Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth

This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures;...

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Autores principales:	El-Dakhly A.T, Youssef M. M., Abeer A.Tammam, Namaa A. Mohamed
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2016
Materias:	vero cell peste des petits ruminants cell growth Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2016.62123 https://doaj.org/article/dcbed62b612043da8d3d7d902a57d2ee
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spelling	oai:doaj.org-article:dcbed62b612043da8d3d7d902a57d2ee2021-12-02T18:08:27ZAssessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growthhttps://dx.doi.org/10.21608/javs.2016.621231687-40722090-3308https://doaj.org/article/dcbed62b612043da8d3d7d902a57d2ee2016-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_62123.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures; the study proved that the optimum time was 24 hours after subculture of VERO cell line using MOI 2: 1. It was also found that the best time of harvstation of virus fluid of PPR was 9th day post inoculation to reach the best titre 6 log10 TCID50 /ml.El-Dakhly A.TYoussef M. M.Abeer A.TammamNamaa A. MohamedEgyptian Society for Animal Managementarticlevero cellpeste des petitsruminantscell growthZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 1, Iss 1, Pp 70-73 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	vero cell peste des petits ruminants cell growth Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	vero cell peste des petits ruminants cell growth Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 El-Dakhly A.T Youssef M. M. Abeer A.Tammam Namaa A. Mohamed Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
description	This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures; the study proved that the optimum time was 24 hours after subculture of VERO cell line using MOI 2: 1. It was also found that the best time of harvstation of virus fluid of PPR was 9th day post inoculation to reach the best titre 6 log10 TCID50 /ml.
format	article
author	El-Dakhly A.T Youssef M. M. Abeer A.Tammam Namaa A. Mohamed
author_facet	El-Dakhly A.T Youssef M. M. Abeer A.Tammam Namaa A. Mohamed
author_sort	El-Dakhly A.T
title	Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
title_short	Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
title_full	Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
title_fullStr	Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
title_full_unstemmed	Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
title_sort	assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
publisher	Egyptian Society for Animal Management
publishDate	2016
url	https://dx.doi.org/10.21608/javs.2016.62123 https://doaj.org/article/dcbed62b612043da8d3d7d902a57d2ee
work_keys_str_mv	AT eldakhlyat assessmentofthepestedespetitsruminantspprattenuatedvirusproducedbyinoculatingverocelllineatdifferentstagesofcellgrowth AT youssefmm assessmentofthepestedespetitsruminantspprattenuatedvirusproducedbyinoculatingverocelllineatdifferentstagesofcellgrowth AT abeeratammam assessmentofthepestedespetitsruminantspprattenuatedvirusproducedbyinoculatingverocelllineatdifferentstagesofcellgrowth AT namaaamohamed assessmentofthepestedespetitsruminantspprattenuatedvirusproducedbyinoculatingverocelllineatdifferentstagesofcellgrowth
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Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth

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