Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill.

Bridelia micrantha is a medicinal plant used traditionally for the treatment of wounds. However, there are no documented scientific reports on the wound healing activities of this plant. This study was conducted to investigate the antimicrobial, antioxidant and wound healing activities of methanol l...

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Autores principales:	Philip Asumang, Yaw Duah Boakye, Theresa Appiah Agana, Jibira Yakubu, Philomena Entsie, William Gariba Akanwariwiak, Francis Adu, Christian Agyare
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Bridelia micrantha Phytochemicals Wounds Antimicrobial Wound healing Antioxidant Science Q
Acceso en línea:	https://doaj.org/article/dcbf278712c44817822fdc3819fd5065
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spelling	oai:doaj.org-article:dcbf278712c44817822fdc3819fd50652021-11-30T04:16:47ZAntimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill.2468-227610.1016/j.sciaf.2021.e00980https://doaj.org/article/dcbf278712c44817822fdc3819fd50652021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2468227621002842https://doaj.org/toc/2468-2276Bridelia micrantha is a medicinal plant used traditionally for the treatment of wounds. However, there are no documented scientific reports on the wound healing activities of this plant. This study was conducted to investigate the antimicrobial, antioxidant and wound healing activities of methanol leaf extract of B. micrantha (BME). The antimicrobial activity was determined using the agar well diffusion and broth dilution methods. Antioxidant activity was performed in vitro using the DPPH scavenging antioxidant activity followed by in vivo wound healing activities using the excision wound model. Preliminary phytochemical screening of BME showed the presence of saponins, tannins, flavonoids, alkaloids, triterpenes, glycosides, steroids and coumarins. BME exhibited dose-dependent antimicrobial activities. The most susceptible organism to BME was N. gonorrhoeae with mean zones of inhibition ranging from 20.50 ± 1.00 to 28.50 ± 1.00 mm. The least susceptible organism was S. typhi with mean zones of inhibition in the range of 15.50 ± 0.00 to 21.50 ± 0.00 mm. The MICs of BME against the test organisms were in the range of 1.25 to 2.5 mg/mL. Sub-inhibitory concentrations of BME potentiated the activities of ciprofloxacin and ketoconazole. BME showed a dose-dependent increase in antioxidant activity with IC50 of 0.2626 µg/mL. The BME formulated creams at concentrations of 0.625, 2.5, and 10% w/w showed a significant (p<0.0001) increase in rate of wound closure compared to the control group. Histological studies revealed marked angiogenesis, collagenation and re-epithelization in BME-treated wound tissues compared to the untreated group. The methanol leaf extract of B. micrantha possess antimicrobial, antioxidant and wound healing activities, thereby proving the folkloric use of the leaves of B. micrantha as a wound-healing agent.Philip AsumangYaw Duah BoakyeTheresa Appiah AganaJibira YakubuPhilomena EntsieWilliam Gariba AkanwariwiakFrancis AduChristian AgyareElsevierarticleBridelia micranthaPhytochemicalsWoundsAntimicrobialWound healingAntioxidantScienceQENScientific African, Vol 14, Iss , Pp e00980- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bridelia micrantha Phytochemicals Wounds Antimicrobial Wound healing Antioxidant Science Q
spellingShingle	Bridelia micrantha Phytochemicals Wounds Antimicrobial Wound healing Antioxidant Science Q Philip Asumang Yaw Duah Boakye Theresa Appiah Agana Jibira Yakubu Philomena Entsie William Gariba Akanwariwiak Francis Adu Christian Agyare Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill.
description	Bridelia micrantha is a medicinal plant used traditionally for the treatment of wounds. However, there are no documented scientific reports on the wound healing activities of this plant. This study was conducted to investigate the antimicrobial, antioxidant and wound healing activities of methanol leaf extract of B. micrantha (BME). The antimicrobial activity was determined using the agar well diffusion and broth dilution methods. Antioxidant activity was performed in vitro using the DPPH scavenging antioxidant activity followed by in vivo wound healing activities using the excision wound model. Preliminary phytochemical screening of BME showed the presence of saponins, tannins, flavonoids, alkaloids, triterpenes, glycosides, steroids and coumarins. BME exhibited dose-dependent antimicrobial activities. The most susceptible organism to BME was N. gonorrhoeae with mean zones of inhibition ranging from 20.50 ± 1.00 to 28.50 ± 1.00 mm. The least susceptible organism was S. typhi with mean zones of inhibition in the range of 15.50 ± 0.00 to 21.50 ± 0.00 mm. The MICs of BME against the test organisms were in the range of 1.25 to 2.5 mg/mL. Sub-inhibitory concentrations of BME potentiated the activities of ciprofloxacin and ketoconazole. BME showed a dose-dependent increase in antioxidant activity with IC50 of 0.2626 µg/mL. The BME formulated creams at concentrations of 0.625, 2.5, and 10% w/w showed a significant (p<0.0001) increase in rate of wound closure compared to the control group. Histological studies revealed marked angiogenesis, collagenation and re-epithelization in BME-treated wound tissues compared to the untreated group. The methanol leaf extract of B. micrantha possess antimicrobial, antioxidant and wound healing activities, thereby proving the folkloric use of the leaves of B. micrantha as a wound-healing agent.
format	article
author	Philip Asumang Yaw Duah Boakye Theresa Appiah Agana Jibira Yakubu Philomena Entsie William Gariba Akanwariwiak Francis Adu Christian Agyare
author_facet	Philip Asumang Yaw Duah Boakye Theresa Appiah Agana Jibira Yakubu Philomena Entsie William Gariba Akanwariwiak Francis Adu Christian Agyare
author_sort	Philip Asumang
title	Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill.
title_short	Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill.
title_full	Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill.
title_fullStr	Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill.
title_full_unstemmed	Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill.
title_sort	antimicrobial, antioxidant and wound healing activities of methanol leaf extract of bridelia micrantha (hochst.) baill.
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/dcbf278712c44817822fdc3819fd5065
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Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill.

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