Two high-rate pentose-phosphate pathways in cancer cells

Abstract The relevant role of pentose phosphate pathway (PPP) in cancer metabolic reprogramming has been usually outlined by studying glucose-6-phosphate dehydrogenase (G6PD). However, recent evidence suggests an unexpected role for a less characterized PPP, triggered by hexose-6-phosphate dehydroge...

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Autores principales:	Vanessa Cossu, Marcella Bonanomi, Matteo Bauckneht, Silvia Ravera, Nicole Righi, Alberto Miceli, Silvia Morbelli, Anna Maria Orengo, Patrizia Piccioli, Silvia Bruno, Daniela Gaglio, Gianmario Sambuceti, Cecilia Marini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dcc05ac72c4a498785c00b45a4a47e1d
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spelling	oai:doaj.org-article:dcc05ac72c4a498785c00b45a4a47e1d2021-12-02T12:40:40ZTwo high-rate pentose-phosphate pathways in cancer cells10.1038/s41598-020-79185-22045-2322https://doaj.org/article/dcc05ac72c4a498785c00b45a4a47e1d2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79185-2https://doaj.org/toc/2045-2322Abstract The relevant role of pentose phosphate pathway (PPP) in cancer metabolic reprogramming has been usually outlined by studying glucose-6-phosphate dehydrogenase (G6PD). However, recent evidence suggests an unexpected role for a less characterized PPP, triggered by hexose-6-phosphate dehydrogenase (H6PD) within the endoplasmic reticulum (ER). Studying H6PD biological role in breast and lung cancer, here we show that gene silencing of this reticular enzyme decreases cell content of PPP intermediates and d-ribose, to a similar extent as G6PD silencing. Decrease in overall NADPH content and increase in cell oxidative status are also comparable. Finally, either gene silencing impairs at a similar degree cell proliferating activity. This unexpected response occurs despite the absence of any cross-interference between the expression of both G6PD and H6PD. Thus, overall cancer PPP reflects the contribution of two different pathways located in the cytosol and ER, respectively. Disregarding the reticular pathway might hamper our comprehension of PPP role in cancer cell biology.Vanessa CossuMarcella BonanomiMatteo BaucknehtSilvia RaveraNicole RighiAlberto MiceliSilvia MorbelliAnna Maria OrengoPatrizia PiccioliSilvia BrunoDaniela GaglioGianmario SambucetiCecilia MariniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vanessa Cossu Marcella Bonanomi Matteo Bauckneht Silvia Ravera Nicole Righi Alberto Miceli Silvia Morbelli Anna Maria Orengo Patrizia Piccioli Silvia Bruno Daniela Gaglio Gianmario Sambuceti Cecilia Marini Two high-rate pentose-phosphate pathways in cancer cells
description	Abstract The relevant role of pentose phosphate pathway (PPP) in cancer metabolic reprogramming has been usually outlined by studying glucose-6-phosphate dehydrogenase (G6PD). However, recent evidence suggests an unexpected role for a less characterized PPP, triggered by hexose-6-phosphate dehydrogenase (H6PD) within the endoplasmic reticulum (ER). Studying H6PD biological role in breast and lung cancer, here we show that gene silencing of this reticular enzyme decreases cell content of PPP intermediates and d-ribose, to a similar extent as G6PD silencing. Decrease in overall NADPH content and increase in cell oxidative status are also comparable. Finally, either gene silencing impairs at a similar degree cell proliferating activity. This unexpected response occurs despite the absence of any cross-interference between the expression of both G6PD and H6PD. Thus, overall cancer PPP reflects the contribution of two different pathways located in the cytosol and ER, respectively. Disregarding the reticular pathway might hamper our comprehension of PPP role in cancer cell biology.
format	article
author	Vanessa Cossu Marcella Bonanomi Matteo Bauckneht Silvia Ravera Nicole Righi Alberto Miceli Silvia Morbelli Anna Maria Orengo Patrizia Piccioli Silvia Bruno Daniela Gaglio Gianmario Sambuceti Cecilia Marini
author_facet	Vanessa Cossu Marcella Bonanomi Matteo Bauckneht Silvia Ravera Nicole Righi Alberto Miceli Silvia Morbelli Anna Maria Orengo Patrizia Piccioli Silvia Bruno Daniela Gaglio Gianmario Sambuceti Cecilia Marini
author_sort	Vanessa Cossu
title	Two high-rate pentose-phosphate pathways in cancer cells
title_short	Two high-rate pentose-phosphate pathways in cancer cells
title_full	Two high-rate pentose-phosphate pathways in cancer cells
title_fullStr	Two high-rate pentose-phosphate pathways in cancer cells
title_full_unstemmed	Two high-rate pentose-phosphate pathways in cancer cells
title_sort	two high-rate pentose-phosphate pathways in cancer cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/dcc05ac72c4a498785c00b45a4a47e1d
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Two high-rate pentose-phosphate pathways in cancer cells

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