Identification of New Proteins and Potential Mitochondrial F1F0-ATPase Inhibitor Factor 1-Associated Mechanisms in Arabidopsis thaliana Using iTRAQ-Based Quantitative Proteomic Analysis

The mitochondrial synthesis of ATP makes a vital contribution to the growth and development of biological organisms, in which the enzyme mitochondrial F1F0-ATP synthase plays a pivotal role, in that it can either synthesize or hydrolyze cellular ATP. The...

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Autores principales:	Cuiting Chen, Yiqing Meng, Zhongyuan Hu, Jinghua Yang, Mingfang Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	iTRAQ proteomic analysis AtIF1 fertility <i>Arabidopsis thaliana</i> energy Botany QK1-989
Acceso en línea:	https://doaj.org/article/dcb19cf1b95646e3b561e76b6c81b163
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spelling	oai:doaj.org-article:dcb19cf1b95646e3b561e76b6c81b1632021-11-25T18:46:07ZIdentification of New Proteins and Potential Mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase Inhibitor Factor 1-Associated Mechanisms in <i>Arabidopsis thaliana</i> Using iTRAQ-Based Quantitative Proteomic Analysis10.3390/plants101123852223-7747https://doaj.org/article/dcb19cf1b95646e3b561e76b6c81b1632021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2385https://doaj.org/toc/2223-7747The mitochondrial synthesis of ATP makes a vital contribution to the growth and development of biological organisms, in which the enzyme mitochondrial F<sub>1</sub>F<sub>0</sub>-ATP synthase plays a pivotal role, in that it can either synthesize or hydrolyze cellular ATP. The finding of our previous study revealed that mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase inhibitor factor 1 (IF1) in <i>Arabidopsis thaliana</i> has a conserved function as an endogenous inhibitor affecting cellular energy status and plays an important role in plant growth and reproduction, particularly in fertility. In this study, to gain an insight into IF1-related traits, we performed isobaric tags for relative and absolute quantitation labeling analysis. In total, 67 of 4778 identified proteins were identified as differentially expressed proteins (DEPs; 59 up-regulated and 8 down-regulated) between wild-type and <i>if1</i> mutant <i>Arabidopsis thaliana</i> seedlings. Gene ontology enrichment analysis revealed that these DEPs were the most significantly enriched in pathways such as “long-day photoperiodism, flowering,” “positive regulation of protein import into chloroplast stroma,” and “pollen sperm cell differentiation,” which are closely associated with reproductive development. Moreover, Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that photosynthesis was the pathway most significantly enriched with DEPs. Collectively, our results revealed a global shift in protein abundance patterns corresponding to AtIF1 mutation, entailing changes in the abundance of multiple key proteins and metabolic processes, which will provide a valuable proteomic foundation for future studies.Cuiting ChenYiqing MengZhongyuan HuJinghua YangMingfang ZhangMDPI AGarticleiTRAQproteomic analysisAtIF1fertility<i>Arabidopsis thaliana</i>energyBotanyQK1-989ENPlants, Vol 10, Iss 2385, p 2385 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	iTRAQ proteomic analysis AtIF1 fertility <i>Arabidopsis thaliana</i> energy Botany QK1-989
spellingShingle	iTRAQ proteomic analysis AtIF1 fertility <i>Arabidopsis thaliana</i> energy Botany QK1-989 Cuiting Chen Yiqing Meng Zhongyuan Hu Jinghua Yang Mingfang Zhang Identification of New Proteins and Potential Mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase Inhibitor Factor 1-Associated Mechanisms in <i>Arabidopsis thaliana</i> Using iTRAQ-Based Quantitative Proteomic Analysis
description	The mitochondrial synthesis of ATP makes a vital contribution to the growth and development of biological organisms, in which the enzyme mitochondrial F<sub>1</sub>F<sub>0</sub>-ATP synthase plays a pivotal role, in that it can either synthesize or hydrolyze cellular ATP. The finding of our previous study revealed that mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase inhibitor factor 1 (IF1) in <i>Arabidopsis thaliana</i> has a conserved function as an endogenous inhibitor affecting cellular energy status and plays an important role in plant growth and reproduction, particularly in fertility. In this study, to gain an insight into IF1-related traits, we performed isobaric tags for relative and absolute quantitation labeling analysis. In total, 67 of 4778 identified proteins were identified as differentially expressed proteins (DEPs; 59 up-regulated and 8 down-regulated) between wild-type and <i>if1</i> mutant <i>Arabidopsis thaliana</i> seedlings. Gene ontology enrichment analysis revealed that these DEPs were the most significantly enriched in pathways such as “long-day photoperiodism, flowering,” “positive regulation of protein import into chloroplast stroma,” and “pollen sperm cell differentiation,” which are closely associated with reproductive development. Moreover, Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that photosynthesis was the pathway most significantly enriched with DEPs. Collectively, our results revealed a global shift in protein abundance patterns corresponding to AtIF1 mutation, entailing changes in the abundance of multiple key proteins and metabolic processes, which will provide a valuable proteomic foundation for future studies.
format	article
author	Cuiting Chen Yiqing Meng Zhongyuan Hu Jinghua Yang Mingfang Zhang
author_facet	Cuiting Chen Yiqing Meng Zhongyuan Hu Jinghua Yang Mingfang Zhang
author_sort	Cuiting Chen
title	Identification of New Proteins and Potential Mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase Inhibitor Factor 1-Associated Mechanisms in <i>Arabidopsis thaliana</i> Using iTRAQ-Based Quantitative Proteomic Analysis
title_short	Identification of New Proteins and Potential Mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase Inhibitor Factor 1-Associated Mechanisms in <i>Arabidopsis thaliana</i> Using iTRAQ-Based Quantitative Proteomic Analysis
title_full	Identification of New Proteins and Potential Mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase Inhibitor Factor 1-Associated Mechanisms in <i>Arabidopsis thaliana</i> Using iTRAQ-Based Quantitative Proteomic Analysis
title_fullStr	Identification of New Proteins and Potential Mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase Inhibitor Factor 1-Associated Mechanisms in <i>Arabidopsis thaliana</i> Using iTRAQ-Based Quantitative Proteomic Analysis
title_full_unstemmed	Identification of New Proteins and Potential Mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase Inhibitor Factor 1-Associated Mechanisms in <i>Arabidopsis thaliana</i> Using iTRAQ-Based Quantitative Proteomic Analysis
title_sort	identification of new proteins and potential mitochondrial f<sub>1</sub>f<sub>0</sub>-atpase inhibitor factor 1-associated mechanisms in <i>arabidopsis thaliana</i> using itraq-based quantitative proteomic analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dcb19cf1b95646e3b561e76b6c81b163
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Identification of New Proteins and Potential Mitochondrial F<sub>1</sub>F<sub>0</sub>-ATPase Inhibitor Factor 1-Associated Mechanisms in <i>Arabidopsis thaliana</i> Using iTRAQ-Based Quantitative Proteomic Analysis

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