CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
Class 1 CRISPR systems are not as developed for genome editing as Class 2 systems are. Here the authors show that Cas3 can be used to generate functional knockouts and knock-ins, as well as Cas3-mediated exon-skipping in DMD cells.
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Nature Portfolio
2019
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oai:doaj.org-article:dce5c9fe636b4d0abf512a35936827ae2021-12-02T15:36:04ZCRISPR-Cas3 induces broad and unidirectional genome editing in human cells10.1038/s41467-019-13226-x2041-1723https://doaj.org/article/dce5c9fe636b4d0abf512a35936827ae2019-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13226-xhttps://doaj.org/toc/2041-1723Class 1 CRISPR systems are not as developed for genome editing as Class 2 systems are. Here the authors show that Cas3 can be used to generate functional knockouts and knock-ins, as well as Cas3-mediated exon-skipping in DMD cells.Hiroyuki MorisakaKazuto YoshimiYuya OkuzakiPeter GeeYayoi KunihiroEkasit SonphoHuaigeng XuNoriko SasakawaYuki NaitoShinichiro NakadaTakashi YamamotoShigetoshi SanoAkitsu HottaJunji TakedaTomoji MashimoNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Hiroyuki Morisaka Kazuto Yoshimi Yuya Okuzaki Peter Gee Yayoi Kunihiro Ekasit Sonpho Huaigeng Xu Noriko Sasakawa Yuki Naito Shinichiro Nakada Takashi Yamamoto Shigetoshi Sano Akitsu Hotta Junji Takeda Tomoji Mashimo CRISPR-Cas3 induces broad and unidirectional genome editing in human cells |
| description |
Class 1 CRISPR systems are not as developed for genome editing as Class 2 systems are. Here the authors show that Cas3 can be used to generate functional knockouts and knock-ins, as well as Cas3-mediated exon-skipping in DMD cells. |
| format |
article |
| author |
Hiroyuki Morisaka Kazuto Yoshimi Yuya Okuzaki Peter Gee Yayoi Kunihiro Ekasit Sonpho Huaigeng Xu Noriko Sasakawa Yuki Naito Shinichiro Nakada Takashi Yamamoto Shigetoshi Sano Akitsu Hotta Junji Takeda Tomoji Mashimo |
| author_facet |
Hiroyuki Morisaka Kazuto Yoshimi Yuya Okuzaki Peter Gee Yayoi Kunihiro Ekasit Sonpho Huaigeng Xu Noriko Sasakawa Yuki Naito Shinichiro Nakada Takashi Yamamoto Shigetoshi Sano Akitsu Hotta Junji Takeda Tomoji Mashimo |
| author_sort |
Hiroyuki Morisaka |
| title |
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells |
| title_short |
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells |
| title_full |
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells |
| title_fullStr |
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells |
| title_full_unstemmed |
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells |
| title_sort |
crispr-cas3 induces broad and unidirectional genome editing in human cells |
| publisher |
Nature Portfolio |
| publishDate |
2019 |
| url |
https://doaj.org/article/dce5c9fe636b4d0abf512a35936827ae |
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