Use of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam

The genetic diversity of foot-and-mouth disease virus (FMDV) poses a challenge to the successful control of the disease, and it is important to identify the emergence of different strains in endemic settings. The objective of this study was to evaluate the sampling of clinically healthy livestock at...

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Autores principales:	Umanga Gunasekara, Miranda R. Bertram, Do H. Dung, Bui H. Hoang, Nguyen T. Phuong, Vo V. Hung, Nguyen V. Long, Phan Q. Minh, Le T. Vu, Pham V. Dong, Andres Perez, Kimberly VanderWaal, Jonathan Arzt
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	genetic diversity phylogenetics subclinical infection molecular epidemiology disease control surveillance Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dcbb4aa49fe445129d25b5be712ccc22
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spelling	oai:doaj.org-article:dcbb4aa49fe445129d25b5be712ccc222021-11-25T19:13:17ZUse of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam10.3390/v131122031999-4915https://doaj.org/article/dcbb4aa49fe445129d25b5be712ccc222021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2203https://doaj.org/toc/1999-4915The genetic diversity of foot-and-mouth disease virus (FMDV) poses a challenge to the successful control of the disease, and it is important to identify the emergence of different strains in endemic settings. The objective of this study was to evaluate the sampling of clinically healthy livestock at slaughterhouses as a strategy for genomic FMDV surveillance. Serum samples (<i>n</i> = 11,875) and oropharyngeal fluid (OPF) samples (<i>n</i> = 5045) were collected from clinically healthy cattle and buffalo on farms in eight provinces in southern and northern Vietnam (2015–2019) to characterize viral diversity. Outbreak sequences were collected between 2009 and 2019. In two slaughterhouses in southern Vietnam, 1200 serum and OPF samples were collected from clinically healthy cattle and buffalo (2017 to 2019) as a pilot study on the use of slaughterhouses as sentinel points in surveillance. FMDV VP1 sequences were analyzed using discriminant principal component analysis and time-scaled phylodynamic trees. Six of seven serotype-O and -A clusters circulating in southern Vietnam between 2017–2019 were detected at least once in slaughterhouses, sometimes pre-dating outbreak sequences associated with the same cluster by 4–6 months. Routine sampling at slaughterhouses may provide a timely and efficient strategy for genomic surveillance to identify circulating and emerging FMDV strains.Umanga GunasekaraMiranda R. BertramDo H. DungBui H. HoangNguyen T. PhuongVo V. HungNguyen V. LongPhan Q. MinhLe T. VuPham V. DongAndres PerezKimberly VanderWaalJonathan ArztMDPI AGarticlegenetic diversityphylogeneticssubclinical infectionmolecular epidemiologydisease controlsurveillanceMicrobiologyQR1-502ENViruses, Vol 13, Iss 2203, p 2203 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic diversity phylogenetics subclinical infection molecular epidemiology disease control surveillance Microbiology QR1-502
spellingShingle	genetic diversity phylogenetics subclinical infection molecular epidemiology disease control surveillance Microbiology QR1-502 Umanga Gunasekara Miranda R. Bertram Do H. Dung Bui H. Hoang Nguyen T. Phuong Vo V. Hung Nguyen V. Long Phan Q. Minh Le T. Vu Pham V. Dong Andres Perez Kimberly VanderWaal Jonathan Arzt Use of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam
description	The genetic diversity of foot-and-mouth disease virus (FMDV) poses a challenge to the successful control of the disease, and it is important to identify the emergence of different strains in endemic settings. The objective of this study was to evaluate the sampling of clinically healthy livestock at slaughterhouses as a strategy for genomic FMDV surveillance. Serum samples (<i>n</i> = 11,875) and oropharyngeal fluid (OPF) samples (<i>n</i> = 5045) were collected from clinically healthy cattle and buffalo on farms in eight provinces in southern and northern Vietnam (2015–2019) to characterize viral diversity. Outbreak sequences were collected between 2009 and 2019. In two slaughterhouses in southern Vietnam, 1200 serum and OPF samples were collected from clinically healthy cattle and buffalo (2017 to 2019) as a pilot study on the use of slaughterhouses as sentinel points in surveillance. FMDV VP1 sequences were analyzed using discriminant principal component analysis and time-scaled phylodynamic trees. Six of seven serotype-O and -A clusters circulating in southern Vietnam between 2017–2019 were detected at least once in slaughterhouses, sometimes pre-dating outbreak sequences associated with the same cluster by 4–6 months. Routine sampling at slaughterhouses may provide a timely and efficient strategy for genomic surveillance to identify circulating and emerging FMDV strains.
format	article
author	Umanga Gunasekara Miranda R. Bertram Do H. Dung Bui H. Hoang Nguyen T. Phuong Vo V. Hung Nguyen V. Long Phan Q. Minh Le T. Vu Pham V. Dong Andres Perez Kimberly VanderWaal Jonathan Arzt
author_facet	Umanga Gunasekara Miranda R. Bertram Do H. Dung Bui H. Hoang Nguyen T. Phuong Vo V. Hung Nguyen V. Long Phan Q. Minh Le T. Vu Pham V. Dong Andres Perez Kimberly VanderWaal Jonathan Arzt
author_sort	Umanga Gunasekara
title	Use of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam
title_short	Use of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam
title_full	Use of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam
title_fullStr	Use of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam
title_full_unstemmed	Use of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam
title_sort	use of slaughterhouses as sentinel points for genomic surveillance of foot-and-mouth disease virus in southern vietnam
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dcbb4aa49fe445129d25b5be712ccc22
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Use of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam

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