article:dcac52a1c43c4a0f94ab788d7d47e1e5
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spelling	oai:doaj.org-article:dcac52a1c43c4a0f94ab788d7d47e1e52021-12-02T20:07:59ZZones of cellular damage around pulsed-laser wounds.1932-620310.1371/journal.pone.0253032https://doaj.org/article/dcac52a1c43c4a0f94ab788d7d47e1e52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253032https://doaj.org/toc/1932-6203After a tissue is wounded, cells surrounding the wound adopt distinct wound-healing behaviors to repair the tissue. Considerable effort has been spent on understanding the signaling pathways that regulate immune and tissue-resident cells as they respond to wounds, but these signals must ultimately originate from the physical damage inflicted by the wound. Tissue wounds comprise several types of cellular damage, and recent work indicates that different types of cellular damage initiate different types of signaling. Hence to understand wound signaling, it is important to identify and localize the types of wound-induced cellular damage. Laser ablation is widely used by researchers to create reproducible, aseptic wounds in a tissue that can be live-imaged. Because laser wounding involves a combination of photochemical, photothermal and photomechanical mechanisms, each with distinct spatial dependencies, cells around a pulsed-laser wound will experience a gradient of damage. Here we exploit this gradient to create a map of wound-induced cellular damage. Using genetically-encoded fluorescent proteins, we monitor damaged cellular and sub-cellular components of epithelial cells in living Drosophila pupae in the seconds to minutes following wounding. We hypothesized that the regions of damage would be predictably arrayed around wounds of varying sizes, and subsequent analysis found that all damage radii are linearly related over a 3-fold range of wound size. Thus, around laser wounds, the distinct regions of damage can be estimated after measuring any one. This report identifies several different types of cellular damage within a wounded epithelial tissue in a living animal. By quantitatively mapping the size and placement of these different types of damage, we set the foundation for tracing wound-induced signaling back to the damage that initiates it.James O'ConnorFabiha Bushra AkbarM Shane HutsonAndrea Page-McCawPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0253032 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q James O'Connor Fabiha Bushra Akbar M Shane Hutson Andrea Page-McCaw Zones of cellular damage around pulsed-laser wounds.
description	After a tissue is wounded, cells surrounding the wound adopt distinct wound-healing behaviors to repair the tissue. Considerable effort has been spent on understanding the signaling pathways that regulate immune and tissue-resident cells as they respond to wounds, but these signals must ultimately originate from the physical damage inflicted by the wound. Tissue wounds comprise several types of cellular damage, and recent work indicates that different types of cellular damage initiate different types of signaling. Hence to understand wound signaling, it is important to identify and localize the types of wound-induced cellular damage. Laser ablation is widely used by researchers to create reproducible, aseptic wounds in a tissue that can be live-imaged. Because laser wounding involves a combination of photochemical, photothermal and photomechanical mechanisms, each with distinct spatial dependencies, cells around a pulsed-laser wound will experience a gradient of damage. Here we exploit this gradient to create a map of wound-induced cellular damage. Using genetically-encoded fluorescent proteins, we monitor damaged cellular and sub-cellular components of epithelial cells in living Drosophila pupae in the seconds to minutes following wounding. We hypothesized that the regions of damage would be predictably arrayed around wounds of varying sizes, and subsequent analysis found that all damage radii are linearly related over a 3-fold range of wound size. Thus, around laser wounds, the distinct regions of damage can be estimated after measuring any one. This report identifies several different types of cellular damage within a wounded epithelial tissue in a living animal. By quantitatively mapping the size and placement of these different types of damage, we set the foundation for tracing wound-induced signaling back to the damage that initiates it.
format	article
author	James O'Connor Fabiha Bushra Akbar M Shane Hutson Andrea Page-McCaw
author_facet	James O'Connor Fabiha Bushra Akbar M Shane Hutson Andrea Page-McCaw
author_sort	James O'Connor
title	Zones of cellular damage around pulsed-laser wounds.
title_short	Zones of cellular damage around pulsed-laser wounds.
title_full	Zones of cellular damage around pulsed-laser wounds.
title_fullStr	Zones of cellular damage around pulsed-laser wounds.
title_full_unstemmed	Zones of cellular damage around pulsed-laser wounds.
title_sort	zones of cellular damage around pulsed-laser wounds.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/dcac52a1c43c4a0f94ab788d7d47e1e5
work_keys_str_mv	AT jamesoconnor zonesofcellulardamagearoundpulsedlaserwounds AT fabihabushraakbar zonesofcellulardamagearoundpulsedlaserwounds AT mshanehutson zonesofcellulardamagearoundpulsedlaserwounds AT andreapagemccaw zonesofcellulardamagearoundpulsedlaserwounds
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Zones of cellular damage around pulsed-laser wounds.

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