Genomotyping of Coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.

C. burnetii is a Gram-negative intracellular Y-proteobacteria that causes the zoonotic disease Q fever. Q fever can manifest as an acute or chronic illness. Different typing methods have been previously developed to classify C. burnetii isolates to explore its pathogenicity. Here, we report a compre...

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Autores principales:	Quentin Leroy, Fabrice Armougom, Pascal Barbry, Didier Raoult
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dcf65b5501c8468c85f3c0f0b090d64b
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spelling	oai:doaj.org-article:dcf65b5501c8468c85f3c0f0b090d64b2021-11-18T07:35:49ZGenomotyping of Coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.1932-620310.1371/journal.pone.0025781https://doaj.org/article/dcf65b5501c8468c85f3c0f0b090d64b2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22046248/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203C. burnetii is a Gram-negative intracellular Y-proteobacteria that causes the zoonotic disease Q fever. Q fever can manifest as an acute or chronic illness. Different typing methods have been previously developed to classify C. burnetii isolates to explore its pathogenicity. Here, we report a comprehensive genomotyping method based on the presence or absence of genes using microarrays. The genomotyping method was then tested in 52 isolates obtained from different geographic areas, different hosts and patients with different clinical manifestations. The analysis revealed the presence of 10 genomotypes organized into 3 groups, with a topology congruent with that obtained through multi-spacer typing. We also found that only 4 genomotypes were specifically associated with acute Q fever, whereas all of the genomotypes could be associated to chronic human infection. Serendipitously, the genomotyping results revealed that all hard tick isolates, including the Nine Mile strain, belong to the same genomotype.Quentin LeroyFabrice ArmougomPascal BarbryDidier RaoultPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e25781 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Quentin Leroy Fabrice Armougom Pascal Barbry Didier Raoult Genomotyping of Coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.
description	C. burnetii is a Gram-negative intracellular Y-proteobacteria that causes the zoonotic disease Q fever. Q fever can manifest as an acute or chronic illness. Different typing methods have been previously developed to classify C. burnetii isolates to explore its pathogenicity. Here, we report a comprehensive genomotyping method based on the presence or absence of genes using microarrays. The genomotyping method was then tested in 52 isolates obtained from different geographic areas, different hosts and patients with different clinical manifestations. The analysis revealed the presence of 10 genomotypes organized into 3 groups, with a topology congruent with that obtained through multi-spacer typing. We also found that only 4 genomotypes were specifically associated with acute Q fever, whereas all of the genomotypes could be associated to chronic human infection. Serendipitously, the genomotyping results revealed that all hard tick isolates, including the Nine Mile strain, belong to the same genomotype.
format	article
author	Quentin Leroy Fabrice Armougom Pascal Barbry Didier Raoult
author_facet	Quentin Leroy Fabrice Armougom Pascal Barbry Didier Raoult
author_sort	Quentin Leroy
title	Genomotyping of Coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.
title_short	Genomotyping of Coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.
title_full	Genomotyping of Coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.
title_fullStr	Genomotyping of Coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.
title_full_unstemmed	Genomotyping of Coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.
title_sort	genomotyping of coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/dcf65b5501c8468c85f3c0f0b090d64b
work_keys_str_mv	AT quentinleroy genomotypingofcoxiellaburnetiiusingmicroarraysrevealsaconservedgenomotypeforhardtickisolates AT fabricearmougom genomotypingofcoxiellaburnetiiusingmicroarraysrevealsaconservedgenomotypeforhardtickisolates AT pascalbarbry genomotypingofcoxiellaburnetiiusingmicroarraysrevealsaconservedgenomotypeforhardtickisolates AT didierraoult genomotypingofcoxiellaburnetiiusingmicroarraysrevealsaconservedgenomotypeforhardtickisolates
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Genomotyping of Coxiella burnetii using microarrays reveals a conserved genomotype for hard tick isolates.

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