KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity

The potential of KDP, a lactic acid bacterial strain of <i>Lactobacillus sakei</i>, to enhance the production of mucosal specific immunoglobulin A (IgA) in mice and thereby enhance gut mucosal immunity was examined. KDP is composed of dead cells isolated from the Korean traditional food...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mamdooh Ghoneum, Shaymaa Abdulmalek
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	IgA secretory KDP Peyer’s patches OVA albumin antioxidant Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/dcf7c63719004d02a51fca946d220359
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dcf7c63719004d02a51fca946d220359
record_format	dspace
spelling	oai:doaj.org-article:dcf7c63719004d02a51fca946d2203592021-11-25T18:35:26ZKDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity10.3390/nu131139362072-6643https://doaj.org/article/dcf7c63719004d02a51fca946d2203592021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3936https://doaj.org/toc/2072-6643The potential of KDP, a lactic acid bacterial strain of <i>Lactobacillus sakei</i>, to enhance the production of mucosal specific immunoglobulin A (IgA) in mice and thereby enhance gut mucosal immunity was examined. KDP is composed of dead cells isolated from the Korean traditional food kimchi. Female BALB/c mice orally received 0.25 mg KDP once daily for 5 weeks and were co-administrated ovalbumin (OVA) for negative control and cholera toxin for positive control. Mice administered KDP exhibited increased secretory IgA (sIgA) contents in the small intestine, Peyer’s patches, serum, colon, and lungs as examined by ELISA. KDP also significantly increased the gene expression of <i>Bcl-6</i>, <i>IL-10</i>, <i>IL-12p40</i>, <i>IL-21</i>, and <i>STAT4</i>. In addition, KDP acted as a potent antioxidant, as indicated by its significant inhibitory effects in the range of 16.5–59.4% for DPPH, nitric oxide, maximum total antioxidant capacity, and maximum reducing power. Finally, KDP exhibited potent antimicrobial activity as evidenced by a significant decrease in the growth of 7 samples of gram-negative and gram-positive bacteria and <i>Candida albicans</i>. KDP’s adjuvant effect is shown to be comparable to that of cholera toxin. We conclude that KDP can significantly enhance the intestine’s secretory immunity to OVA, as well as act as a potent antioxidant and antimicrobial agent. These results suggest that orally administered KDP should be studied in clinical trials for antigen-specific IgA production.Mamdooh GhoneumShaymaa AbdulmalekMDPI AGarticleIgAsecretoryKDPPeyer’s patchesOVA albuminantioxidantNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3936, p 3936 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	IgA secretory KDP Peyer’s patches OVA albumin antioxidant Nutrition. Foods and food supply TX341-641
spellingShingle	IgA secretory KDP Peyer’s patches OVA albumin antioxidant Nutrition. Foods and food supply TX341-641 Mamdooh Ghoneum Shaymaa Abdulmalek KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity
description	The potential of KDP, a lactic acid bacterial strain of <i>Lactobacillus sakei</i>, to enhance the production of mucosal specific immunoglobulin A (IgA) in mice and thereby enhance gut mucosal immunity was examined. KDP is composed of dead cells isolated from the Korean traditional food kimchi. Female BALB/c mice orally received 0.25 mg KDP once daily for 5 weeks and were co-administrated ovalbumin (OVA) for negative control and cholera toxin for positive control. Mice administered KDP exhibited increased secretory IgA (sIgA) contents in the small intestine, Peyer’s patches, serum, colon, and lungs as examined by ELISA. KDP also significantly increased the gene expression of <i>Bcl-6</i>, <i>IL-10</i>, <i>IL-12p40</i>, <i>IL-21</i>, and <i>STAT4</i>. In addition, KDP acted as a potent antioxidant, as indicated by its significant inhibitory effects in the range of 16.5–59.4% for DPPH, nitric oxide, maximum total antioxidant capacity, and maximum reducing power. Finally, KDP exhibited potent antimicrobial activity as evidenced by a significant decrease in the growth of 7 samples of gram-negative and gram-positive bacteria and <i>Candida albicans</i>. KDP’s adjuvant effect is shown to be comparable to that of cholera toxin. We conclude that KDP can significantly enhance the intestine’s secretory immunity to OVA, as well as act as a potent antioxidant and antimicrobial agent. These results suggest that orally administered KDP should be studied in clinical trials for antigen-specific IgA production.
format	article
author	Mamdooh Ghoneum Shaymaa Abdulmalek
author_facet	Mamdooh Ghoneum Shaymaa Abdulmalek
author_sort	Mamdooh Ghoneum
title	KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity
title_short	KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity
title_full	KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity
title_fullStr	KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity
title_full_unstemmed	KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity
title_sort	kdp, a lactobacilli product from kimchi, enhances mucosal immunity by increasing secretory iga in mice and exhibits antimicrobial activity
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dcf7c63719004d02a51fca946d220359
work_keys_str_mv	AT mamdoohghoneum kdpalactobacilliproductfromkimchienhancesmucosalimmunitybyincreasingsecretoryigainmiceandexhibitsantimicrobialactivity AT shaymaaabdulmalek kdpalactobacilliproductfromkimchienhancesmucosalimmunitybyincreasingsecretoryigainmiceandexhibitsantimicrobialactivity
_version_	1718410983301447680

KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity

Ejemplares similares