Cysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients

Solomon Tsegaye,1 Mohammed Mehdi,2 Wajana L Labisso,3 Daniel Seifu Melka2 1Department of Biochemistry, College of Health Sciences, Assela University, Addis Ababa, Ethiopia; 2Department of Biochemistry, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Patholog...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tsegaye S, Mehdi M, Lako WL, Melka DS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	breast cancer cathepsin k cathepsin l mmp-9 mmp-2 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/dcaeae46394c4bee96ca2b6eed61ead3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dcaeae46394c4bee96ca2b6eed61ead3
record_format	dspace
spelling	oai:doaj.org-article:dcaeae46394c4bee96ca2b6eed61ead32021-12-02T14:41:57ZCysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients1179-1314https://doaj.org/article/dcaeae46394c4bee96ca2b6eed61ead32021-05-01T00:00:00Zhttps://www.dovepress.com/cysteine-cathepsins-and-matrix-metalloproteases-among-breast-cancer-pa-peer-reviewed-fulltext-article-BCTThttps://doaj.org/toc/1179-1314Solomon Tsegaye,1 Mohammed Mehdi,2 Wajana L Labisso,3 Daniel Seifu Melka2 1Department of Biochemistry, College of Health Sciences, Assela University, Addis Ababa, Ethiopia; 2Department of Biochemistry, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Pathology, College of Health Sciences, Addis Ababa University, Addis Ababa, EthiopiaCorrespondence: Mohammed MehdiDepartment of Biochemistry, College of Health Sciences, Addis Ababa University, P.o.box: 9086, Addis Ababa, EthiopiaTel +251910066838Email mohammed.mehdi@aau.edu.etBackground: Cellular proteases are thought to increase the likelihood of cancer cell infiltration and metastasis by degrading constituents of the extracellular matrix (ECM). Measuring activities of these proteases may be used as tumor markers for early diagnosis, prognosis, and as a possible target for treatment plan.Objective: The aim of the current study is to evaluate cysteine cathepsins (CTSK and CTSL) and matrix metalloproteases-2 (MMP-2) and 9 (MMP-9) activities in human breast tumor tissue.Methods: A comparative cross-sectional study plan was devised to study the enzymatic activities ofCTSK and CTSL andMMP-2 and MMP-9 via zymographic detection method. Sites of tissue sample collection were St Paul’s Millennium Medical College, Menelik II Hospital and Zewditu Memorial Hospital, Addis Ababa, Ethiopia. A total of 36 breast cancer patients were recruited and tissue samples were collected for the study.Results: Activities of CTSK and CTSL were significantly elevated in cancerous tissue than the adjacent normal non-cancerous breast tissue of the same patients (n = 36, p ≤ 0.05). Also, activities ofMMP-2 and MMP-9 were increased significantly in tumor tissues than normal tissues (n = 36, P ≤ 0.05).Conclusion: It is found that there are different patterns of protease enzymatic activity expression between normal and tumor tissue using zymography. Compared with normal tissue samples, the protease enzymatic activity in cancerous tissue is higher. Thus, tissue proteases can be used in conjunction with histological techniques to identify patients in the same clinical group.Keywords: breast cancer, cathepsin K; CTSK, cathepsin L; CTSL, matrix metalloprotinease-9; MMP-9, matrix metalloprotinease-2; MMP-2Tsegaye SMehdi MLako WLMelka DSDove Medical Pressarticlebreast cancercathepsin kcathepsin lmmp-9mmp-2Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBreast Cancer: Targets and Therapy, Vol Volume 13, Pp 271-283 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer cathepsin k cathepsin l mmp-9 mmp-2 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	breast cancer cathepsin k cathepsin l mmp-9 mmp-2 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Tsegaye S Mehdi M Lako WL Melka DS Cysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients
description	Solomon Tsegaye,1 Mohammed Mehdi,2 Wajana L Labisso,3 Daniel Seifu Melka2 1Department of Biochemistry, College of Health Sciences, Assela University, Addis Ababa, Ethiopia; 2Department of Biochemistry, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Pathology, College of Health Sciences, Addis Ababa University, Addis Ababa, EthiopiaCorrespondence: Mohammed MehdiDepartment of Biochemistry, College of Health Sciences, Addis Ababa University, P.o.box: 9086, Addis Ababa, EthiopiaTel +251910066838Email mohammed.mehdi@aau.edu.etBackground: Cellular proteases are thought to increase the likelihood of cancer cell infiltration and metastasis by degrading constituents of the extracellular matrix (ECM). Measuring activities of these proteases may be used as tumor markers for early diagnosis, prognosis, and as a possible target for treatment plan.Objective: The aim of the current study is to evaluate cysteine cathepsins (CTSK and CTSL) and matrix metalloproteases-2 (MMP-2) and 9 (MMP-9) activities in human breast tumor tissue.Methods: A comparative cross-sectional study plan was devised to study the enzymatic activities ofCTSK and CTSL andMMP-2 and MMP-9 via zymographic detection method. Sites of tissue sample collection were St Paul’s Millennium Medical College, Menelik II Hospital and Zewditu Memorial Hospital, Addis Ababa, Ethiopia. A total of 36 breast cancer patients were recruited and tissue samples were collected for the study.Results: Activities of CTSK and CTSL were significantly elevated in cancerous tissue than the adjacent normal non-cancerous breast tissue of the same patients (n = 36, p ≤ 0.05). Also, activities ofMMP-2 and MMP-9 were increased significantly in tumor tissues than normal tissues (n = 36, P ≤ 0.05).Conclusion: It is found that there are different patterns of protease enzymatic activity expression between normal and tumor tissue using zymography. Compared with normal tissue samples, the protease enzymatic activity in cancerous tissue is higher. Thus, tissue proteases can be used in conjunction with histological techniques to identify patients in the same clinical group.Keywords: breast cancer, cathepsin K; CTSK, cathepsin L; CTSL, matrix metalloprotinease-9; MMP-9, matrix metalloprotinease-2; MMP-2
format	article
author	Tsegaye S Mehdi M Lako WL Melka DS
author_facet	Tsegaye S Mehdi M Lako WL Melka DS
author_sort	Tsegaye S
title	Cysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients
title_short	Cysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients
title_full	Cysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients
title_fullStr	Cysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients
title_full_unstemmed	Cysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients
title_sort	cysteine cathepsins and matrix metalloproteases among breast cancer patients
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/dcaeae46394c4bee96ca2b6eed61ead3
work_keys_str_mv	AT tsegayes cysteinecathepsinsandmatrixmetalloproteasesamongbreastcancerpatients AT mehdim cysteinecathepsinsandmatrixmetalloproteasesamongbreastcancerpatients AT lakowl cysteinecathepsinsandmatrixmetalloproteasesamongbreastcancerpatients AT melkads cysteinecathepsinsandmatrixmetalloproteasesamongbreastcancerpatients
_version_	1718389840798547968

Cysteine Cathepsins and Matrix Metalloproteases Among Breast Cancer Patients

Ejemplares similares