Bacillus subtilis Attenuates Hepatic and Intestinal Injuries and Modulates Gut Microbiota and Gene Expression Profiles in Mice Infected with Schistosoma japonicum
Parasitic infection can induce pathological injuries and impact the gut microbiota diversity and composition of the host. Bacillus subtilis is a nonpathogenic and noninvasive probiotic bacterium for humans and other animals, playing an important role in improving the host immune system’s ability to...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:dcf107efecd845939ce1f6c5894ff5122021-11-16T05:34:50ZBacillus subtilis Attenuates Hepatic and Intestinal Injuries and Modulates Gut Microbiota and Gene Expression Profiles in Mice Infected with Schistosoma japonicum2296-634X10.3389/fcell.2021.766205https://doaj.org/article/dcf107efecd845939ce1f6c5894ff5122021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.766205/fullhttps://doaj.org/toc/2296-634XParasitic infection can induce pathological injuries and impact the gut microbiota diversity and composition of the host. Bacillus subtilis is a nonpathogenic and noninvasive probiotic bacterium for humans and other animals, playing an important role in improving the host immune system’s ability to respond to intestinal and liver diseases and modulating gut microbiota. However, whether B. subtilis can impact biological functions in Schistosoma japonicum–infected mice is unclear. This study used oral administration (OA) of B. subtilis to treat mice infected with S. japonicum. We evaluated changes in the gut microbiota of infected mice using 16 S rRNA gene sequencing and differentially expressed gene profiles using transcriptome sequencing after OA B. subtilis. We found that OA B. subtilis significantly attenuated hepatic and intestinal pathological injuries in infected mice. The gut microbiota of mice were significantly altered after S. japonicum infection, while OA B. subtilis remodel the diversity and composition of gut microbiomes of infected mice. We found that the S. japonicum–infected mice with OA B. subtilis had an overabundance of the most prevalent bacterial genera, including Bacteroides, Enterococcus, Lactobacillus, Blautia, Lachnoclostridium, Ruminiclostridium, and Enterobacter. Transcriptomic analysis of intestinal tissues revealed that OA B. subtilis shaped the intestinal microenvironment of the host responding to S. japonicum infection. Differentially expressed genes were classified into KEGG pathways between S. japonicum–infected mice and those without included cell adhesion molecules, intestinal immune network for IgA production, hematopoietic cell lineage, Fc epsilon RI signaling pathway, Th1 and Th2 cell differentiation, Th17 cell differentiation, calcium signaling pathway, Fc gamma R-mediated phagocytosis, chemokine signaling pathway, phospholipase D signaling pathway, NF-kappa B signaling pathway, B cell receptor signaling pathway, pancreatic secretion, and phagosome. In conclusion, our findings showed that OA B. subtilis alleviates pathological injuries and regulates gene expression, implying that B. subtilis supplementation may be a potential therapeutic strategy for schistosomiasis. Our study may highlight the value of probiotics as a beneficial supplementary therapy during human schistosomiasis, but further studies are needed.Datao LinDatao LinDatao LinQiuyue SongQiuyue SongQiuyue SongQiuyue SongYishu ZhangYishu ZhangYishu ZhangJiahua LiuJiahua LiuJiahua LiuFang ChenShuling DuShuling DuShuling DuSuoyu XiangSuoyu XiangSuoyu XiangLifu WangLifu WangLifu WangXiaoying WuXiaoying WuXi SunXi SunXi SunFrontiers Media S.A.articleprobioticsSchistosoma japonicumpathological injurygut microbiotatranscriptomicsBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
probiotics Schistosoma japonicum pathological injury gut microbiota transcriptomics Biology (General) QH301-705.5 |
| spellingShingle |
probiotics Schistosoma japonicum pathological injury gut microbiota transcriptomics Biology (General) QH301-705.5 Datao Lin Datao Lin Datao Lin Qiuyue Song Qiuyue Song Qiuyue Song Qiuyue Song Yishu Zhang Yishu Zhang Yishu Zhang Jiahua Liu Jiahua Liu Jiahua Liu Fang Chen Shuling Du Shuling Du Shuling Du Suoyu Xiang Suoyu Xiang Suoyu Xiang Lifu Wang Lifu Wang Lifu Wang Xiaoying Wu Xiaoying Wu Xi Sun Xi Sun Xi Sun Bacillus subtilis Attenuates Hepatic and Intestinal Injuries and Modulates Gut Microbiota and Gene Expression Profiles in Mice Infected with Schistosoma japonicum |
| description |
Parasitic infection can induce pathological injuries and impact the gut microbiota diversity and composition of the host. Bacillus subtilis is a nonpathogenic and noninvasive probiotic bacterium for humans and other animals, playing an important role in improving the host immune system’s ability to respond to intestinal and liver diseases and modulating gut microbiota. However, whether B. subtilis can impact biological functions in Schistosoma japonicum–infected mice is unclear. This study used oral administration (OA) of B. subtilis to treat mice infected with S. japonicum. We evaluated changes in the gut microbiota of infected mice using 16 S rRNA gene sequencing and differentially expressed gene profiles using transcriptome sequencing after OA B. subtilis. We found that OA B. subtilis significantly attenuated hepatic and intestinal pathological injuries in infected mice. The gut microbiota of mice were significantly altered after S. japonicum infection, while OA B. subtilis remodel the diversity and composition of gut microbiomes of infected mice. We found that the S. japonicum–infected mice with OA B. subtilis had an overabundance of the most prevalent bacterial genera, including Bacteroides, Enterococcus, Lactobacillus, Blautia, Lachnoclostridium, Ruminiclostridium, and Enterobacter. Transcriptomic analysis of intestinal tissues revealed that OA B. subtilis shaped the intestinal microenvironment of the host responding to S. japonicum infection. Differentially expressed genes were classified into KEGG pathways between S. japonicum–infected mice and those without included cell adhesion molecules, intestinal immune network for IgA production, hematopoietic cell lineage, Fc epsilon RI signaling pathway, Th1 and Th2 cell differentiation, Th17 cell differentiation, calcium signaling pathway, Fc gamma R-mediated phagocytosis, chemokine signaling pathway, phospholipase D signaling pathway, NF-kappa B signaling pathway, B cell receptor signaling pathway, pancreatic secretion, and phagosome. In conclusion, our findings showed that OA B. subtilis alleviates pathological injuries and regulates gene expression, implying that B. subtilis supplementation may be a potential therapeutic strategy for schistosomiasis. Our study may highlight the value of probiotics as a beneficial supplementary therapy during human schistosomiasis, but further studies are needed. |
| format |
article |
| author |
Datao Lin Datao Lin Datao Lin Qiuyue Song Qiuyue Song Qiuyue Song Qiuyue Song Yishu Zhang Yishu Zhang Yishu Zhang Jiahua Liu Jiahua Liu Jiahua Liu Fang Chen Shuling Du Shuling Du Shuling Du Suoyu Xiang Suoyu Xiang Suoyu Xiang Lifu Wang Lifu Wang Lifu Wang Xiaoying Wu Xiaoying Wu Xi Sun Xi Sun Xi Sun |
| author_facet |
Datao Lin Datao Lin Datao Lin Qiuyue Song Qiuyue Song Qiuyue Song Qiuyue Song Yishu Zhang Yishu Zhang Yishu Zhang Jiahua Liu Jiahua Liu Jiahua Liu Fang Chen Shuling Du Shuling Du Shuling Du Suoyu Xiang Suoyu Xiang Suoyu Xiang Lifu Wang Lifu Wang Lifu Wang Xiaoying Wu Xiaoying Wu Xi Sun Xi Sun Xi Sun |
| author_sort |
Datao Lin |
| title |
Bacillus subtilis Attenuates Hepatic and Intestinal Injuries and Modulates Gut Microbiota and Gene Expression Profiles in Mice Infected with Schistosoma japonicum |
| title_short |
Bacillus subtilis Attenuates Hepatic and Intestinal Injuries and Modulates Gut Microbiota and Gene Expression Profiles in Mice Infected with Schistosoma japonicum |
| title_full |
Bacillus subtilis Attenuates Hepatic and Intestinal Injuries and Modulates Gut Microbiota and Gene Expression Profiles in Mice Infected with Schistosoma japonicum |
| title_fullStr |
Bacillus subtilis Attenuates Hepatic and Intestinal Injuries and Modulates Gut Microbiota and Gene Expression Profiles in Mice Infected with Schistosoma japonicum |
| title_full_unstemmed |
Bacillus subtilis Attenuates Hepatic and Intestinal Injuries and Modulates Gut Microbiota and Gene Expression Profiles in Mice Infected with Schistosoma japonicum |
| title_sort |
bacillus subtilis attenuates hepatic and intestinal injuries and modulates gut microbiota and gene expression profiles in mice infected with schistosoma japonicum |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/dcf107efecd845939ce1f6c5894ff512 |
| work_keys_str_mv |
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