The Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays

Cell viability assays require expensive commercial testing kits and sophisticated cell culture techniques. Thus, a laboratory activity for testing the cellular proliferative and cytotoxic effects of certain substances using baker’s yeast (Saccharomyces cerevisiae) in resazurin reduction test (RRT) w...

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Autor principal:	Jayzon G. Bitacura
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dccb9f7aa160460e894154135418f7b1
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spelling	oai:doaj.org-article:dccb9f7aa160460e894154135418f7b12021-11-15T15:04:10ZThe Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays10.1128/jmbe.v19i2.15991935-78851935-7877https://doaj.org/article/dccb9f7aa160460e894154135418f7b12018-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v19i2.1599https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Cell viability assays require expensive commercial testing kits and sophisticated cell culture techniques. Thus, a laboratory activity for testing the cellular proliferative and cytotoxic effects of certain substances using baker’s yeast (Saccharomyces cerevisiae) in resazurin reduction test (RRT) was developed. This activity is based on the fact that a laboratory-prepared resazurin has identical effects to a resazurin-based commercial kits and that S. cerevisiae is an established model system for eukaryotic organisms. Here, a complete procedure based on optimized conditions was described in detail, from preparation of test materials and solutions up to the gathering and analysis of data. This laboratory activity provides a simple and cheap alternative to conventional cell viability assays and is therefore recommended for use in institutions with limited budget for the acquisition of materials for instruction purposes.Jayzon G. BitacuraAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 19, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Jayzon G. Bitacura The Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays
description	Cell viability assays require expensive commercial testing kits and sophisticated cell culture techniques. Thus, a laboratory activity for testing the cellular proliferative and cytotoxic effects of certain substances using baker’s yeast (Saccharomyces cerevisiae) in resazurin reduction test (RRT) was developed. This activity is based on the fact that a laboratory-prepared resazurin has identical effects to a resazurin-based commercial kits and that S. cerevisiae is an established model system for eukaryotic organisms. Here, a complete procedure based on optimized conditions was described in detail, from preparation of test materials and solutions up to the gathering and analysis of data. This laboratory activity provides a simple and cheap alternative to conventional cell viability assays and is therefore recommended for use in institutions with limited budget for the acquisition of materials for instruction purposes.
format	article
author	Jayzon G. Bitacura
author_facet	Jayzon G. Bitacura
author_sort	Jayzon G. Bitacura
title	The Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays
title_short	The Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays
title_full	The Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays
title_fullStr	The Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays
title_full_unstemmed	The Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays
title_sort	use of baker’s yeast in the resazurin reduction test: a simple, low-cost method for determining cell viability in proliferation and cytotoxicity assays
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/dccb9f7aa160460e894154135418f7b1
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The Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays

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