Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly

“Cu-DNAzyme” and “G4-DNAzyme” were used to develop a “turn-off” dual-DNAzyme colorimetric biosensor, which could be used to detect Cu<sup>2+</sup> by employing exonuclease III-mediated cyclical assembly (EMCA). EMCA was based on the cleavage activity of Cu<sup>2+</sup> to tra...

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Autores principales:	Baiqiang Zhai, Kunlun Huang, Hongtao Wang, Dongmin Su, Yuancong Xu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	copper ions dual DNAzyme exonuclease III-mediated cyclical assembly biosensor Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/dcad7dc5a1b74831aacaaf4fe6762833
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spelling	oai:doaj.org-article:dcad7dc5a1b74831aacaaf4fe67628332021-11-25T17:06:14ZHighly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly10.3390/catal111113522073-4344https://doaj.org/article/dcad7dc5a1b74831aacaaf4fe67628332021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4344/11/11/1352https://doaj.org/toc/2073-4344“Cu-DNAzyme” and “G4-DNAzyme” were used to develop a “turn-off” dual-DNAzyme colorimetric biosensor, which could be used to detect Cu<sup>2+</sup> by employing exonuclease III-mediated cyclical assembly (EMCA). EMCA was based on the cleavage activity of Cu<sup>2+</sup> to transfer the linkage sequences of the substrate strand and enzyme strand into the transition sequence. The horseradish peroxidase (HRP)-mimicking activity of the G4-DNAzyme was lost after binding with the complementary transition sequence and was hydrolyzed by <i><b>Exo</b></i> III. These results demonstrate that the proposed colorimetric biosensor was an effective method for ultradetection of trace metals in a high original signal background. Due to the high sensitivity of the biosensor, the limit of detection (LOD) of Cu<sup>2+</sup> is 0.16 nM. This design offers a general purpose platform that could be applied for the detection of any metal ion target through adjustment of metal-dependent DNA-cleaving DNAzymes, which is of great significance for the rapid determination of food safety.Baiqiang ZhaiKunlun HuangHongtao WangDongmin SuYuancong XuMDPI AGarticlecopper ionsdual DNAzymeexonuclease III-mediated cyclical assemblybiosensorChemical technologyTP1-1185ChemistryQD1-999ENCatalysts, Vol 11, Iss 1352, p 1352 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	copper ions dual DNAzyme exonuclease III-mediated cyclical assembly biosensor Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	copper ions dual DNAzyme exonuclease III-mediated cyclical assembly biosensor Chemical technology TP1-1185 Chemistry QD1-999 Baiqiang Zhai Kunlun Huang Hongtao Wang Dongmin Su Yuancong Xu Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly
description	“Cu-DNAzyme” and “G4-DNAzyme” were used to develop a “turn-off” dual-DNAzyme colorimetric biosensor, which could be used to detect Cu<sup>2+</sup> by employing exonuclease III-mediated cyclical assembly (EMCA). EMCA was based on the cleavage activity of Cu<sup>2+</sup> to transfer the linkage sequences of the substrate strand and enzyme strand into the transition sequence. The horseradish peroxidase (HRP)-mimicking activity of the G4-DNAzyme was lost after binding with the complementary transition sequence and was hydrolyzed by <i><b>Exo</b></i> III. These results demonstrate that the proposed colorimetric biosensor was an effective method for ultradetection of trace metals in a high original signal background. Due to the high sensitivity of the biosensor, the limit of detection (LOD) of Cu<sup>2+</sup> is 0.16 nM. This design offers a general purpose platform that could be applied for the detection of any metal ion target through adjustment of metal-dependent DNA-cleaving DNAzymes, which is of great significance for the rapid determination of food safety.
format	article
author	Baiqiang Zhai Kunlun Huang Hongtao Wang Dongmin Su Yuancong Xu
author_facet	Baiqiang Zhai Kunlun Huang Hongtao Wang Dongmin Su Yuancong Xu
author_sort	Baiqiang Zhai
title	Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly
title_short	Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly
title_full	Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly
title_fullStr	Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly
title_full_unstemmed	Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly
title_sort	highly sensitive and selective copper (ii)-catalyzed dual-dnazyme colorimetric biosensor based on exonuclease iii-mediated cyclical assembly
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dcad7dc5a1b74831aacaaf4fe6762833
work_keys_str_mv	AT baiqiangzhai highlysensitiveandselectivecopperiicatalyzeddualdnazymecolorimetricbiosensorbasedonexonucleaseiiimediatedcyclicalassembly AT kunlunhuang highlysensitiveandselectivecopperiicatalyzeddualdnazymecolorimetricbiosensorbasedonexonucleaseiiimediatedcyclicalassembly AT hongtaowang highlysensitiveandselectivecopperiicatalyzeddualdnazymecolorimetricbiosensorbasedonexonucleaseiiimediatedcyclicalassembly AT dongminsu highlysensitiveandselectivecopperiicatalyzeddualdnazymecolorimetricbiosensorbasedonexonucleaseiiimediatedcyclicalassembly AT yuancongxu highlysensitiveandselectivecopperiicatalyzeddualdnazymecolorimetricbiosensorbasedonexonucleaseiiimediatedcyclicalassembly
_version_	1718412745527787520

Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly

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