Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor

Abstract The chronic and low-grade inflammation induced by obesity seem to be the “first hit” to retinopathy associated to diabetes type 2. Herein, we hypothesized that omega-3 fatty acids from flaxseed oil enriched diet disrupt the pro-inflammatory status in the retina, protecting against retinopat...

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Autores principales:	Marcella Neves Dátilo, Marcella Ramos Sant’Ana, Guilherme Pedron Formigari, Patrícia Brito Rodrigues, Leandro Pereira de Moura, Adelino Sanchez Ramos da Silva, Eduardo Rochete Ropelle, José Rodrigo Pauli, Dennys Esper Cintra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	G Protein-coupled Receptors (GPR120) GPR120 Receptor Outer Nuclear (ONL) Fatty Acid Arachidonate Inner Nuclear Layer (INL) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dcb7842fcae54358ba71bc1f82fe2528
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spelling	oai:doaj.org-article:dcb7842fcae54358ba71bc1f82fe25282021-12-02T15:07:56ZOmega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor10.1038/s41598-018-32553-52045-2322https://doaj.org/article/dcb7842fcae54358ba71bc1f82fe25282018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32553-5https://doaj.org/toc/2045-2322Abstract The chronic and low-grade inflammation induced by obesity seem to be the “first hit” to retinopathy associated to diabetes type 2. Herein, we hypothesized that omega-3 fatty acids from flaxseed oil enriched diet disrupt the pro-inflammatory status in the retina, protecting against retinopathy development. For eight weeks under a high-fat diet (HF), several physiological parameters were monitored to follow the metabolic homeostasis disruption. After this period, mice were treated with a HF substituted in part of lard by flaxseed oil (FS) for another eight weeks. Food behavior, weight gain, glucose and insulin sensitivity, electroretinography, RT-qPCR and western blots were carried out. The HF was able to induce a pro-inflammatory background in the retina, changing IL1β and TNFα. VEGF, a master piece of retinopathy, had early onset increased also induced by HF. The FS-diet was able to decrease inflammation and retinopathy and improved retinal electro stimuli compared to HF group. GPR120 and GPR40 (G Protein-Coupled Receptors 120 and 40), an omega-3 fatty acid receptors, were detected in the retina for the first time. FS-diet modulated the gene expression and protein content of these receptors. Thus, unsaturated fatty acids protect the retina from diabetes type 2 mice model from disease progression.Marcella Neves DátiloMarcella Ramos Sant’AnaGuilherme Pedron FormigariPatrícia Brito RodriguesLeandro Pereira de MouraAdelino Sanchez Ramos da SilvaEduardo Rochete RopelleJosé Rodrigo PauliDennys Esper CintraNature PortfolioarticleG Protein-coupled Receptors (GPR120)GPR120 ReceptorOuter Nuclear (ONL)Fatty Acid ArachidonateInner Nuclear Layer (INL)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	G Protein-coupled Receptors (GPR120) GPR120 Receptor Outer Nuclear (ONL) Fatty Acid Arachidonate Inner Nuclear Layer (INL) Medicine R Science Q
spellingShingle	G Protein-coupled Receptors (GPR120) GPR120 Receptor Outer Nuclear (ONL) Fatty Acid Arachidonate Inner Nuclear Layer (INL) Medicine R Science Q Marcella Neves Dátilo Marcella Ramos Sant’Ana Guilherme Pedron Formigari Patrícia Brito Rodrigues Leandro Pereira de Moura Adelino Sanchez Ramos da Silva Eduardo Rochete Ropelle José Rodrigo Pauli Dennys Esper Cintra Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor
description	Abstract The chronic and low-grade inflammation induced by obesity seem to be the “first hit” to retinopathy associated to diabetes type 2. Herein, we hypothesized that omega-3 fatty acids from flaxseed oil enriched diet disrupt the pro-inflammatory status in the retina, protecting against retinopathy development. For eight weeks under a high-fat diet (HF), several physiological parameters were monitored to follow the metabolic homeostasis disruption. After this period, mice were treated with a HF substituted in part of lard by flaxseed oil (FS) for another eight weeks. Food behavior, weight gain, glucose and insulin sensitivity, electroretinography, RT-qPCR and western blots were carried out. The HF was able to induce a pro-inflammatory background in the retina, changing IL1β and TNFα. VEGF, a master piece of retinopathy, had early onset increased also induced by HF. The FS-diet was able to decrease inflammation and retinopathy and improved retinal electro stimuli compared to HF group. GPR120 and GPR40 (G Protein-Coupled Receptors 120 and 40), an omega-3 fatty acid receptors, were detected in the retina for the first time. FS-diet modulated the gene expression and protein content of these receptors. Thus, unsaturated fatty acids protect the retina from diabetes type 2 mice model from disease progression.
format	article
author	Marcella Neves Dátilo Marcella Ramos Sant’Ana Guilherme Pedron Formigari Patrícia Brito Rodrigues Leandro Pereira de Moura Adelino Sanchez Ramos da Silva Eduardo Rochete Ropelle José Rodrigo Pauli Dennys Esper Cintra
author_facet	Marcella Neves Dátilo Marcella Ramos Sant’Ana Guilherme Pedron Formigari Patrícia Brito Rodrigues Leandro Pereira de Moura Adelino Sanchez Ramos da Silva Eduardo Rochete Ropelle José Rodrigo Pauli Dennys Esper Cintra
author_sort	Marcella Neves Dátilo
title	Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor
title_short	Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor
title_full	Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor
title_fullStr	Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor
title_full_unstemmed	Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor
title_sort	omega-3 from flaxseed oil protects obese mice against diabetic retinopathy through gpr120 receptor
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/dcb7842fcae54358ba71bc1f82fe2528
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Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor

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